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Inhibitory Effects of Rice Bran Water Extract Fermented Lactobacillus plantarum due to cAMP-dependent Phosphorylation of VASP (Ser157) on human Platelet Aggregation

  • Kim, Hyun-Hong (Department of Biomedical Laboratory Science, Inje University) ;
  • Lee, Dong-Ha (Department of Biomedical Laboratory Science, Inje University) ;
  • Hong, Jeong Hwa (Department of Smart Foods and Drugs, Inje University) ;
  • Ingkasupart, Pajaree (Department of Smart Foods and Drugs, Inje University) ;
  • Nam, Gi Suk (Department of Biomedical Laboratory Science, Inje University) ;
  • Ok, Woo Jeong (Department of Biomedical Laboratory Science, Inje University) ;
  • Kim, Min Ji (Department of Biomedical Laboratory Science, Inje University) ;
  • Yu, Young-Bin (Department of Biomedical Laboratory Science, College of Medical Science, Konyang University) ;
  • Kang, Hyo-Chan (Department of Medical Laboratory Science, Dong-eui Institute of Technology) ;
  • Park, Hwa-Jin (Department of Biomedical Laboratory Science, Inje University)
  • Received : 2015.04.22
  • Accepted : 2015.06.22
  • Published : 2015.06.30

Abstract

In this study, we investigated the effect of rice bran water extract fermented with Lactobacillus plantarum KCCM-12116 (RBLp) on ADP ($20{\mu}M$)-, collagen ($10{\mu}g/mL$)-, and thrombin (0.2 U/mL)-stimulated platelet aggregation. RBLp dose-dependently inhibited ADP-, collagen-, and thrombin-induced platelet aggregation, with $IC_{50}$ values of 501.1, 637.2, and > $2,000{\mu}g/mL$, respectively. The platelet aggregation induced by ADP plus RBLp ($750{\mu}g/mL$) was increased by the adenylate cyclase inhibitor, SQ22536, and the cAMP-dependent protein kinase (A-kinase) inhibitor, Rp-8-Br-cAMPS. Treatment with RBLp increased the phosphorylation of VASP ($Ser^{157}$), an A-kinase substrate, which was also inhibited by SQ22536 and Rp-8-Br-cAMPS. It is thought that the RBLp-induced increases in cAMP contributed to the phosphorylation of VASP ($Ser^{157}$), which in turn resulted in an inhibition of ADP-induced platelet aggregation, thereby indicating that RBLp has an antiplatelet effect via cAMP-dependent phosphorylation of VASP ($Ser^{157}$). Thus, RBLp may have therapeutic potential for the treatment (or prevention) of platelet aggregation-mediated diseases, such as thrombosis, myocardial infarction, atherosclerosis, and ischemic cerebrovascular disease.

Keywords

References

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