Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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A Simple Method for Evaluation of Pepper Powder Color Using Vis/NIR Hyperspectral System

Vis/NIR 초분광 분석을 이용한 고춧가루 색도 간이 측정법 개발

  • Han, Koeun (Department of Plant Science, Plant Genomics and Breeding Institute, and Vegetable Breeding Research Center, College of Agriculture and Life Sciences, Seoul National University) ;
  • Lee, Hoonsoo (Department of Biosystems Machinery Engineering, Chungnam National University) ;
  • Kang, Jin-Ho (Department of Plant Science, Plant Genomics and Breeding Institute, and Vegetable Breeding Research Center, College of Agriculture and Life Sciences, Seoul National University) ;
  • Choi, Eunah (Department of Plant Science, Plant Genomics and Breeding Institute, and Vegetable Breeding Research Center, College of Agriculture and Life Sciences, Seoul National University) ;
  • Oh, Se-Jeong (Hana Seed Ltd.) ;
  • Lee, Yong-Jik (Hana Seed Ltd.) ;
  • Cho, Byoung-Kwan (Department of Biosystems Machinery Engineering, Chungnam National University) ;
  • Kang, Byoung-Cheorl (Department of Plant Science, Plant Genomics and Breeding Institute, and Vegetable Breeding Research Center, College of Agriculture and Life Sciences, Seoul National University)
  • 한고은 (서울대학교 농업생명과학대학 식물생산과학부) ;
  • 이훈수 (충남대학교 바이오시스템기계공학과) ;
  • 강진호 (서울대학교 농업생명과학대학 식물생산과학부) ;
  • 최은아 (서울대학교 농업생명과학대학 식물생산과학부) ;
  • 오세정 (농업회사법인 (주)하나종묘) ;
  • 이용직 (농업회사법인 (주)하나종묘) ;
  • 조병관 (충남대학교 바이오시스템기계공학과) ;
  • 강병철 (서울대학교 농업생명과학대학 식물생산과학부)
  • Received : 2014.10.26
  • Accepted : 2015.01.29
  • Published : 2015.06.30
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Abstract

Color is one of the quality determining factors for pepper powder. To measure the color of pepper powder, several methods including high-performance liquid chromatography (HPLC), thin layer chromatography (TLC), and ASTA-20 have been used. Among the methods, the ASTA-20 method is most widely used for color measurement of a large number of samples because of its simplicity and accuracy. However it requires time consuming preprocessing steps and generates chemical waste containing acetone. As an alternative, we developed a fast and simple method based on a visible/near infrared (Vis/NIR) hyperspectral method to measure the color of pepper powder. To evaluate correlation between the ASTA-20 and the visible/near infrared (Vis/NIR) hyperspectral methods, we first measured the color of a total of 488 pepper powder samples using the two methods. Then, a partial least squares (PLS) model was postulated using the color values of randomly selected 3 66 samples to predict ASTA values of unknown samples. When the ASTA values predicted by the PLS model were compared with those of the ASTA-20 method for 122 samples not used for model development, there was very high correlation between two methods ($R^2=0.88$) demonstrating reliability of Vis/NIR hyperspectral method. We believe that this simple and fast method is suitable for highthroughput screening of a large number of samples because this method does not require preprocessing steps required for the ASTA-20 method, and takes less than 30 min to measure the color of pepper powder.

색도는 고추의 품질을 결정하는 중요한 요인으로, 색도 측정을 위해 high-performance liquid chromatography(HPLC), thin layer chromatography(TLC), ASTA-20 방법 등이 활용되고 있다. 특히 ASTA-20 방법은 간단하고 정확하게 다수의 시료에 대한 색도 분석을 수행할 수 있다는 장점 덕분에 주로 사용된다. 하지만 전처리 과정에 시간이 많이 소요되고 아세톤과 같은 폐시약이 발생한다. 따라서 본 연구에서는 ASTA-20 방법을 대체하기 위하여 Vis/NIR 초분광 분석법을 활용한 빠른 색도 분석법을 개발하고자 하였다. ASTA-20 방법과 Vis/NIR 초분광 분석법의 상관관계를 분석하기 위하여 총 488점 고춧가루의 색도를 두 가지 방법으로 측정하였다. 이후 무작위로 선발한 366개의 시료를 이용하여 Vis/NIR 초분광 분석법으로 측정한 값으로부터 ASTA 값을 예측하는 부분최소자승법(PLS) 모델을 확립하였다. 모델 개발에 활용하지 않은 122개 시료의 ASTA 값을 PLS 모델을 이용하여 예측하고, ASTA-20 방법으로 측정한 값과 비교해본 결과, 매우 유의성 있는 상관관계($R^2=0.88$)를 나타내 Vis/NIR 초분광 분석법의 신뢰도를 확인할 수 있었다. 따라서 본 연구에서 개발한 간편하고 빠른 ASTA 값 측정 방법은 전처리 단계를 요구하지 않고, 30분 이내에 100개 시료에 대한 분석을 수행할 수 있어 다수의 고춧가루 색도를 빠르게 측정하는데 유용하게 사용될 것으로 기대한다.

Keywords

References

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