Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Expression of the mexA Gene Requires the DNA Helicase RecG in Pseudomonas aeruginosa PAO1

  • Heo, Aram (Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University) ;
  • Park, Woojun (Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University)
  • Received : 2014.10.06
  • Accepted : 2014.11.10
  • Published : 2015.04.28
Download PDF
⟨ Previous Next ⟩

Abstract

This study provides evidence that RecG regulates the expression of the OxyR-independent gene mexA in Pseudomonas aeruginosa PAO1. A reduction in mexA expression was observed in the absence of RecG, but not OxyR, by northern blot and quantitative real-time PCR analyses. The canonical palindromic RecG binding sequence was present upstream of the mexA promoter, and bound purified RecG and single strand-binding protein. These data reveal a novel mechanism of OxyR-independent gene transcription by RecG.

Keywords

References

  1. Buss JA, Kimura Y, Bianco PR. 2008. RecG interacts directly with SSB: implications for stalled replication fork regression. Nucleic Acids Res. 36: 7029-7042. https://doi.org/10.1093/nar/gkn795
  2. Buyck JM, Guénard S, Plésiat P, Tulkens PM, Van Bambeke F. 2012. Role of MexAB-OprM in intrinsic resistance of Pseudomonas aeruginosa to temocillin and impact on the susceptibility of strains isolated from patients suffering from cystic fibrosis. J. Antimicrob. Chemother. 67: 771-775. https://doi.org/10.1093/jac/dkr543
  3. Cañas C, Suzuki Y, Marchisone C, Carrasco B, Freire-Benéitez V, Takeyasu K, et al. 2014. Interaction of branch migration translocases with the Holliday junction-resolving enzyme and their implications in Holliday junction resolution. J. Biol. Chem. 289: 17634-17646. https://doi.org/10.1074/jbc.M114.552794
  4. Daigle DM, Cao L, Fraud S, Wilke MS, Pacey A, Klinoski R, et al. 2007. Protein modulator of multidrug efflux gene expression in Pseudomonas aeruginosa. J. Bacteriol. 189: 5441-5451. https://doi.org/10.1128/JB.00543-07
  5. Evans K, Adewoye L, Poole K. 2001. MexR repressor of the mexAB-oprM multidrug efflux operon of Pseudomonas aeruginosa: identification of MexR binding sites in the mexA-mexR intergenic region. J. Bacteriol. 183: 807-812. https://doi.org/10.1128/JB.183.3.807-812.2001
  6. Li XZ, Nikaido H, Poole K. 1995. Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa. Antimicrob. Agents. Chemother. 39: 1948-1953. https://doi.org/10.1128/AAC.39.9.1948
  7. Lloyd RG, Buckman C. 1991. Genetic analysis of the recG locus of Escherichia coli K-12 and of its role in recombination and DNA repair. J. Bacteriol. 173: 1004-1011. https://doi.org/10.1128/jb.173.3.1004-1011.1991
  8. Maseda H, Yoneyama H, Nakae T. 2000. Assignment of the substrate-selective subunits of the MexEF-OprN multidrug efflux pump of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 44: 658-664. https://doi.org/10.1128/AAC.44.3.658-664.2000
  9. McGlynn P, Mahdi AA, Lloyd RG. 2000. Characterisation of the catalytically active form of RecG helicase. Nucleic Acids Res. 28: 2324-2332. https://doi.org/10.1093/nar/28.12.2324
  10. Morita Y, Cao L, Gould G, Avison MB, Poole K. 2006. nalD encodes a second repressor of the mexAB-oprM multidrug efflux operon of Pseudomonas aeruginosa. J. Bacteriol. 188: 8649-8654. https://doi.org/10.1128/JB.01342-06
  11. Ochsner UA, Vasil ML, Alsabbagh E, Parvatiyar K, Hassett DJ. 2000. Role of the Pseudomonas aeruginosa oxyR-recG operon in oxidative stress defense and DNA repair: OxyR-dependent regulation of katB-ankB, ahpB, and ahpC-ahpF. J. Bacteriol. 182: 4533-4544. https://doi.org/10.1128/JB.182.16.4533-4544.2000
  12. Ochsner UA, Hassett DJ, Vasil ML. 2001. Genetic and physiological characterization of ohr, encoding a protein involved in organic hydroperoxide resistance in Pseudomonas aeruginosa. J. Bacteriol. 183: 773-778. https://doi.org/10.1128/JB.183.2.773-778.2001
  13. Poole K, Srikumar R. 2001. Multidrug efflux in Pseudomonas aeruginosa: components, mechanisms and clinical significance. Curr. Top. Med. Chem. 1: 59-71. https://doi.org/10.2174/1568026013395605
  14. Sánchez P, Rojo F, Martínez JL. 2002. Transcriptional regulation of mexR, the repressor of Pseudomonas aeruginosa mexAB-oprM multidrug efflux pump. FEMS Microbiol. Lett. 207: 63-68. https://doi.org/10.1111/j.1574-6968.2002.tb11029.x
  15. Sharples GJ, Ingleston SM, Lloyd RG. 1999. Holliday junction processing in bacteria: insights from the evolutionary conservation of RuvABC, RecG, and RusA. J. Bacteriol. 181: 5543-5550.
  16. Yeom J, Lee Y, Park W. 2012. ATP-dependent RecG helicase is required for the transcriptional regulator OxyR function in Pseudomonas species. J. Biol. Chem. 287: 24492-24504. https://doi.org/10.1074/jbc.M112.356964