Biomaterials and Biomechanics in Bioengineering

  • 제2권1호
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  • Pages.33-45
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  • 2015
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  • 2465-9835(pISSN)
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  • 2465-9959(eISSN)
테크노프레스 (Techno-Press)
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DOI QR코드

DOI QR Code

Fabrication of multi-well platform with electrical stimulation for efficient myogenic commitment of C2C12 cells

  • Song, Joohyun (School of Chemical and Biological Engineering, Seoul National University) ;
  • Lee, Eunjee A. (School of Chemical and Biological Engineering, Seoul National University) ;
  • Cha, Seungwoo (School of Chemical and Biological Engineering, Seoul National University) ;
  • Kim, Insun (School of Chemical and Biological Engineering, Seoul National University) ;
  • Choi, Yonghoon (School of Chemical and Biological Engineering, Seoul National University) ;
  • Hwang, Nathaniel S. (School of Chemical and Biological Engineering, Seoul National University)
  • 투고 : 2015.02.12
  • 심사 : 2015.02.25
  • 발행 : 2015.03.25
⟨ 이전 논문 다음 논문 ⟩

초록

To engineer tissue-like structures, cells are required to organize themselves into three-dimensional networks that mimic the native tissue micro-architecture. Here, we present agarose-based multi-well platform incorporated with electrical stimulation to build skeletal muscle-like tissues in a facile and highly reproducible fashion. Electrical stimulation of C2C12 cells encapsulated in collagen/matrigel hydrogels facilitated the formation 3D muscle tissues. Consequently, we confirmed the transcriptional upregulations of myogenic related genes in the electrical stimulation group compared to non-stimulated control group in our multi-well 3D culture platform. Given the robust fabrication, engineered muscle tissues in multi-well platform may find their use in high-throughput biological studies drug screenings.

키워드

과제정보

연구 과제 주관 기관 : National Research Foundation of Korea (NRF)

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