Biomaterials and Biomechanics in Bioengineering

  • 제2권1호
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  • Pages.23-32
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  • 2015
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  • 2465-9835(pISSN)
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  • 2465-9959(eISSN)
테크노프레스 (Techno-Press)
권호 표지
DOI QR코드

DOI QR Code

Injectable TGF-beta 3-conjugated hyaluronic acid hydrogel for cartilage regeneration

  • Ko, Ki Seong (Department of Molecular Science and Technology, Ajou University) ;
  • Lee, Jung Seok (Department of Molecular Science and Technology, Ajou University) ;
  • Park, Kyung Min (Division of Bioengineering, Incheon National University) ;
  • Lee, Yunki (Department of Molecular Science and Technology, Ajou University) ;
  • Oh, Dong Hwan (Department of Molecular Science and Technology, Ajou University) ;
  • Son, Joo Young (Department of Molecular Science and Technology, Ajou University) ;
  • Kwon, Oh Hee (Deparment of Biomedical Engineering, Ajou University) ;
  • Eom, Min Yong (Department of Molecular Science and Technology, Ajou University) ;
  • Park, Ki Dong (Department of Molecular Science and Technology, Ajou University)
  • 투고 : 2015.01.03
  • 심사 : 2015.02.12
  • 발행 : 2015.03.25
⟨ 이전 논문 다음 논문 ⟩

초록

Facile immobilization of growth factors in hyaluronic acid (HA) hydrogels using dual enzymes is reported in the paper. The hydrogels were formed by using horseradish peroxidase (HRP) and hydrogen peroxide ($H_2O_2$) and transforming growth factor-${\beta}3$ (TGF-${\beta}3$) was covalently conjugated on the hydrogels in situ using tyrosinase (Ty) without any modifications. For the preparation of hydrogels, HA was grafted with poly(ethylene glycol) (PEG), which was modified with a tyrosine. The gelation times of the HA hydrogels were ranging from 415 to 17 s and the storage moduli was dependent on the concentration of $H_2O_2$ and Ty (470-1600 Pa). A native TGF-${\beta}3$ (200 ng/mL) was readily encapsulated in the HA hydrogels and 17% of the TGF-${\beta}3$ was released over 1 month at the Ty concentration of 0.5 KU/mL, while the release was faster when 0.3 KU/mL of Ty was used for the encapsulation (27%). It can be suggested that the growth factors resident in the hydrogels for a long period of time may lead cells proliferating and differentiating, whereas the growth factors that are initially released from the hydrogels can induce the ingrowth of cells into the matrices. Therefore, the dual enzymatic methods as facile gel forming and loading of various native growth factors or therapeutic proteins could be highly promising for tissue regenerative medicines.

키워드

과제정보

연구 과제 주관 기관 : National Research Foundation of Korea (NRF)

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피인용 문헌

  1. Peroxidase-catalyzed microextrusion bioprinting of cell-laden hydrogel constructs in vaporized ppm-level hydrogen peroxide vol.10, pp.4, 2018, https://doi.org/10.1088/1758-5090/aadc9e