Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Expression of IER3 in Primary Hepatocarcinoma: Correlation with Clinicopathological Parameters

  • Liu, Zhong (Medical College, Qingdao University) ;
  • Wang, Xin-Mei (Department of Chemotherapy, Department of Pathology, Zibo Center Hospital) ;
  • Jia, Tong-Fu (Department of Chemotherapy, Department of Pathology, Zibo Center Hospital) ;
  • Zhai, Yi (Department of Chemotherapy, Department of Pathology, Zibo Center Hospital) ;
  • Sun, Ling-Yan (Department of Chemotherapy, Department of Pathology, Zibo Center Hospital) ;
  • Cheng, Yu-Ping (Department of Chemotherapy, Department of Pathology, Zibo Center Hospital) ;
  • Zhang, Yue-Min (Department of Chemotherapy, Department of Pathology, Zibo Center Hospital) ;
  • Liu, Shi-Hai (Department of Medical Research Center, Department of Oncology, the Affiliated Hospital of Qingdao University) ;
  • Liang, Jun (Department of Medical Research Center, Department of Oncology, the Affiliated Hospital of Qingdao University)
  • Published : 2015.02.25
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Abstract

Background: Studies indicate the immediate early response gene 3 (IER3) is involved in many biological processes. Recently, it was discovered that IER3 plays an important role in tumorigenesis and tumor progression. Thus it may be a valuable biomarker in tumor. This study was designed to investigate the expression status of IER3 in primary hepatocarcinoma (PHC) and correlation with clinicopathological parameters. Materials and Methods: Real-time PCR was performed to evaluate the expression levels of IER3 in 62 pathologically diagnosed human PHC specimens. Results: A statistically significant association was disclosed between the expression of IER3 and P53 mutant protein (short for P53), Ki-67, EGFR and the biggest diameter, differentiation grade of tumor. Conclusions: This work is the first to shed light on the potential clinical usefulness of IER3, as an efficient tumor biomarker in PHC.

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References

  1. Akilov OE, Wu MX, Ustyugova IV, Falo LD Jr, Geskin LJ, (2012). Resistance of Sezary cells to TNF-$\alpha$- induced apoptosis is mediated in part by a loss of TNFRl and a high level of the IER3 expression. Exp Dermatol, 21, 287-92. https://doi.org/10.1111/j.1600-0625.2012.01452.x
  2. Arlt A, Grobe O, Sieke A, et al (2001). Expression of the NFkappa B target gene IEX-1 (p22/PRG1) does not prevent cell death but instead triggers apoptosis in Hela cells. Oncogene, 20, 69-76. https://doi.org/10.1038/sj.onc.1204061
  3. Arlt A, Kruse ML, Breitenbroich M, et al (2003). The early response gene IEX-1 attenuates NF-kappaB activation in 293 cells, a possible counter-regulatory process leading to enhanced cell death. Oncogene, 22, 3343-51. https://doi.org/10.1038/sj.onc.1206524
  4. Bruheim S, Xi Y, Ju J, Fodstad O, (2009). Gene expression profiles classify human osteosarcoma xenografts according to sensitivity to doxorubicin, cisplatin and ifosfamide. Clin Cancer Res, 15, 7161-9. https://doi.org/10.1158/1078-0432.CCR-08-2816
  5. Charles CH, Yoon JK, Simske JS, Lau LF, (1993). Genomic structure, cDNA sequence and expression of gly96, a growth factor-inducible immediate-early gene encoding a short-lived glycosylated protein. Oncogene, 8, 797-801.
  6. Dilley WG, Kalyanaraman S, Verma S, et al (2005). Global gene expression in neuroendocrine tumors from patients with the MEN1 syndrome. Mol Cancer, 4, 9. https://doi.org/10.1186/1476-4598-4-9
  7. Feldmann KA, Pittelkow MR, Roche PC, et al (2001). Expression of an immediate early gene, IEX-1, in human tissues. Histochem Cell Biol, 115, 489-97.
  8. Hamidi T, Algul H, Cano CE, et al (2012). Nuclear protein 1 promotes pancreatic cancer development and protects cells from stress by inhibiting apoptosis. J Clin Invest, 122, 2092-103. https://doi.org/10.1172/JCI60144
  9. Han L, Geng L, Liu X, et al (2011). Clinical significance of expression in ovarian carcinoma. Ultrastruct Pathol, 35, 260-6. https://doi.org/10.3109/01913123.2011.608916
  10. Hu Y, Sun H, Drake J, et al (2004). From mice to humans: identification of commonly deregulated genes in mammary cancer via comparative SAGE studies. Cancer Res, 64, 7748-55. https://doi.org/10.1158/0008-5472.CAN-04-1827
  11. Huang YH, Wu JY, Zhang Y, Wu MX, (2002). Synergistic and opposing regulation of the stress-responsive gene IEX-1 by p53, c-Myc and multiple NF-${\kappa}B$/rel complexes. Oncogene, 21, 6819-28. https://doi.org/10.1038/sj.onc.1205854
  12. Ishimoto Y, Satsu H, Totsuka M, Shimizu M, (2011). IEX-1 suppressed apoptotic damage in human intestinal epithelial Caco-2 cells induced by coculturing with macrophage-like THP-1 cells. Biosci Rep, 31, 345-51. https://doi.org/10.1042/BSR20100083
  13. Kondratyev AD, Chung KN, Jung MO, (1996). Identification and characterization of a radiation-inducible glycosylated human early-response gene. Cancer Res, 56, 1498-502.
  14. Nambiar PR, Nakanishi M, Gupta R, et al (2004). Genetic signatures of high- and low-risk aberrant crypt foci in a mouse model of sporadic colon cancer. Cancer Res, 64, 6394-401. https://doi.org/10.1158/0008-5472.CAN-04-0933
  15. Osawa Y, Nagaki M, Banno Y, et al (2003). Expression of the NF-kappa B target gene X-ray-inducible immediate early response factor-1 short enhances TNF-alpha-induced hepatocyte apoptosis by inhibiting Akt activation. J Immunol, 170, 4053-60. https://doi.org/10.4049/jimmunol.170.8.4053
  16. Sasada T, Azuma K, Hirai T, et al (2008). Prognostic significance of the immediate early response gene X-1 (IEX-1) expression in pancreatic cancer. Ann Surg Oncol, 15, 609-17. https://doi.org/10.1245/s10434-007-9669-0
  17. Schilling D, Pittelkow MR, Kumar R, (2001). IEX-1, an immediate early gene, increases the rate of apoptosis in keratinocytes. Oncogene, 20, 7992-7. https://doi.org/10.1038/sj.onc.1204965
  18. Semlali A, Oliva J, Badia E, Pons M, Duchesne MJ, (2004). Immediate early gene X-1 (IEX-1), a hydroxytamoxifen regulated gene with increased stimulation in MCF-7 derived resistant breast cancer cells. J Steroid Biochem Mol Biol, 88, 247-59. https://doi.org/10.1016/j.jsbmb.2003.12.005
  19. Wu MX (2008). Roles of the stress-induced gene IEX-1 in regulation of cell death and oncogenesis. Apoptosis, 8, 11-8.
  20. Xiao T, Zhu W, Yao MJ, et al (2008). Study on the mechanism of transcriptional regulation of IEX-1 gene induced by AS2O3 in human osteosarcoma MG-63. Progress Biochem Biophys, 35, 828-33.
  21. Yang C, Trent S, Ionescu-Tiba V, et al (2006). Identification of cyclin D1- and estrogen-regulated genes contributing to breast carcinogenesis and progression. Cancer Res, 66, 11649-58. https://doi.org/10.1158/0008-5472.CAN-06-1645
  22. Zhang Y, Schlossman SF, Edwards RA, et al (2002). Impaired apoptosis, extended duration of immune responses and a lupus-like autoimmune disease in IEX-1-trans-genic mice. Proc Natl Acad Sci USA, 99, 878-83. https://doi.org/10.1073/pnas.022326699
  23. Zhang Y, Finegold MJ, Porteu F, Kanteti P, Wu MX, (2003). Development of T-cell lymphomas in Emu-IEX-1 mice. Oncogene, 22, 6845-51. https://doi.org/10.1038/sj.onc.1206707