Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Oxidative damage biomarker levels according to Mn-SOD and GST gene polymorphisms in preschool children

Mn-SOD와 GST 유전자 다형성에 따른 유아의 산화손상지표의 분포

  • Shin, You-kyung (Department of Food and Nutrition, Kyung Hee University) ;
  • Choi, Ji-Won (Department of Food and Nutrition, Kyung Hee University) ;
  • Oh, Se-Young (Department of Food and Nutrition, Kyung Hee University) ;
  • Chung, Jayong (Department of Food and Nutrition, Kyung Hee University)
  • 신유경 (경희대학교 생활과학대학 식품영양학과) ;
  • 최지원 (경희대학교 생활과학대학 식품영양학과) ;
  • 오세영 (경희대학교 생활과학대학 식품영양학과) ;
  • 정자용 (경희대학교 생활과학대학 식품영양학과)
  • Received : 2015.09.09
  • Accepted : 2015.10.16
  • Published : 2015.12.31
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Abstract

Purpose: Genetic polymorphisms in antioxidant defense and detoxification genes may modulate the levels of oxidative stress biomarkers. Methods: A total of 301 healthy preschool-aged children in the Seoul and Kyung-gi areas were recruited. DNA was extracted from blood for genotyping of manganese superoxide dismutase (Mn-SOD) Val16Ala, glutathione S-transferase (GST) P1 Ile105Val, GSTT1 present/null, and GSTM1 present/null polymorphisms by PCR-restriction fragment length polymorphism or multiplex PCR analyses. In addition to a questionnaire survey, the levels of urinary 8-hydroxyl-2-deoxiguanosine (8-OHdG) and plasma malondialdehyde (MDA) were measured by ELISA. Results: Significantly higher urinary 8-OHdG concentrations were observed in GSTP1 Ile/Val + Val/Val genotype (p = 0.030), and tended to be higher in Mn-SOD Val/Val genotype (p = 0.065). On the other hand, exposure to environmental tobacco smoking (ETS) and interaction between ETS and gene polymorphisms did not significantly influence either urinary 8-OHdG concentrations or serum MDA. Conclusion: Based on our findings, GSTP1 Ile/Val gene polymorphisms might modulate the levels of oxidative stress biomarkers in healthy preschool children.

우리나라 일부 건강한 유아를 대상으로 Mn-SOD Val16Ala, GSTP1 Ile105Val, GSTT1 present/null, GSTM1 present/null 유전자 다형성 분포를 살펴본 결과, Mn-SOD Val/Val형, GSTP1 Ile/Ile형, GSTT1 null 형, GSTM1 null 형이 주된 (major) 유전자형인 것으로 나타났다. 이 중 Mn-SOD Val/Val형은 Val/Ala 또는 Ala/Ala형에 비해 소변 8-OHdG 수준이 유의적이지는 않으나 높은 경향을 나타내었고, GSTP1 Ile/Ile형은 Ile/Val 또는 Val/Val형에 비해 소변 8-OHdG 수준이 유의적으로 낮았다. 간접흡연에의 노출 여부와 간접흡연-유전자 다형성의 상호 작용이 산화손상지표에는 유의적인 영향을 미치지 않는 것으로 나타났다. 이상의 결과로 볼 때 건강한 유아에서 GSTP1 Val allele 보유한 경우 산화적 손상에 대해 취약할 수 있음을 제시하며, 추후 대규모 연구를 통한 검증 및 이들 유전자형을 보유한 대상자를 위한 효과적인 영양 중재방안에 대한 고려가 필요할 것으로 사료된다.

Keywords

References

  1. Ames BN. Dietary carcinogens and anticarcinogens. Oxygen radicals and degenerative diseases. Science 1983; 221(4617): 1256-1264. https://doi.org/10.1126/science.6351251
  2. Zhang H, Davies KJ, Forman HJ. Oxidative stress response and Nrf2 signaling in aging. Free Radic Biol Med 2015; 8(Pt B): 314-336.
  3. Thanan R, Oikawa S, Hiraku Y, Ohnishi S, Ma N, Pinlaor S, Yongvanit P, Kawanishi S, Murata M. Oxidative stress and its significant roles in neurodegenerative diseases and cancer. Int J Mol Sci 2015; 16(1): 193-217. https://doi.org/10.3390/ijms16010193
  4. Dikalov SI, Ungvari Z. Role of mitochondrial oxidative stress in hypertension. Am J Physiol Heart Circ Physiol 2013; 305(10): H1417-H1427. https://doi.org/10.1152/ajpheart.00089.2013
  5. Yan MH, Wang X, Zhu X. Mitochondrial defects and oxidative stress in Alzheimer disease and Parkinson disease. Free Radic Biol Med 2013; 62: 90-101. https://doi.org/10.1016/j.freeradbiomed.2012.11.014
  6. Zhu H, Li YR. Oxidative stress and redox signaling mechanisms of inflammatory bowel disease: updated experimental and clinical evidence. Exp Biol Med (Maywood) 2012; 237(5): 474-480. https://doi.org/10.1258/ebm.2011.011358
  7. Kim YJ, Kim EH, Hahm KB. Oxidative stress in inflammationbased gastrointestinal tract diseases: challenges and opportunities. J Gastroenterol Hepatol 2012; 27(6): 1004-1010. https://doi.org/10.1111/j.1440-1746.2012.07108.x
  8. Shimoda-Matsubayashi S, Matsumine H, Kobayashi T, Nakagawa- Hattori Y, Shimizu Y, Mizuno Y. Structural dimorphism in the mitochondrial targeting sequence in the human manganese superoxide dismutase gene. A predictive evidence for conformational change to influence mitochondrial transport and a study of allelic association in Parkinson's disease. Biochem Biophys Res Commun 1996; 226(2): 561-565. https://doi.org/10.1006/bbrc.1996.1394
  9. Klaunig JE, Kamendulis LM, Hocevar BA. Oxidative stress and oxidative damage in carcinogenesis. Toxicol Pathol 2010; 38(1): 96-109. https://doi.org/10.1177/0192623309356453
  10. Bresciani G, Cruz IB, de Paz JA, Cuevas MJ, Gonzalez-Gallego J. The MnSOD Ala16Val SNP: relevance to human diseases and interaction with environmental factors. Free Radic Res 2013; 47(10): 781-792. https://doi.org/10.3109/10715762.2013.836275
  11. Duarte MM, Moresco RN, Duarte T, Santi A, Bagatini MD, Da Cruz IB, Schetinger MR, Loro VL. Oxidative stress in hypercholesterolemia and its association with Ala16Val superoxide dismutase gene polymorphism. Clin Biochem 2010; 43(13-14): 1118-1123. https://doi.org/10.1016/j.clinbiochem.2010.07.002
  12. Knapen MF, Zusterzeel PL, Peters WH, Steegers EA. Glutathione and glutathione-related enzymes in reproduction. A review. Eur J Obstet Gynecol Reprod Biol 1999; 82(2): 171-184. https://doi.org/10.1016/S0301-2115(98)00242-5
  13. Celik SK, Aras N, Yildirim O, Turan F, Gorur A, Yildirim H, Tamer L. Glutathione S-transferase GSTM 1, null genotype may be associated with susceptibility to age-related cataract. Adv Clin Exp Med 2015; 24(1): 113-119. https://doi.org/10.17219/acem/38143
  14. Goodrich JM, Basu N. Variants of glutathione s-transferase pi 1 exhibit differential enzymatic activity and inhibition by heavy metals. Toxicol In Vitro 2012; 26(4): 630-635. https://doi.org/10.1016/j.tiv.2012.02.005
  15. Suvakov S, Damjanovic T, Stefanovic A, Pekmezovic T, Savic- Radojevic A, Pljesa-Ercegovac M, Matic M, Djukic T, Coric V, Jakovljevic J, Ivanisevic J, Pljesa S, Jelic-Ivanovic Z, Mimic-Oka J, Dimkovic N, Simic T. Glutathione S-transferase A1, M1, P1 and T1 null or low-activity genotypes are associated with enhanced oxidative damage among haemodialysis patients. Nephrol Dial Transplant 2013; 28(1): 202-212. https://doi.org/10.1093/ndt/gfs369
  16. Brunst KJ, Baccarelli AA, Wright RJ. Integrating mitochondriomics in children's environmental health. J Appl Toxicol 2015; 35(9): 976-991. https://doi.org/10.1002/jat.3182
  17. Ahn K. The role of air pollutants in atopic dermatitis. J Allergy Clin Immunol 2014; 134(5): 993-999 . https://doi.org/10.1016/j.jaci.2014.09.023
  18. McCrindle BW. Cardiovascular consequences of childhood obesity. Can J Cardiol 2015; 31(2): 124-130. https://doi.org/10.1016/j.cjca.2014.08.017
  19. Akyol O, Canatan H, Yilmaz HR, Yuce H, Ozyurt H, Sogut S, Gulec M, Elyas H. PCR/RFLP-based cost-effective identification of SOD2 signal (leader) sequence polymorphism (Ala-9Val) using NgoM IV: a detailed methodological approach. Clin Chim Acta 2004; 345(1-2): 151-159. https://doi.org/10.1016/j.cccn.2004.03.021
  20. Wilson MH, Grant PJ, Hardie LJ, Wild CP. Glutathione S-transferase M1 null genotype is associated with a decreased risk of myocardial infarction. FASEB J 2000; 14(5): 791-796. https://doi.org/10.1096/fasebj.14.5.791
  21. Sutton A, Khoury H, Prip-Buus C, Cepanec C, Pessayre D, Degoul F. The Ala16Val genetic dimorphism modulates the import of human manganese superoxide dismutase into rat liver mitochondria. Pharmacogenetics 2003; 13(3): 145-157. https://doi.org/10.1097/00008571-200303000-00004
  22. Becer E, Cirakoglu A. Association of the Ala16Val MnSOD gene polymorphism with plasma leptin levels and oxidative stress biomarkers in obese patients. Gene 2015; 568(1): 35-39. https://doi.org/10.1016/j.gene.2015.05.009
  23. Zimniak P, Nanduri B, Pikula S, Bandorowicz-Pikula J, Singhal SS, Srivastava SK, Awasthi S, Awasthi YC. Naturally occurring human glutathione S-transferase GSTP1-1 isoforms with isoleucine and valine in position 104 differ in enzymic properties. Eur J Biochem 1994; 224(3): 893-899. https://doi.org/10.1111/j.1432-1033.1994.00893.x
  24. Watson MA, Stewart RK, Smith GB, Massey TE, Bell DA. Human glutathione S-transferase P1 polymorphisms: relationship to lung tissue enzyme activity and population frequency distribution. Carcinogenesis 1998; 19(2): 275-280. https://doi.org/10.1093/carcin/19.2.275
  25. Aynacioglu AS, Nacak M, Filiz A, Ekinci E, Roots I. Protective role of glutathione S-transferase P1 (GSTP1) Val105Val genotype in patients with bronchial asthma. Br J Clin Pharmacol 2004; 57(2): 213-217. https://doi.org/10.1046/j.1365-2125.2003.01975.x
  26. Tamer L, Calikoglu M, Ates NA, Yildirim H, Ercan B, Saritas E, Unlu A, Atik U. Glutathione-S-transferase gene polymorphisms (GSTT1, GSTM1, GSTP1) as increased risk factors for asthma. Respirology 2004; 9(4): 493-498. https://doi.org/10.1111/j.1440-1843.2004.00657.x
  27. Lee E, Chang HY, Lee KS, Suh DI, Yu HS, Kang MJ, Choi IA, Park J, Kim KW, Shin YH, Ahn KM, Kwon JY, Choi SJ, Lee KJ, Won HS, Yang SI, Jung YH, Kim HY, Seo JH, Kwon JW, Kim BJ, Kim HB, Lee SY, Kim EJ, Lee JS, Keyes KM, Shin YJ, Hong SJ; COCOA study group. The effect of perinatal anxiety on bronchiolitis is influenced by polymorphisms in ROS-related genes. BMC Pulm Med 2014; 14: 154. https://doi.org/10.1186/1471-2466-14-154
  28. Reddy P, Naidoo RN, Robins TG, Mentz G, London SJ, Li H, Naidoo R. GSTM1, GSTP1, and NQO1 polymorphisms and susceptibility to atopy and airway hyperresponsiveness among South African schoolchildren. Lung 2010; 188(5): 409-414. https://doi.org/10.1007/s00408-010-9246-3
  29. Karam RA, Pasha HF, El-Shal AS, Rahman HM, Gad DM. Impact of glutathione-S-transferase gene polymorphisms on enzyme activity, lung function and bronchial asthma susceptibility in Egyptian children. Gene 2012; 497(2): 314-319. https://doi.org/10.1016/j.gene.2012.01.059
  30. Jo HR, Lee HJ, Kang MH. Antioxidative status, DNA damage and lipid profiles in Korean young adults by glutathione S-transferase polymorphisms. Korean J Nutr 2011; 44(1): 16-28. https://doi.org/10.4163/kjn.2011.44.1.16
  31. Gergen PJ, Fowler JA, Maurer KR, Davis WW, Overpeck MD. The burden of environmental tobacco smoke exposure on the respiratory health of children 2 months through 5 years of age in the United States: Third National Health and Nutrition Examination Survey, 1988 to 1994. Pediatrics 1998; 101(2): E8.
  32. Yi O, Kwon HJ, Kim H, Ha M, Hong SJ, Hong YC, Leem JH, Sakong J, Lee CG, Kim SY, Kang D. Effect of environmental tobacco smoke on atopic dermatitis among children in Korea. Environ Res 2012; 113: 40-45. https://doi.org/10.1016/j.envres.2011.12.012
  33. Ministry of Health and Welfare, Korea Centers for Disease Control and Prevention. Korean adults male smoking rate [Internet]. Cheongju: Ministry of Health and Welfare; 2015 [cited 2015 Sep 8]. Available from : http://kosis.kr.
  34. Holloway JW, Savarimuthu Francis S, Fong KM, Yang IA. Genomics and the respiratory effects of air pollution exposure. Respirology 2012; 17(4): 590-600. https://doi.org/10.1111/j.1440-1843.2012.02164.x
  35. Wu J, Hankinson J, Kopec-Harding K, Custovic A, Simpson A. Interaction between glutathione S-transferase variants, maternal smoking and childhood wheezing changes with age. Pediatr Allergy Immunol 2013; 24(5): 501-508. https://doi.org/10.1111/pai.12086
  36. Chiou CC, Chang PY, Chan EC, Wu TL, Tsao KC, Wu JT. Urinary 8-hydroxydeoxyguanosine and its analogs as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers. Clin Chim Acta 2003; 334(1-2): 87-94. https://doi.org/10.1016/S0009-8981(03)00191-8
  37. Forlenza MJ, Miller GE. Increased serum levels of 8-hydroxy-2'- deoxyguanosine in clinical depression. Psychosom Med 2006; 68(1): 1-7.
  38. Carvalho AM, Miranda AM, Santos FA, Loureiro AP, Fisberg RM, Marchioni DM. High intake of heterocyclic amines from meat is associated with oxidative stress. Br J Nutr 2015; 113(8): 1301-1307. https://doi.org/10.1017/S0007114515000628
  39. Il'yasova D, Scarbrough P, Spasojevic I. Urinary biomarkers of oxidative status. Clin Chim Acta 2012; 413(19-20): 1446-1453. https://doi.org/10.1016/j.cca.2012.06.012
  40. Soto-Mendez MJ, Aguilera CM, Campana-Martin L, Martin- Laguna V, Schumann K, Solomons NW, Gil A. Variation in hydration status within the normative range is associated with urinary biomarkers of systemic oxidative stress in Guatemalan preschool children. Am J Clin Nutr 2015; 102(4): 865-872. https://doi.org/10.3945/ajcn.114.105429
  41. Oztop D, Altun H, Baskol G, Ozsoy S. Oxidative stress in children with attention deficit hyperactivity disorder. Clin Biochem 2012; 45(10-11): 745-748. https://doi.org/10.1016/j.clinbiochem.2012.03.027
  42. Al-Alem U, Gann PH, Dahl J, van Breemen RB, Mistry V, Lam PM, Evans MD, Van Horn L, Wright ME. Associations between functional polymorphisms in antioxidant defense genes and urinary oxidative stress biomarkers in healthy, premenopausal women. Genes Nutr 2012; 7(2): 191-195. https://doi.org/10.1007/s12263-011-0257-3
  43. Prasad SB, Vidyullatha P, Vani GT, Devi RP, Rani UP, Reddy PP, Prasad HM. Association of gene polymorphism in detoxification enzymes and urinary 8-OHdG levels in traffic policemen exposed to vehicular exhaust. Inhal Toxicol 2013; 25(1): 1-8. https://doi.org/10.3109/08958378.2012.745634
  44. He FF, Li HQ, Huang QX, Wang QY, Jiang HJ, Chen S, Su H, Zhang C, Wang YM. Tumor Necrosis Factor-Alpha and 8- Hydroxy-2'-Deoxyguanosine are Associated with Elevated Urinary Angiopoietin-2 Level in Type 2 Diabetic Patients with Albuminuria. Kidney Blood Press Res 2015; 40(4): 355-365. https://doi.org/10.1159/000368510
  45. Omata N, Tsukahara H, Ito S, Ohshima Y, Yasutomi M, Yamada A, Jiang M, Hiraoka M, Nambu M, Deguchi Y, Mayumi M. Increased oxidative stress in childhood atopic dermatitis. Life Sci 2001; 69(2): 223-228. https://doi.org/10.1016/S0024-3205(01)01124-9