Journal of Plant Biotechnology

  • 제42권3호
  • /
  • Pages.204-214
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  • 2015
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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배추 SHI-RELATED SEQUENCE 유전자 발현이 페튜니아 생장 발달에 미치는 영향

Effects of Brassica rapa SHI-RELATED SEQUENCE overexpression on petunia growth and development

  • 홍준기 (농촌진흥청 국립농업과학원) ;
  • 서은정 (농촌진흥청 국립농업과학원) ;
  • 이수영 (농촌진흥청 국립원예특작과학원 화훼과) ;
  • 송천영 (한국농수산대학 화훼학과) ;
  • 이승범 (농촌진흥청 국립농업과학원) ;
  • 김진아 (농촌진흥청 국립농업과학원) ;
  • 이수인 (농촌진흥청 국립농업과학원) ;
  • 이연희 (농촌진흥청 국립농업과학원)
  • Hong, Joon Ki (National Academy of Agricultural Science, Rural Development Administration) ;
  • Suh, Eun Jung (National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Su Young (Floricultural Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Song, Cheon Young (Department of Floriculture, Korea National College of Agriculture and Fisheries) ;
  • Lee, Seung Bum (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Jin A (National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Soo In (National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Yeon-Hee (National Academy of Agricultural Science, Rural Development Administration)
  • 투고 : 2015.06.29
  • 심사 : 2015.07.29
  • 발행 : 2015.09.30
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초록

SHI-RELATED SEQUENCE (SRS) 유전자는 zinc-binding RING finger motif를 갖고있는 식물 특이적 전사인자로서 식물 생장과 발달에서 중요한 역할을 하고 있다. 배추 생장점 부위로부터 분리된 Brassica rapa SHI-RELATED SEQUENCE (BrSRS) 유전자인 BrSRS7과 BrLRP1은 식물 생장과 발달에서 중요한 조절자이다. BrSRS7과 BrLRP1 유전자가 원예작물의 생장과 발달에 미치는 영향을 보고자 35S 프로모터에 유전자를 결합시켜 두 종류의 식물 형질전환 벡터를 제작한 후 아그로박테리움을 이용하여 페튜니아에 형질전환하였다. 형질전환체들은 전체적으로 식물체 크기가 감소되었고 잎은 작으면서 upward-curled된 특성을 나타내었고 줄기 사이의 길이가 줄었다. 페튜니아의 꽃 모양은 도입된 유전자에 따라 오각형, 별 모양, 둥근 모양으로 다르게 나타났다. 이러한 특성들은 $T_2$, $T_3$ 세대진전 후에도 안정적으로 나타났다. RT-PCR로 유전자 발현을 분석한 결과 BrSRS7과 BrLRP1 유전자는 정단 분열 조직에서 형태 형성에 관여하는 지베릴린과 옥신 관련 유전자인 PtAGL15, PtIAMT1 발현을 조절함으로서 페튜니아의 생장과 발달에 중요한 역할을 하는 것으로 추측된다. 따라서 본 연구결과로 BrSRS 계열 유전자는 왜성 특성, 식물 형태 변형을 갖는 화훼 품종을 개발하는데 유용하게 이용될 수 있을 것으로 사료된다.

SHI-RELATED SEQUENCE (SRS) genes are plant-specific transcription factors that contain a zinc-binding RING finger motif, which play a critical role in plant growth and development. Among Brassica rapa SRS genes, BrSRS7 and BrLRP1 genes, isolated from shoot apical regions are important regulators of plant growth and development. In order to explore the function of BrSRS genes in horticultural plant growth and development, two constructs containing BrSRS7 and BrLRP1 under the control of a cauliflower mosaic virus 35S promoter were introduced into petunia by Agrobacterium-mediated transformation. The resulting transgenic plants were dwarf and compact plants with reduced plant height and diameter. Additionally, these transgenic plants had upward-curled leaves of narrow width and short internodes. Interestingly, the flower shapes of petunia were different among transgenic plants harboring different kinds of SRS genes. These phenotypes were stably inherited through generations $T_2$ and $T_3$. Semi-quantitative RT-PCR analyses of transgenic plants revealed that BrSRS7 and BrLRP1 regulate expression of gibberellin (GA)- and auxinrelated genes, PtAGL15- and PtIAMT1-related, involved in shoot morphogenesis. These results indicate that the overexpression of BrSRS7 and BrLRP1 genes suppressed the growth and development of petunia by regulating expression of GA- and auxin-related genes. From these data, we deduce that BrSRS7 and BrLRP1 genes play an important role in the regulation of plant growth and development in petunia. These findings suggest that transformation with the BrSRS genes can be applied to other species as a tool for growth retardation and modification of plant forms.

키워드

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