Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Therapeutic Effects of S-Petasin on Disease Models of Asthma and Peritonitis

  • Lee, Kyoung-Pil (Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University) ;
  • Kang, Saeromi (Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University) ;
  • Noh, Min-Soo (Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University) ;
  • Park, Soo-Jin (Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University) ;
  • Kim, Jung-Min (Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University) ;
  • Chung, Hae Young (Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University) ;
  • Je, Nam Kyung (Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University) ;
  • Lee, Young-Geun (Department of Horticultural Bioscience, College of Natural Resources & Life Science, Pusan National University) ;
  • Choi, Young-Whan (Department of Horticultural Bioscience, College of Natural Resources & Life Science, Pusan National University) ;
  • Im, Dong-Soon (Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University)
  • Received : 2014.06.09
  • Accepted : 2014.07.02
  • Published : 2015.01.01
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Abstract

To explore the anti-allergic and anti-inflammatory effects of extracts of Petasites genus, we studied the effects of s-petasin, a major sesquiterpene from Petasites formosanus (a butterbur species) on asthma and peritonitis models. In an ovalbumin-induced mouse asthma model, s-petasin significantly inhibited the accumulations of eosinophils, macrophages, and lymphocytes in bronchoalveolar fluids. S-petasin inhibited the antigen-induced degranulation of ${\beta}$-hexosamidase but did not inhibit intracellular $Ca^{2+}$ increase in RBL-2H3 mast cells. S-petasin inhibited the LPS induction of iNOS at the RNA and protein levels in mouse peritoneal macrophages. Furthermore, s-petasin inhibited the production of NO (the product of iNOS) in a concentration-dependent manner in the macrophages. Furthermore, in an LPS-induced mouse model of peritonitis, s-petasin significantly inhibited the accumulation of polymorpho nuclear and mononuclear leukocytes in peritoneal cavity. This study shows that s-petasin in Petasites genus has therapeutic effects on allergic and inflammatory diseases, such as, asthma and peritonitis through degranulation inhibition in mast cells, suppression of iNOS induction and production of NO in macrophages, and suppression of inflammatory cell accumulation.

Keywords

References

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