Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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An Aminopropyl Carbazole Derivative Induces Neurogenesis by Increasing Final Cell Division in Neural Stem Cells

  • Shin, Jae-Yeon (Laboratory of Molecular and Stem Cell Pharmacology, College of Pharmacy, Chung-Ang University) ;
  • Kong, Sun-Young (Laboratory of Molecular and Stem Cell Pharmacology, College of Pharmacy, Chung-Ang University) ;
  • Yoon, Hye Jin (Laboratory of Medicinal Chemistry, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Ann, Jihyae (Laboratory of Medicinal Chemistry, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Lee, Jeewoo (Laboratory of Medicinal Chemistry, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Kim, Hyun-Jung (Laboratory of Molecular and Stem Cell Pharmacology, College of Pharmacy, Chung-Ang University)
  • Received : 2015.01.28
  • Accepted : 2015.06.01
  • Published : 2015.07.01
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Abstract

P7C3 and its derivatives, 1-(3,6-dibromo-9H-carbazol-9-yl)-3-(p-tolylamino)propan-2-ol (1) and N-(3-(3,6-dibromo-9H-carbazol-9-yl)-2-hydroxypropyl)-N-(3-methoxyphenyl)-4-methylbenzenesulfonamide (2), were previously reported to increase neurogenesis in rat neural stem cells (NSCs). Although P7C3 is known to increase neurogenesis by protecting newborn neurons, it is not known whether its derivatives also have protective effects to increase neurogenesis. In the current study, we examined how 1 induces neurogenesis. The treatment of 1 in NSCs increased numbers of cells in the absence of epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF2), while not affecting those in the presence of growth factors. Compound 1 did not induce astrocytogenesis during NSC differentiation. 5-Bromo-2'-deoxyuridine (BrdU) pulsing experiments showed that 1 significantly enhanced BrdU-positive neurons. Taken together, our data suggest that 1 promotes neurogenesis by the induction of final cell division during NSC differentiation.

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References

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