• Title/Summary/Keyword: Pseudomonas aerruginosa

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Expression of the mexA Gene Requires the DNA Helicase RecG in Pseudomonas aeruginosa PAO1

  • Heo, Aram;Park, Woojun
    • Journal of Microbiology and Biotechnology
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    • v.25 no.4
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    • pp.492-495
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    • 2015
  • This study provides evidence that RecG regulates the expression of the OxyR-independent gene mexA in Pseudomonas aeruginosa PAO1. A reduction in mexA expression was observed in the absence of RecG, but not OxyR, by northern blot and quantitative real-time PCR analyses. The canonical palindromic RecG binding sequence was present upstream of the mexA promoter, and bound purified RecG and single strand-binding protein. These data reveal a novel mechanism of OxyR-independent gene transcription by RecG.