Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Melanin Biosynthesis Inhibition Effects of Ginsenoside Rb2 Isolated from Panax ginseng Berry

  • Lee, Dae Young (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Jeong, Yong Tae (HK BIO, Business Incubator, Daegu Haany University) ;
  • Jeong, Sang Chul (Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resources) ;
  • Lee, Mi Kyoung (Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyung Hee University) ;
  • Min, Jin Woo (Research & Development Center, GFC Co., Ltd.) ;
  • Lee, Jae Won (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Geum Soog (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Seung Eun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Ahn, Young Sup (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kang, Hee Cheol (Research & Development Center, GFC Co., Ltd.) ;
  • Kim, Jin Hee (College of Herbal Bio-industry, Daegu Haany University)
  • Received : 2015.05.22
  • Accepted : 2015.10.04
  • Published : 2015.12.28
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Abstract

Ginsenoside Rb2 (Gin-Rb2) was purified from the fruit extract of Panax ginseng. Its chemical structure was measured by spectroscopic analysis, including HR-FAB-MS, 1H-NMR, and IR spectroscopy. Gin-Rb2 decreased potent melanogenesis in melan-a cells, with 23.4% at 80 μM without cytotoxicity. Gin-Rb2 also decreased tyrosinase and MITF protein expression in melan-a cells. Furthermore, Gin-Rb2 presented inhibition of the body pigmentation in the zebrafish in vivo system and reduced melanin contents and tyrosinase activity. These results show that Gin-Rb2 isolated from P. ginseng may be an effective skin-whitening agent via the in vitro and in vivo systems.

Keywords

References

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