Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Anti-Ionizing Radiation Effect of Selenium on Osteoporosis Model during Bone Repair Process

골다공증 모델의 뼈 재생기에 있어 셀레늄(Selenium)의 방사선 보호작용

  • Kim, Hyun (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Cho, Sang-Rae (Hanwoo Research Station, National Institute of Animal Science, RDA) ;
  • Choe, Changyong (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Seong, Hwan-Hoo (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
  • 김현 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 조상래 (농촌진흥청 국립축산과학원 한우연구소) ;
  • 최창용 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 성환후 (농촌진흥청 국립축산과학원 가축유전자원센터)
  • Received : 2015.01.30
  • Accepted : 2015.09.22
  • Published : 2015.09.30
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Abstract

Selenium (Se) is an essential trace element for humans and animals, and several findings suggest that dietary Se intake may be necessary for bone health. Accumulating evidence indicates that Se compounds possess anticancer properties. Se is specifically incorporated into proteins in the form of selenocysteine and non-specifically incorporated as selenomethionine in place of methionine. This study evaluated protection by Se in the bone repair process in ovariectomized rats after irradiation. For such purpose, 80 ovariectomized female Sprague-Dawley rats were randomly divided into 4 experimental groups: ovariectomized (Ov), Ov/Se, Ov/irradiated (Irr) and Ov/ Se/Irr. A bone defect was created on the tibia of all animals 40 days after ovariectomy. Two days after surgery, only the Ov/Se and Ov/Se/Irr rats received 0.8 mg Se/kg. Three days after surgery, only the Ov/Irr and Ov/Se/Irr rats received 10 Gy of X-rays on the lower limb region. The animals were euthanized at 7, 15, 22 and 29 days after surgery to assess the repair process, which was evaluated by analysis of trabecular bone number (Masson Trichrome) and birefringence analysis (Picrosirius). It was possible to observe a delay in the bone repair process in the ovariectomized/irradiated group and similarity between the ovariectomized, Ov/ Se and Ov/Se/Irr groups. Our findings suggest that sodium selenite may influence a radioprotective effect in the bone repair of tibia of ovariectomized rats without toxicity.

본 연구는 방사선 조사 후, 난소 적출을 한 흰쥐에 있어서 골회복과정 중에 셀레늄(Se)에 의한 보호 작용을 평가한 연구이다. 목적 수행을 위해 난소 적출술을 실시한 암컷 Sprague-Dawley계 흰쥐 80마리를 무작위로 ovariectomized(Ov), Ov/Se, Ov/irradiated(Irr) 그리고 Ov/Se/Irr와 같이 4개의 처리군으로 구분하였다. 골 결손은 난소적출(ovariectomy) 후, 40일에 모든 공시동물의 경골(tibia)에서 만들어졌다. 수술 후, 48시간에 Ov/Se군과 Ov/Se/Irr 처리구의 흰쥐에만 0.8 mg Se/kg을 투여하였다. 수술 후, 72시간에 Ov/Irr 군과 Ov/Se/Irr 처리구의 흰쥐에만 10Gy의 X-rays 조사를 사지부위(limb region)에 실시하였다. 수술 후, 모든 공시동물은 7, 15, 22 그리고 29일에 안락사를 시키고, 섬유주골형(trabecular bone)의 개수 분석(Masson Trichrome) 과 복 굴절 분석(Picrosirius)에 의해서 골 회복과정을 평가하였다. 난소적출한 군 및 방사선 조사한 군에서 골 재생 과정이 지연되는 현상을 확인이 가능하였다. 결론적으로, 셀렌산염 (sodium selenite)은 독성 없이 난소 적출을 한 흰쥐의 경골(tibia)의 골 재생과정에서 방사선 보호작용을 할 가능성을 제시하였다.

Keywords

References

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