• Journal of Life Science
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• v.17 no.1 s.81
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• pp.110-115
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• 2007

It has been previously described that transcription factor early growth response gene product 1 (EGR-1) functions as a tumor suppressor gene. This study was conducted to demonstrate that EGR-1 induction by phytochemical apigenin and its derivative isovitexin can mediate the growth suppression of the intestinal epithelial tumor cells. Apigenin and isovitexin induced EGR-1 gene expression both in the dose and time-dependent manners. Moreover the induction was relatively late around 9-12 hr after treatment of HCT-116 cells, while several anti-inflammatory agent such as NSAIDS and catechins elicit the ECR-1 gene expression at much earlier time about 1-3 hr after treatment. In terms of signal transduction, ERK1/2 was critical for apigenin-induced EGR-1 gene expression and its promoter activation. When EGR-1 gene expression was blocked with EGR-1 small interference RNA, the cytotoxicity of apigenin in the human epithelial cells was attenuated, suggesting the involvement of EGR-1 in the anti-tumoric activity of apigenin. To link the EGR-1 induction to EGR-1-regulated gene products in colon cancer, NSAID-Activated Gene 1 (NAG-1) was demonstrated to be elevated by apigenin and isovitexin at 24-48 hr after treatment. Taken together, apigenin-activated ERK1/2 mediated EGR-1 gene induction, which was associated with suppression of the cellular viability by apigenin compound.

• Journal of Life Science
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• v.31 no.4
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• pp.425-429
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• 2021

We have investigated the expression of early growth response protein 1 (EGR1) in INS-1 pancreatic β-cells treated with Allomyrina dichotoma hemolymph. The Korean rhinoceros beetle, A. dichotoma (Coleoptera: Scarabaeidae), is important in the insect industry for medical applications. We have already established a method for purification of A. dichotoma hemolymph that can be used in many experiments. EGR1 is reported as a multifunctional transcription factor that is implicated in virus infections. EGR1 has therefore been revealed as a major mediator and regulator in the physiological and pathological conditions of several cell and tissue types. New findings in this study are that A. dichotoma hemolymph, which promotes a dose- and time-dependent upregulation of EGR1 gene expression, shows an enhancement of this gene expression when combined with hypothermia or endoplasmic reticulum (ER) stress. These results suggest that A. dichotoma hemolymph may provide clues to EGR1-associated disease therapies involving gene regulation of EGR1.

• Development and Reproduction
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• v.18 no.4
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• pp.233-240
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• 2014

The early growth response protein 1 (Egr-1) is a widely reported zinc finger protein and a well known transcription factor encoded by the Egr-1 gene, which plays key roles in many aspects of vertebrate embryogenesis and in adult vertebrates. The Egr-1 expression is important in the formation of the gill vascular system in flounders, which develops during the post-hatching phase and is essential for survival during the juvenile period. However, the complete details of Egr-1 expression during embryo development in olive flounder are not available. We assessed the expression patterns of Egr-1 during the early development of olive flounders by using reverse transcription polymerase chain reaction (RT-PCR) analysis. Microscopic observations showed that gill filament formation corresponded with the Egr-1 expression. Thus, we showed that Egr-1 plays a vital role in angiogenesis in the gill filaments during embryogenesis. Further, Egr-1 expression was found to be strong at 5 days after hatching (DAH), in the development of the gill vascular system, and this strong expression level was maintained throughout all the development stages. Our findings have important implications with respect to the biological role of Egr-1 and evolution of the first respiratory blood vessels in the gills of olive flounder. Further studies are required to elucidate the Egr-1-mediated stress response and to decipher the functional role of Egr-1 in developmental stages.

• BMB Reports
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• v.54 no.10
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• pp.497-504
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• 2021

EGR1 (early growth response 1) is dysregulated in many cancers and exhibits both tumor suppressor and promoter activities, making it an appealing target for cancer therapy. Here, we used a systematic multi-omics analysis to review the expression of EGR1 and its role in regulating clinical outcomes in breast cancer (BC). EGR1 expression, its promoter methylation, and protein expression pattern were assessed using various publicly available tools. COSMIC-based somatic mutations and cBioPortal-based copy number alterations were analyzed, and the prognostic roles of EGR1 in BC were determined using Prognoscan and Kaplan-Meier Plotter. We also used bc-GenEx-Miner to investigate the EGR1 co-expression profile. EGR1 was more often downregulated in BC tissues than in normal breast tissue, and its knockdown was positively correlated with poor survival. Low EGR1 expression levels were also associated with increased risk of ER+, PR+, and HER2- BCs. High positive correlations were observed among EGR1, DUSP1, FOS, FOSB, CYR61, and JUN mRNA expression in BC tissue. This systematic review suggested that EGR1 expression may serve as a prognostic marker for BC patients and that clinicopathological parameters influence its prognostic utility. In addition to EGR1, DUSP1, FOS, FOSB, CYR61, and JUN can jointly be considered prognostic indicators for BC.

• Transactions of the Korean Society of Automotive Engineers
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• v.10 no.1
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• pp.24-31
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• 2002

An experimental study was conducted to investigate the effects of EGR (Exhaust Gas Recirculation) variables on performance and emission characteristics in a 2-liter 4-cylinder spark-ignition LPG fuelled engine. The effects of EGR on the reduction of thermal loading at exhaust manifold were also investigated because the reduced gas temperature is desirable for the reliability of an engine in light of both thermal efficiency and material issue of exhaust manifold. The steady-state tests show that the brake thermal efficiency increased and the brake specific fuel consumption decreased with the increase of EGR rate in hot EGR and with the decrease of EGR temperature in case of cooled EGR, while the stable combustion was maintained. The increase of EGR rate or the decrease of EGR temperature results in the reduction of NOx emission even in the increase of HC emission. Furthermore, decreasing EGR temperature by $180^{\circ}C$ enabled the reduction of exhaust gas temperature by $15^{\circ}C$ in cooled EGR test at 1600rpm/370kPa BMEP operation, and consequently the reduction of thermal load at exhaust. The optimization strategy of EGR application is to be discussed by the investigation on the effect of geometrical characteristics of EGR-supplying pipe line.

• Journal of Advanced Marine Engineering and Technology
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• v.35 no.4
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• pp.379-387
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• 2011

Research and development of LP EGR system for the performance improvement and emission reduction on diesel engine is proceeding at a good pace. LP EGR system seems to be helpful method to further reduce$NO_x$ emissions while maintaining PM emissions at a low level because the boost pressure is unchanged while varying EGR rate. This study is experimentally conducted on a 2.0L common rail DI engine at the medium load condition (2000 rpm, BMEP 1.0 MPa, boost pressure 181.3 kPa) that difficult to use large amount of EGR gas because of deteriorations of performance and fuel consumption. And we investigated the characteristics of performance and fuel consumption while varying EGR systems. The overall results using LP EGR system equipped with ETC identified benefits on reduction of PM and improvement of fuel consumption and thermal efficiency while keep the $NO_x$ level compared to HP EGR and LP EGR with back pressure valve.

• Journal of Drive and Control
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• v.9 no.4
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• pp.52-57
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• 2012

In this study, in order to understand contents and ranges of design for the EGR Valve test system for improving quality and performance of EGR Valve, engine performance and exhaust gas characteristic of 3L-class diesel engine was analyzed. Experimental operation of engine performance test was performed with 50% engine load and 20% and 100% opening ratio of EGR Valve. From test of performance and exhaust gas characteristic of engine, torque output of engine and temperature and pressure of inlet and outlet of EGR Valve were measured. As a result, for design of EGR Valve test system, input fluid flow of EGR Valve must be set the same amount with exhaust gas flow that was below of engine speed of 2,500 rpm, and temperature of inlet of EGR Valve must be set under about $510^{\circ}C$. And the difference of temperature between inlet and outlet of EGR Valve must be over than about $200^{\circ}C$. Exhaust gas of inlet and outlet of EGR Valve were under 1 bar that was not considerable, and the difference of pressure between inlet and outlet of EGR Valve were under 1 bar that could not effect on mechanical operation of EGR Valve.

• Development and Reproduction
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• v.18 no.1
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• pp.1-11
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• 2014

Early growth response 1 (Egr1) is a zinc-finger transcription factor to direct second-wave gene expression leading to cell growth, differentiation and/or apoptosis. While it is well-known that Egr1 controls transcription of an array of targets in various cell types, downstream target gene(s) whose transcription is regulated by Egr1 in the uterus has not been identified yet. Thus, we have tried to identify a list of potential target genes of Egr1 in the uterus by performing multi-step in silico promoter analyses. Analyses of mRNA microarray data provided a cohort of genes (102 genes) which were differentially expressed (DEGs) in the uterus between Egr1(+/+) and Egr1(-/-) mice. In mice, the frequency of putative EGR1 binding sites (EBS) in the promoter of DEGs is significantly higher than that of randomly selected non-DEGs, although it is not correlated with expression levels of DEGs. Furthermore, EBS are considerably enriched within -500 bp of DEG's promoters. Comparative analyses for EBS of DEGs with the promoters of other species provided power to distinguish DEGs with higher probability as EGR1 direct target genes. Eleven EBS in the promoters of 9 genes among analyzed DEGs are conserved between various species including human. In conclusion, this study provides evidence that analyses of mRNA expression profiles followed by two-step in silico analyses could provide a list of putative Egr1 direct target genes in the uterus where any known direct target genes are yet reported for further functional studies.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.6
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• pp.781-787
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• 2017

Objective: The early growth response (Egr) family consists of four members (Egr1, Egr2, Egr3, and Egr4) that are zinc finger transcription factors. Among them, Egr3 is involved in transcriptional regulation of target genes during muscle spindle formation and neurite outgrowth. We previously showed that the immunoreactive Egr3 is localized on oocyte spindle and accumulate near the microtubule organizing center during meiosis I in mice. Egr3 was also shown to be localized on spermatocytes. We herein investigated if Egr3 is expressed in mouse gonads and if Egr3 blockade results in any defect in oocyte maturation. Methods: Expression of Egr3 in mouse gonads was examined by reverse transcription-polymerase chain reaction. Full-length Egr3 and truncated Egr3 (${\Delta}Egr3$) complementary RNAs (cRNAs) with Xpress tag at N-terminus and DsRed2 at C-terminus, and small interfering RNA (siRNA) targeting Egr3 were microinjected into mouse oocytes at germinal vesicle stage. Localization of microinjected Egr3 was examined by confocal live imaging and immunofluorescence staining. Results: Egr3 mRNA was detected in mouse ovaries and testes from 1 to 4 week-old mice. An uncharacterized longer transcript containing 5'untranslated region was also detected in 3 and 4 week-old gonads. Microinjected Xpress-Egr3-DsRed2 or Xpress-${\Delta}Egr3$-DsRed2 localized to nuclei and chromosomes during meiotic progression. Microinjection of these cRNAs or Egr3 siRNA in oocytes did not affect meiotic maturation. Immunofluorescence staining of Egr3 in Xpress-${\Delta}Egr3$-DsRed2-injected oocytes showed a positive signal only on meiotic spindle, suggesting that this antibody does not detect endogenous or exogenous Egr3 in mouse oocytes. Conclusion: The results show that Egr3 localizes to chromosomes during meiotic progression and that certain antibodies may not faithfully represent localization of target proteins in oocytes. Egr3 seems to be dispensable during oocyte maturation in mice.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.10
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• pp.879-886
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• 2013

This study aims to provide helpful suggestions for understanding the effect of high EGR on DME HCCI combustion. This study determined which between oxygen partial pressure and oxygen concentration was the main factor affecting the LTHR heating ratio. Furthermore, EGR and the supercharging effect were investigated. To define the parameters for the EGR ratio and supercharging pressure, a numerical analysis of the chemical reaction was conducted under the following conditions: (1) variation of EGR ratio, oxygen concentration, and oxygen content; (2) variation of oxygen partial pressure while the oxygen concentration was almost constant; and (3) variation of oxygen concentration while oxygen partial pressure was constant with EGR and supercharging. The results show that an increase in EGR reduces the combustion duration. On the other hand, an increase in boost pressure increases the combustion duration. Finally, the EGR and boost pressure affect the amount of increase in LTHR.