The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Gibberellins Production and Identification of Endophytic Fungi Isolated from Aquatic Plant in Fresh Water

담수에 자생하는 수생식물에서 분리된 내생균류의 지베렐린 생산과 동정

  • You, Young-Hyun (School of Life Science, Kyungpook National University) ;
  • Kang, Sang-Mo (School of Applied Biosciences, Kyungpook National University) ;
  • Choi, Yu-Mi (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Lee, Myung-Chul (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Kim, Jong-Guk (School of Life Science, Kyungpook National University)
  • 유영현 (경북대학교 생명과학부) ;
  • 강상모 (경북대학교 응용생명과학부) ;
  • 최유미 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이명철 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 김종국 (경북대학교 생명과학부)
  • Received : 2015.01.24
  • Accepted : 2015.02.03
  • Published : 2015.03.31
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Abstract

Aquatic plant Hydrocharis dubia (Blume) Backer was collected from the Dalsung wetland in Daegu. Sixteen endophytic fungi with different colony morphologies were isolated from the roots of aquatic plants. Waito-c rice (WR) seedlings were treated with fungal culture filtrates (FCF) for screening plant growth-promoting activity. In the results, HD1008 strain isolated from aquatic plant showed highest plant growth-promoting activity. The FCF of HD1008 strain was analyzed using gas chromatography mass spectrometry (GC/MS) with selected ion monitoring (SIM). Analysis of the FCF of HD1008 strain found that it contained gibberellins (GA) ($GA_1$, 1.2 ng/100 mL; $GA_4$, 5 ng/100 mL). Phylogenetic tree of HD1008 strain was constructed by partial internal transcribed spacer (ITS) region and partial beta-tubulin gene sequences. Therefore, we describe HD1008 strain as a new gibberellin-producing Penicillium trzebinskii based on morphological and molecular characteristics.

수생식물 샘플은 낙동강과 금호강이 만나는 달성습지에서 자라풀을 채집하였다. 자라풀의 뿌리에서 내생균류를 분리하고 형태가 다른 균주를 관찰하여 최종적으로 16개 균주를 선발하였다. 내생균류의 배양여과액은 식물생장촉진활성 검정을 위하여 난장이벼에 처리하여 스크리닝하였으며, HD1008 균주가 식물생장촉진활성이 가장 높은 것으로 확인되었다. HD1008 균주의 배양여과액을 HPLC와 GC/MS-SIM을 이용하여 분석하였고, HD1008 균주가 식물호르몬인 지베렐린 $GA_1$ (1.2 ng/100 mL)과 $GA_4$ (5 ng/100 mL) 를 생산하는 것을 정량분석을 통하여 확인하였다. 또한, HD1008균주의 beta-tubulin 유전자 염기서열을 이용하여 동정에 이용하였으며, 분자적인 방법과 형태적인 방법으로 관찰하였을 때, 지베렐린을 생산하는 새로운 P. trzebinskii로 동정되었다.

Keywords

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