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Early Growth Response-1 Plays a Non-redundant Role in the Differentiation of B Cells into Plasma Cells

  • Oh, Yeon-Kyung (Laboratory of Autoimmunology, Department of Anatomy and Cell Biology, College of Medicine, Hanyang University) ;
  • Jang, Eunkyeong (Laboratory of Autoimmunology, Department of Anatomy and Cell Biology, College of Medicine, Hanyang University) ;
  • Paik, Doo-Jin (Laboratory of Autoimmunology, Department of Anatomy and Cell Biology, College of Medicine, Hanyang University) ;
  • Youn, Jeehee (Laboratory of Autoimmunology, Department of Anatomy and Cell Biology, College of Medicine, Hanyang University)
  • Received : 2015.04.03
  • Accepted : 2015.06.04
  • Published : 2015.06.30
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Abstract

Early growth response (Egr)-1 is a $Cys_2-His_2-type$ zincfinger transcription factor. It has been shown to induce survival and proliferation of immature and mature B cells, respectively, but its role in the differentiation of B cells into plasma cells remains unclear. To examine the effects of Egr-1 deficiency on the activation of B cells, naive B cells from $Egr1^{-/-}$mice and their wild-type (WT) littermates were activated to proliferate and differentiate, and then assayed by FACS. Proportions of cells undergoing proliferation and apoptosis did not differ between $Egr1^{-/-}$ and WT mice. However, $Egr1^{-/-}$ B cells gave rise to fewer plasma cells than WT B cells. Consistently, $Egr1^{-/-}$ mice produced significantly lower titer of antigen-specific IgG than their WT littermates upon immunization. Our results demonstrate that Egr-1 participates in the differentiation program of B cells into plasma cells, while it is dispensable for the proliferation and survival of mature B cells.

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References

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