Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase

  • Cho, Jin-Beom (Department of Bio and Nanochemistry, Kookmin University) ;
  • Lee, Jin-Moo (Department of Bio and Nanochemistry, Kookmin University) ;
  • Ahn, Hee-Chul (Department of Pharmacy, Dongguk University) ;
  • Jeong, Yong-Joo (Department of Bio and Nanochemistry, Kookmin University)
  • Received : 2015.07.22
  • Accepted : 2015.09.15
  • Published : 2015.12.28
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Abstract

A new chemical inhibitor against severe acute respiratory syndrome (SARS) coronavirus helicase, 7-ethyl-8-mercapto-3-methyl-3,7-dihydro-1H-purine-2,6-dione, was identified. We investigated the inhibitory effect of the compound by conducting colorimetry-based ATP hydrolysis assay and fluorescence resonance energy transfer-based double-stranded DNA unwinding assay. The compound suppressed both ATP hydrolysis and double-stranded DNA unwinding activities of helicase with IC50 values of 8.66 ± 0.26 μM and 41.6 ± 2.3 μM, respectively. Moreover, we observed that the compound did not show cytotoxicity up to 80 μM concentration. Our results suggest that the compound might serve as a SARS coronavirus inhibitor.

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References

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