Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Investigation of Microsatellite Markers for Traceability and Individual Discrimination of Korean Native Ducks

한국 토종오리의 개체 식별 및 품종 구분을 위한 Microsatellite 마커 탐색

  • Seo, Dong Won (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Sultana, Hasina (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Choi, Nu Ri (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Yeon Su (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Jin, Shil (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Heo, Kang Nyeong (Poultry Science Division, National Institute of Animal Science, RDA) ;
  • Jin, Seon Deok (Natural History Research Team, National Science Museum) ;
  • Lee, Jun Heon (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
  • 서동원 (충남대학교 동물자원생명과학과) ;
  • 술타나 (충남대학교 동물자원생명과학과) ;
  • 최누리 (충남대학교 동물자원생명과학과) ;
  • 김연수 (충남대학교 동물자원생명과학과) ;
  • 진실 (충남대학교 동물자원생명과학과) ;
  • 허강녕 (농촌진흥청 국립축산과학원 가금과) ;
  • 진선덕 (국립중앙과학관 자연사연구팀) ;
  • 이준헌 (충남대학교 동물자원생명과학과)
  • Received : 2014.10.29
  • Accepted : 2015.03.12
  • Published : 2015.03.31
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Abstract

Recently, duck meat consumption has been rapidly increased because consumers recognized duck meat for healthy food. In relation to this, Korean duck industry need to develop Korean native duck (KND) breed for both conservation perspective and self-sufficient of the breeding stocks. In this study, 24 microsatellite (MS) markers were investigated for classification of KND and commercial duck (CD) breeds in the Korean market. Using these MS markers, the calculated number of alleles (K), expected heterozygosity (He) values and polymorphic information contents (PIC) were 1~16, 0~0.865 and 0~0.841, respectively. Also, the expected probability of identical values in random individuals (PI), random sib ($PI_{sib}$) and random half-sib ($PI_{half-sib}$) were estimated as $1.64{\times}10^{-16}$, $2.60{\times}10^{-7}$ and $1.30{\times}10^{-12}$, respectively. The results indicated that the expected probabilities of identity powers were enough for the individual identification. However, KND and CD breeds were not fully discriminated well using the 24 MS markers, which may CD and KND has shared same origin or crossbred. Therefore, further studies will be ultimately needed for developing a genetically pure line of KND breed even though the DNA markers used. Finally, these results will provide useful information for individual traceability system in ducks.

최근, 한국의 소비자들이 건강에 대한 관심이 증가하면서 단일불포화 지방산이 풍부해 건강에 긍정적인 영향을 줄 수 있는 오리고기의 수요가 급격하게 증가하고 있다. 하지만 대부분의 종 오리는 수입에 의존하고 있는 실정이기 때문에, 토종오리의 개발 및 보급이 필요한 실정이며, 이는 종자주권의 확립 및 농가 소득 증대에도 매우 필요한 일이라 할 수 있다. 따라서 본 연구에서는 24개의 microsatellite 마커를 확보하였으며, 이들 마커의 대립유전자수는 1~16개, 이형접합도는 0~0.865, 다형성은 0~0.841로 확인되었다. 이들 마커를 이용하여 임의 집단에서 동일개체 출현빈도를 계산한 결과는 임의 집단 $1.64{\times}10^{-16}$, 전형매 집단 $2.60{\times}10^{-7}$, 반형매 집단 $1.30{\times}10^{-12}$으로 높은 개체식별률과 친자확인도를 확인할 수 있었다. 하지만 이들 마커를 이용한 계통분석 결과, 토종오리와 실용오리 집단을 정확하게 구분하기에는 어려운 것으로 확인되었다. 따라서 추가연구를 통해 토종오리의 순종화 및 더 정확한 토종오리와 실용오리 집단 구분이 가능한 마커 개발이 필요할 것으로 사료된다.

Keywords

References

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