Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Propyl Gallate Inhibits Adipogenesis by Stimulating Extracellular Signal-Related Kinases in Human Adipose Tissue-Derived Mesenchymal Stem Cells

  • Lee, Jeung-Eun (School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology) ;
  • Kim, Jung-Min (School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology) ;
  • Jang, Hyun-Jun (School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology) ;
  • Lim, Se-Young (School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology) ;
  • Choi, Seon-Jeong (Department of Food Science and Technology, Korea National University of Transportation) ;
  • Lee, Nan-Hee (Department of Food Nutrition and Cook, Daegu Science University) ;
  • Suh, Pann-Ghill (School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology) ;
  • Choi, Ung-Kyu (Department of Food Science and Technology, Korea National University of Transportation)
  • Received : 2014.09.02
  • Accepted : 2014.12.24
  • Published : 2015.04.30
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Abstract

Propyl gallate (PG) used as an additive in various foods has antioxidant and anti-inflammatory effects. Although the functional roles of PG in various cell types are well characterized, it is unknown whether PG has effect on stem cell differentiation. In this study, we demonstrated that PG could inhibit adipogenic differentiation in human adipose tissue-derived mesenchymal stem cells (hAMSCs) by decreasing the accumulation of intracellular lipid droplets. In addition, PG significantly reduced the expression of adipocyte-specific markers including peroxisome proliferator-activated receptor-${\gamma}$ (PPAR-${\gamma}$), CCAAT enhancer binding protein-${\alpha}$ (C/EBP-${\alpha}$), lipoprotein lipase (LPL), and adipocyte fatty acid-binding protein 2 (aP2). PG inhibited adipogenesis in hAMSCs through extracellular regulated kinase (ERK) pathway. Decreased adipogenesis following PG treatment was recovered in response to ERK blocking. Taken together, these results suggest a novel effect of PG on adipocyte differentiation in hAMSCs, supporting a negative role of ERK1/2 pathway in adipogenic differentiation.

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References

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