Abstract
Oleanolic acid is known as which anti-cancer, anti-sinhaeng angiogenic, anti-inflammatory, antioxidant and anti-wrinkle effects. We focused on the antioxidant activity of oleanolic acid was separated from the natural plant and It was confirmed that the whitening effect. In this study, oleanolic acid was stabilized by polyglyceryl surfactant which from natural origin with only a simple stirring operation, and compared with lecithin liposome that was manufactured with high cost facility. The transdermal transition rate of 0.4% oleanolic acid polyglyceryl nanoemulsion was 95%, and it was simillar with lecithin liposome of 92%. 65% of 3hr transdermal transition rate of polyglyceryl nanoemulsion indicate charistiristcs of quick release, compared with 45% of lecithin liposome's 3hr transdermal transition rate. In the in-vivo clinical trial test, polyglyceryl nanoemulsion of 0.4% oleanolic acid was higher 25% in 2nd week, 58% in 4th and 8th weeks than non-added oleanolic acid emulsion.
올레아놀릭산은 항암, 신행혈관 생성방지, 항염증, 항산화 및 주름 개선효과가 알려져 있다. 본 연구자들은 천연에서 분리한 올레아놀릭산의 항산화효과에 주목하여 연구하였고 미백효과가 있음을 확인하였다. 본 연구에서는 천연유래의 폴리글리세릴계 계면활성제를 사용하여 간단한 교반만으로 올레아놀릭산을 안정화하였고 고가의 장비인 마이크로풀루다이저를이용하여 제조한 레시틴리포좀과 경피흡수투과율을 비교하였다. 0.4% 올레아놀릭산을 안정화한 폴리글리세릴 나노에멀젼의 12시간 후 경피흡수투과율은 95%였다. 레시틴 리포좀은 92%로 유사하였으나 폴리글리세릴 나노에멀젼은 3시간 경피 흡수투과율이 65%로 레시틴리포좀의 45%에 비해 속방성의 특징을 보였다. 인체대상 임상시험결과 올레아놀릭산을 무배합한 대조군에 비해 올레아놀릭산을 배합한 폴리글리세릴 나노에멀젼은 MEXAMETER의한 멜라닌 색소감소효과가 2주차 25%, 4주차 58%, 8주차 58%이상 높았다.