KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Nutrient Limitation on Lipid Content and Fatty Acid Composition of Mutant Chlamydomonas reinhardtii

돌연변이 Chlamydomonas reinhardtii의 영양분 제한에 따른 지질 생산 및 지방산 조성 변화 연구

  • Baek, Jaewon (Department of Biotechnology and Bioengineering, Interdisciplinary Program of Bioenergy and Biomaterials, Chonnam National University) ;
  • Choi, Jong-il (Department of Biotechnology and Bioengineering, Interdisciplinary Program of Bioenergy and Biomaterials, Chonnam National University)
  • Received : 2015.02.13
  • Accepted : 2015.03.31
  • Published : 2015.04.27
Download PDF
⟨ Previous Next ⟩

Abstract

Production of biodiesel from microalgae is dependent on the microalgal lipid content and free fatty acid composition. Both lipid and free fatty acid are regulated by nutrient sources. In this study, newly developed mutant Chlamydomonas reinhardtii with higher lipid content was investigated for the effect of nutrient limitation. Nitrogen $NO_3{^{-}}$ and phosphate $PO_4{^{3-}}$ were limited for nutrient starvation during the cultivation. Under nutrient starvation, total lipid content level was increased to 27~33% and C16:0 fatty acid content constituted over 31~43% of total fatty acid. Interestingly, we also found that the expression of fatty acid desaturase (FAD7) was decreased when nutrients were starved.

Keywords

References

  1. Brennan, L. and P. Owende (2010) Biofuels from microalgae-A review of technologies for production, processing, and extractions of biofuels and co-products. Renewable and Sustainable Energy Reviews 14.2: 557-577. https://doi.org/10.1016/j.rser.2009.10.009
  2. Ahmad, A. L., N. H. Yasin, C. J. C. Derek, and J. K. Lim (2011) Microalgae as a sustainable energy source for biodiesel production. Renewable and Sustainable Energy Reviews 15: 584-593. https://doi.org/10.1016/j.rser.2010.09.018
  3. Scurlock, J. M. O., D. O. Hall, J. I. House, and R. Howes (1993) Utilizing biomass crops as an energy source. A European perspective. Springer Netherlands 70: 499-518. https://doi.org/10.1007/BF01105018
  4. Kosaric, N. and J. Velikonja (1995) Liquid and gaseous fuels from biotechnology: Challenge and opportunities. FEMS Microbiology Reviews 16: 111-142. https://doi.org/10.1111/j.1574-6976.1995.tb00161.x
  5. Duffy, J. E., E. A. Canuel, W. Adey and J. P. Swaddle (2009) Biofuels: Algae. Science 326: 1345.
  6. Brune, D. E., T. J. Lundquist, and J. R. Benemann (2009) Microalgal biomass for greenhouse gas reductions: potential for replacement of fossil fuels and animal feeds. Journal of Environmental Engineering 135: 1136-1144. https://doi.org/10.1061/(ASCE)EE.1943-7870.0000100
  7. Demirbas, A. (2007) Importance of biodiesel as transportation fuel. Energy Policy 35: 4661-4670. https://doi.org/10.1016/j.enpol.2007.04.003
  8. Guckert, J. B. and K. E. Cooksey (1990) Triglyceride accumulation and fatty acid profile changes in Chlorella (Chlorophyta) during high pH-induced cell cycle inhibition. Journal of Phycology 26: 72-79. https://doi.org/10.1111/j.0022-3646.1990.00072.x
  9. Wang, Z. T., N. Ullrich, S. Joo, S. Waffenschmidt, and U. Goodenough (2009) Algal lipid bodies: stress induction, purification, and biochemical characterization in wild type and starchless Chlamydomonas reinhardtii. Eukaryotic Cell 8: 1856-1868. https://doi.org/10.1128/EC.00272-09
  10. Grillitsh, K. and G. Daum (2011) Triacylglycerol lipases of the yeast. Frontiers in Biology 6: 219-230.
  11. Choi, J., M., Yoon, and D.H. Kim (2014) A mutant Chlamydomonas reinhardtii M4013 having higher lipid content and preparation method thereof, and the production of bio-diesel from the mutant Chlamydomonas reinhardtii M4013. Korea Patent 10-2013-0041784.
  12. Bischoff, H. W. and H. C. Bold (1963). Some soil algae from Enchanted Rock and related algal species (No. 4). University of Texas.
  13. Yao, S., A. Brandt, H. Egsgaard, and C. Gjermansen (2012) Neutral lipid accumulation at elevated temperature in conditional mutants of two microalgae species. Plant Physiology and Biochemistry 61: 71-79. https://doi.org/10.1016/j.plaphy.2012.09.007
  14. Solovchenko, A. E., I. Khozin-Goldberg, S. Didi-Cohen, Z. Cohen, and M. N. Merzlyak (2008) Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalga Parietochloris incisa. Journal of Applied Phycology 20: 245-251. https://doi.org/10.1007/s10811-007-9233-0
  15. Rodolfi, L., G. C. Zittelli, N. Bassi, G. Padovani, N. Biondi, G. Bonini, and M. R. Tredici (2009) Microalgae for Oil: Strain Selection, Induction of Lipid Synthesis and Outdoor Mass Cultivation in a Low-Cost Photobioreactor. Biotechnology and Bioengineering 102: 100-112. https://doi.org/10.1002/bit.22033
  16. Bilanovic, D., A. Andargatchew, T. Kroeger, and G. Shelef (2009) Freshwater and marine microalgae sequestering of $CO_2$ at different C and N concentrations - Response surface methodology analysis. Energy Conversion and Management 50: 262-267. https://doi.org/10.1016/j.enconman.2008.09.024
  17. Hu, G., Y. Fan, L. Zhang, C. Yuan, J. Wang, W. Li, and F. Li (2013) Enhanced lipid productivity and photosynthesis efficiency in a Desmodesmus sp. mutant induced by heavy carbon ions. PloS one. 8: e60700. https://doi.org/10.1371/journal.pone.0060700
  18. An, M., S. Mou, X. Zhang, Z. Zheng, N. Ye, D. Wang, and J. Miao (2013) Expression of fatty acid desaturase genes and fatty acid accumulation in Chlamydomonas sp. ICE-L under salt stress. Bioresource Technology 149: 77-83. https://doi.org/10.1016/j.biortech.2013.09.027