Reproductive and Developmental Biology

  • 제38권3호
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  • Pages.115-121
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  • 2014
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
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소에서 정자활성, 수정 양상 및 착상전 지속적 수정란 발달에 있어서 환삼덩굴 추출액의 효과

Effect of Humulus japonicus Extract on Sperm Motility, Fertilization Status and Subsequent Preimplantation Embryo Development in Cattle

  • 민성훈 (대구대학교 공과대학 생명공학과) ;
  • 김진우 (대구대학교 공과대학 생명공학과) ;
  • 도건엽 (대구대학교 공과대학 생명공학과) ;
  • 이용희 (대구대학교 공과대학 생명공학과) ;
  • 안재현 (대구대학교 공과대학 생명공학과) ;
  • 채성규 (대구대학교 공과대학 생명공학과) ;
  • 김병오 (경북대학교 농업생명과학대학 식품공학부) ;
  • 박흠대 (대구대학교 공과대학 생명공학과) ;
  • 구덕본 (대구대학교 공과대학 생명공학과)
  • Min, Sung-Hun (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Kim, Jin-Woo (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Do, Geon-Yeop (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Lee, Yong-Hee (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Ahn, Jae-Hyun (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Chae, Sung-Kyu (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Kim, Byung Oh (School of Food Science & Biotechnology, College of Agriculture & Life Sciences, Kyungpook National University) ;
  • Park, Humdai (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Koo, Deog-Bon (Department of Biotechnology, College of Engineering, Daegu University)
  • 투고 : 2014.09.05
  • 심사 : 2014.09.17
  • 발행 : 2014.09.30
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초록

Humulus japonicus is an ornamental plant in the Cannabaceae family. Although the mode of action of Humulus japonicus is not fully understood, a strong relationship was observed between anti-inflammatory and anticancer in some types of cells. Recent studies also have shown that Humulus japonicus possesses anti-inflammatory activities and may significantly improve antioxidant potential in Raw 264.7 macrophage cells. Thus, the aim of this study was evaluated the effect of Humulus japonicus extract on sperm motility and subsequent preimplantation developmental competence of the bovine embryos. After in vitro maturation, the oocytes with sperms were exposed in in vitro fertilization (IVF) medium supplemented with Humulus japonicus extract (0.01, 0.05, $0.1{\mu}g/mL$, respectively) for 1 day. In our results, exposure of IVF medium to Humulus japonicus extract did not affect sperm motility and percentage of penetrated oocytes but ROS intensity was significantly decreased by $0.01{\mu}g/mL$ compared with other groups (p< 0.05). Moreover, treatment with $0.01{\mu}g/mL$ of Humulus japonicus extract was higher the frequency of blastocyst formation than the any other groups (p<0.05). Otherwise, treatment with $0.01{\mu}g/mL$ of Humulus japonicus extract not increased the total cell number but reduced apoptotic-positive nuclei number. In conclusion, our results indicate that supplementation of Humulus japonicus extract in IVF medium may have important implications for improving early embryonic development in bovine embryos.

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