Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지
DOI QR코드

DOI QR Code

Growth Inhibition of In Vitro Plantlets and Improvement of Survival Rate of Acclimated Plant of Strawberry according to Polyethylene Glycol during Bioreactor Culture

딸기 조직배양묘의 생물반응기 배양 시 Polyethylene Glycol 처리에 따른 기내 생육억제 효과 및 순화율 향상

  • Kim, Hye Jin (Highland Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Lee, Jong Nam (Highland Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Kim, Ki Deog (Highland Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Lim, Hak Tae (Department of Bio-Health Technology, Kangwon National University) ;
  • Yeoung, Young Rok (Department of Plant Science, Gangneung-Wonju National University)
  • 김혜진 (국립식량과학원 고령지농업연구소) ;
  • 이종남 (국립식량과학원 고령지농업연구소) ;
  • 김기덕 (국립식량과학원 고령지농업연구소) ;
  • 임학태 (강원대학교 생명건강공학전공) ;
  • 용영록 (강릉원주대학교 식물생명과학과)
  • Received : 2015.02.13
  • Accepted : 2015.06.22
  • Published : 2015.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out treatment of polyethylene glycol (PEG) in order to increase of survival rate of acclimated plants of strawberry's in vitro plantlets through bioreactor culture. We used PEG with molecular weight 6000 (PEG 6000) in this study. Concentration of PEG is non-treatment, 5, 10, 15, and $20g{\cdot}L^{-1}$ each bioreactor. $5g{\cdot}L^{-1}$ of PEG was treated at $1^{st}$, $2^{nd}$, $3^{rd}$, $4^{th}$, and $5^{th}$ week during culture. We investigated growth characteristics of in vitro plantlets after 6 weeks cultivation. Growth amount of all PEG treatment decreased as compared to non-treatment. The more concentration increased, the more plant growth decreased. In $5g{\cdot}L^{-1}$ of PEG, shoot length was shorter than non-treatment that shoot length was 7.9 cm and especially fresh weight that is 1.6 g was more decrease than non-treatment. Shoot length was ranged 3.0-3.9 cm at $1^{st}$ week treatment to $4^{th}$ week treatment of $5g{\cdot}L^{-1}$ PEG. The shoot length was not significant by 5.3 cm at $5^{th}$ week treatment. The survival rate was improved 5.4% at the treatment of $4^{th}$ week and was improved 8.7% at the $5^{th}$ week as compared to non-treatment. In order to improve of survival rate of strawberry' in vitro plantlets through bioreactor culture, the method is suitable that adding $5g{\cdot}L^{-1}$ of PEG in the medium and $5^{th}$ week's treatment is suitable.

본 실험은 생물반응기를 이용한 딸기 조직배양묘의 대량증식 시 순화 생존율 향상을 위하여 polyethylene glycol(PEG 6000)을 농도별, 처리시기별로 실험하였고 기내생육조사는 배양 6주 후에 실시하였다. 모든 PEG처리구의 생장량은 대조구에 비해 매우 감소하였고, 농도가 높아질수록 생장억제 효과가 급격히 나타났다. PEG $5g{\cdot}L^{-1}$처리 시 초장은 대조구의 7.9cm보다 매우 짧아졌고, 특히 생체중은 1.60g으로 대조구보다 감소하였다. PEG $5g{\cdot}L^{-1}$의 배양1주차에서 4주차까지의 처리시 초장이 3.0-3.9cm 범위로 대조구보다 매우 짧았으나, 배양5주차 처리의 초장은 5.3cm로 큰 차이를 보이지 않았다. PEG $5g{\cdot}L^{-1}$의 처리시기별 순화 생존율은 대조구에 비해 배양4주 처리에 5.4%, 배양5주차 처리에 8.7% 향상되었다. 따라서 생물반응기를 통해 대량증식된 딸기 조직배양묘의 순화율 향상을 위해 PEG는 $5g{\cdot}L^{-1}$의 농도로 배양 5주차에 처리하는 것이 적당하였다.

Keywords

References

  1. Blum, A. 2013. Use of PEG to induce and control plant water deficit in experimental dydroponics culture. www.plantstress.com/method/peg.html.
  2. Burnett, S.E. 2004. Effects of polyethylene glysol on the morphology of ornamental seedlings. PhD diss. Univ. Ga., Athens.
  3. Burnett, S.E., P. Thomas, and M. van Iersel. 2005. Postgermination drenches with PEG-8000 reduce growth of salvia and marigolds. HortScience 40:675-679.
  4. Dhawan, V. and S.S. Bhojwani. 1987. Hardening in vitro and morpho-physiological changes in the leaves during acclimatization of micropropagated plants of Leucaena leucocephala (LAM.) de wit. Plant Sci. 53:65-72. https://doi.org/10.1016/0168-9452(87)90179-8
  5. Hamayun, M., S.A. Khan, Z.K. Shinwari, A.L. Khan, N. Ahmed, and I.J. Lee. 2010. Effect of polyethylene glycol induced drought stress on physio-hormonal attributes of soybean. Pak. J. Bot. 42:977-986.
  6. Jordan, W.R., P.J. Shouse, R.L., A. Blum, F.R. Miller, and R.L. Monk. 1984. Environmental physiology of Sorghum. II. Epicuticular wax load and cuticular transpiration. Crop Sci. 24:1168-1173. https://doi.org/10.2135/cropsci1984.0011183X002400060038x
  7. Kim, H.J., J.N. Lee, K.D. Kim, J.S. Im, H.T. Lim, and Y.R. Yeoung. 2011. Suitable hormone-free medium for in vitro mass propagation via bioreactor culture of ever-bearing strawberry. J. Plant Biotechnol. 38:221-227. https://doi.org/10.5010/JPB.2011.38.3.221
  8. Kim, H.J., J.N. Lee, K.D. Kim, J.S. Im, H.T. Lim, and Y.R. Yeoung. 2012. Growth characteristics of in vitro mass propagated plantlets of ever-bearing strawberry 'Goha' according to aeration rate in bioreactor. Korean J. Hortic. Sci. Technol. 30:432-436. https://doi.org/10.7235/hort.2012.12053
  9. Largerwerff, J.V., G. Ogata, and H.E. Eagle. 1961. Control of osmotic pressure of culture solutions with polyethylene glycol. Science 133:1486. https://doi.org/10.1126/science.133.3463.1486
  10. Lee, J.N., H.J. Kim, K.D. Kim, Y.S. Kwon, J.S. Im, H.T. Lim, and Y.R. Yeoung. 2010a. In vitro mass propagation and economic effects of bioreactor culture in ever-bearing strawberry 'Goha'. Korean J. Hortic. Sci. Technol. 28:845-849.
  11. Lee, J.N., H.J. Kim, K.D. Kim, Y.S. Kwon, Y.R. Yeoung, and H.T. Lim. 2010b. Appropriate in vitro culture conditions of growing medium for new ever-bearing strawberry 'Goha'. Korean J. Hortic. Sci. Technol. 28:1051-1056.
  12. Lee, J.N., H.J. Kim, K.D. Kim, J.S. Im, H.T. Lim, and Y.R. Yeoung. 2012. Growth characteristics and economic efficiency of nursery plants production according to transplanting container for acclimatization of mass propagated plantlets via bioreactor culture of ever-bearing strawberry 'Goha'. Korean J. Hortic. Sci. Technol. 30:437-441. https://doi.org/10.7235/hort.2012.12054
  13. Lewandowski, V.T. 1991. Rooting and acclimatization of micropropagated Vitis labrusca 'Delaware'. HortScience 26:586-589.
  14. Macar, T.K., T. Ozlem, and Y. Ekmekci. 2009. Effect of water deficit induced by PEG and NaCl on chickpea (Cicer arientinum L.) cultivars and lines at early seedling stages. Gazi Univ. J. Sci. 22:5-14.
  15. McClelland, M.T. and M.A.L. Smith. 1990. Vessel type, closure, and explants orientation influence in vitro performance of five woody species. HortScience 25:797-800.
  16. Sutter, E.G. 1988. Stomata and cuticular water loss from apple, cherry, and sweet-gum plants after removal from in vitro culture. J. Am. Soc. Hortic. Sci. 113:234-238.
  17. Sutter, E.G. and R.W. Langhans. 1979. Epicuticular wax formation on Carnation plantlets regenerated from shoot-tip culture. J. Am. Soc. Hortic. Sci. 104:493-496.
  18. Xu, C. and B. Huang. 2010. Differential proteomic responses to water stress induced by PEG in two creeping bentgrass cultivars differing in stress tolerance. J. Plant Physiol. 167:1477-1485. https://doi.org/10.1016/j.jplph.2010.05.006
  19. Zaid, A. and H. Hughes. 1995. In vitro acclimatization of date palm (Phoenix dactylifera L.) plantlets : A quantitative comparison of epicuticular leaf wax as a function of polyethylene glycol treatment. Plant Cell Rep. 15:111-114. https://doi.org/10.1007/BF01690265