• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.6
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• pp.1769-1776
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• 2004

The aim of this study was to find out the effects of the Yukmijihwangtang on transcription activity of Genes related to Bone Morphogenesis. For this purpose, experiments were performed to compare the polyphosphate contents of Yukmijihwangtang and its component herbs, and to verify their Effects on transcription activity of Genes related to Bone Morphogenesis. We know that Yukmijihwangtang and its component herbs have adequate amount of polyphosphate contents and have effects on transcription activity of Genes such as BMP1A, BMP2B, OTN, MGP, COL. In the conclusion, Yukmijihwangtang and its component herbs are strongly believed to have effectiveness on bone morphogenesis.

• Journal of Microbiology
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• v.45 no.1
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• pp.34-40
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• 2007

In budding yeast, G2/M transition is tightly correlated with bud morphogenesis regulated by Swel and septin that plays as a scaffold to recruits protein components. BNI5 isolated as a suppressor for septin defect is implicated in septin organization and cytokinesis. The mechanism by which Bni5 regulates normal septin function is not completely understood. Here, we show that Bni5 phosphorylation is required for mitotic entry regulated by Swel pathway. Bni5 modification was evident from late mitosis to G1 phase, and CIP treatment in vitro of affinity-purified Bni5 removed the modification, indicative of phosphorylation on Bni5. The phosphorylation-deficient mutant of BNI5 (bni5-4A) was defective in both growth at semi-restrictive temperature and suppression of septin defect. Loss of Bni5 phosphorylation resulted in abnormal bud morphology and cell cycle delay at G2 phase, as evidenced by the formation of elongated cells with multinuclei. However, deletion of Swel completely eliminated the elongated-bud phenotypes of both bni5 deletion and bni5-4A mutants. These results suggest that the bud morphogenesis and mitotic entry are positively regulated by phosphorylation-dependent function of Bni5 which is under the control of Swel morphogenesis pathway.

• Journal of Korean Association for Spatial Structures
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• v.6 no.2 s.20
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• pp.107-114
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• 2006

The purpose of this study is to explore the morphology of polyhedra as a tool diversifying the approach to structures and shapes, and to consider the way of the morphogenesis as structural design sources. First, through many examples in nature and styles used as the rhetorical representation of buildings, the shapes of which are based on one of polyhedra are shown. Secondly, the morphogenetic characteristics of polyhedra limited to pure lattice structure are investigated. Thereby the methodology of spatial morphogenesis of polyhedra as structural design sources are generated.

• Korean Institute of Interior Design Journal
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• v.15 no.1 s.54
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• pp.30-38
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• 2006

The new aspect of contemporary architectural design is the computer simulation of morphogenesis and evolution of the organic body. Morphogenesis and evolution is the kind of emergence that is the process of complex pattern formation from simpler rules in complex system. The development comprises the sequence of pattern formation, differentiation, morphogenesis, growth. This study analyzes the application methodology of various biomorphism in contemporary architecture. The methods of generative application by computation in architecture are self-organization, differentiation, growth algorithm via MoSS. And the methods of evolution by computation are genetic algorithm, multi-parameter in environments, phylogenetic cross-over, competing as natural selection, mutation+external constraints, generative algorithm+genetic algorithm via Genr8.

• Journal of Korean Association for Spatial Structures
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• v.10 no.4
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• pp.11-15
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• 2010

• Molecules and Cells
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• v.38 no.6
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• pp.580-586
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• 2015

While increasing evidence indicates the important function of histone methylation during development, how this process influences cardiac development in vertebrates has not been explored. Here, we elucidate the functions of two histone H3 lysine 4 (H3K4) methylation enzymes, SMYD3 and SETD7, during zebrafish heart morphogenesis using gene expression profiling by whole mount in situ hybridization and antisense morpholino oligonucleotide (MO)-based gene knockdown. We find both smyd3 and setd7 are highly expressed within developing zebrafish heart and knock-down of these genes led to severe defects in cardiac morphogenesis without altering the expressions pattern of heart markers, including cmlc2, vmhc, and amhc. Furthermore, double knock-down by coinjection of smyd3 and setd7 MOs caused the synergistic defects in heart development. As similar to knock-down effect, overexpression of these genes also caused the heart morphogenesis defect in zebrafish. These results indicate that histone modifying enzymes, SMYD3 and SETD7, appear to function synergistically during heart development and their proper functioning is essential for normal heart morphogenesis during development.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.6
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• pp.1635-1642
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• 2004

The aim of this study was to find out the effectiveness on germinated Rhynchosia Volubilis for Female Bone Morphogenesis. For this purpose, experiments using germinated Rhynchosia Volubilis(GRV) according to germinating days were conducted to measure the polyphosphate contents and to examine the effects of the transcription activity of gene related to bone morphogenesis on the formation of bone in female. The quantitative analysis of the polyphosphate contents showed that 1 day geminated Rhynchosia Volubilis(GRV) group is treble better contents of polyphosphate than non-germinated Rhynchosia Volubilis, 2 day and 3 day GRV groups. The active of the COL1, OTN, MGP, BMP genes was less than the increase of the polyphosphate contents.

• Animal Systematics, Evolution and Diversity
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• v.21 no.1
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• pp.21-30
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• 2005

The morphogenesis of the marine ciliate, Pelagostrobilidium simile Song and Bradbury, 1998, was investigated using pyridine silver carbonate impregnation. The morphogenesis of P. simile is of hypoapokinetal mode. The oral primordium of P. simile commences slightly below the external membranelles (EM) with the proliferation of an anarchic field. Somatic ciliature in proter and opisthe of P. simile are derived from the old structure with the proliferation of the basal bodies during the dividing process. Parental oral apparatus of P. simile is inherited by the proter, and no reorganization of oral apparatus was observed in the parental oral infraciliature.

• Applied Microscopy
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• v.28 no.3
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• pp.283-297
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• 1998

Hemopoiesis and morphogenesis of the human fetal liver through from 10 to 32 weeks of gestation were investigated by light and electron microscopy. The results obtained were as follows. Hemopoiesis of fetal liver tissue was found from 10 to 32 weeks of gestation, but the hemopoiesis was decreased at 32 weeks of gestation. At the 32 weeks of gestation, matured erythrocytes were observed in the sinusoid, and formation of liver cell cord and portal triad were established. Differentiation of hepatic cell was characterized by the increase of amount of cell organelles within cytoplasm, decrease of hemopoietic cell, morphological change of nuclear envelope from folding form to round form during the developmental period. These results suggest that human fetal liver plays a hematopoietic function until bone marrow and spleen play their function, but morphology of liver at 32 weeks of gestation was differed with structure observed in liver of adult.

• BMB Reports
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• v.53 no.7
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• pp.367-372
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• 2020

Cell cycle-related kinase (CCRK) has a conserved role in ciliogenesis, and Ccrk defects in mice lead to developmental defects, including exencephaly, preaxial polydactyly, skeletal abnormalities, retinal degeneration, and polycystic kidney. Here, we found that Ccrk is highly expressed in mouse trachea and bronchioles. Ccrk mutants exhibited pulmonary hypoplasia and abnormal branching morphogenesis in respiratory organ development. Furthermore, we demonstrated that Ccrk mutant lungs exhibit not only impaired branching morphogenesis but also a significant sacculation deficiency in alveoli associated with reduced epithelial progenitor cell proliferation. In pseudoglandular stages, Ccrk mutant lungs showed a downregulation of Hedgehog (Hh) signaling and defects in cilia morphology and frequency during progenitor-cell proliferation. Interestingly, we observed that activation of the Hh signaling pathway by small-molecule smoothened agonist (SAG) partially rescued bud morphology during branch bifurcation in explants from Ccrk mutant lungs. Therefore, CCRK properly regulates respiratory airway architecture in part through Hh-signal transduction and ciliogenesis.