• Reproductive and Developmental Biology
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• v.38 no.3
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• pp.115-121
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• 2014

Humulus japonicus is an ornamental plant in the Cannabaceae family. Although the mode of action of Humulus japonicus is not fully understood, a strong relationship was observed between anti-inflammatory and anticancer in some types of cells. Recent studies also have shown that Humulus japonicus possesses anti-inflammatory activities and may significantly improve antioxidant potential in Raw 264.7 macrophage cells. Thus, the aim of this study was evaluated the effect of Humulus japonicus extract on sperm motility and subsequent preimplantation developmental competence of the bovine embryos. After in vitro maturation, the oocytes with sperms were exposed in in vitro fertilization (IVF) medium supplemented with Humulus japonicus extract (0.01, 0.05, $0.1{\mu}g/mL$, respectively) for 1 day. In our results, exposure of IVF medium to Humulus japonicus extract did not affect sperm motility and percentage of penetrated oocytes but ROS intensity was significantly decreased by $0.01{\mu}g/mL$ compared with other groups (p< 0.05). Moreover, treatment with $0.01{\mu}g/mL$ of Humulus japonicus extract was higher the frequency of blastocyst formation than the any other groups (p<0.05). Otherwise, treatment with $0.01{\mu}g/mL$ of Humulus japonicus extract not increased the total cell number but reduced apoptotic-positive nuclei number. In conclusion, our results indicate that supplementation of Humulus japonicus extract in IVF medium may have important implications for improving early embryonic development in bovine embryos.

• The Korean Journal of Food And Nutrition
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• v.25 no.2
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• pp.357-361
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• 2012

To evaluate the antioxidant and potential of Humulus japonicus, total polyphenol and flavonoid content, radical scavenging activities, and antitumor activities were measured. The total polyphenol and flavonoid contents of the methanol extracts from Humulus japonicus were $30.13{\pm}1.13$ and $13.61{\pm}0.49$ mg gallic acid equivalent/g extract, respectively. DPPH radical and hydroxyl radical scavenging activities of methanol extracts of Humulus japonicus were 60% and 35%, respectively. The Humulus japonicus higher activities of anticancer activities on liver cancer cell lines compared to other cancer cell lines.

• Korean Journal of Weed Science
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• v.10 no.2
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• pp.75-82
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• 1990

Weed species investigated along the waterway of Kyungnam and Kyungpook provinces on September, 1989 were 88 species in 33 families and 96 species in 33 families, respectively. Three weed community types were observed along the waterway of Kyungnam province, namely, Echiuochoa cars-galli, Persicaria hydropiper and Persicaria thunbergii, and five community types such as Humulus japonicus-bideus froudosa, Solanum nigrum, Persicaria thunbergii-Humulus japonicus, Humulus japonicus and Persicaria thunbergii were detected along the waterway of Kyungpook province. The most dominant weed species indentified along the waterways in both Kyungnam and Kyungpook provinces were Pensicaria tlrutrheagii and Persicaria hydropiper which are the aquatic weed species.

• Korean Journal of Food Science and Technology
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• v.27 no.6
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• pp.897-901
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• 1995

To investigate the physiological activities of Humulus japonicus, we examined the mutagenic and antimutagenic effects of the extract and isolated the flavonoid compounds. The water and methanol extracts of Humulus japonicus did not show the mutagenicity by Ames test in the 0.5-4 mg/plate. The methanol extract showed the antimutagenic effect aganinst Salmonella typhimurium TA98 and TA100 induced by 4-nitro-o-phenylenediamine(NPD) and 4-nitroquinoline-N-oxide(NQO). The hexane fraction showed comparatively higher antimutagenic effect than other solvent fractions from the methanol extract. The 50% inhibition concentration($IC_{50}$) of hexane fraction were 2.0 mg/plate, 0.5 mg/plate in TA98 and TA100 respectively. Quercitrin and luteolin were identified in the ethyl acetate fraction from methanol extract of Humulus japonicus by TLC and HPLC.

• The Journal of Pediatrics of Korean Medicine
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• v.37 no.3
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• pp.121-132
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• 2023

Objectives We aimed to confirm whether Humulus japonicus Extract Powder can enhance child height growth significantly and safely compared with a placebo. Methods A total of 150 children between the 3rd and 25th percentiles in height and between the ages of 6 and 9 years will be recruited to participate in this randomized, double-blind, placebo-controlled clinical trial. The participants will be randomly assigned to the treatment or placebo group. Participants in the treatment group will take one pack per day (700 mg of Humulus japonicus Extract Powder) for 24 weeks. Participants in the placebo group will take one package of placebo per day (0 mg of Humulus japonicus Extract Powder) for 24 weeks. The primary outcome will be a change in height after 12 weeks, and the secondary outcomes will be the height after 24 weeks, growth rate, height standard deviation, growth hormone, insulin-like growth factor-1 (IGF-1), insulin-like growth factor binding protein-3 (IGFBP-3), bone alkaline phosphatase (BALP), and osteocalcin after 12 and 24 weeks. Results This protocol was approved by the Institutional Review Board (IRB) of the Korean Medicine Hospital of Busan University (IRB No. PNUKHIRB-2023-03-002). Research participants will be recruited from June 2023 to December 2023. Conclusions The results of this study provide clinical information regarding the effectiveness and safety of the Humulus japonicus Extract Powder in increasing child height.

• Textile Coloration and Finishing
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• v.26 no.3
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• pp.263-271
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• 2014

The purpose of this study is to investigate the dyeability of silk fabrics with Humulus japonicus (Hwansam) extract which is a kind of weeds that grows wild in the suburbs. In the research, the general extract method was conducted with the extract from fresh herb of root-removed Humulus japonicus while we investigated the optimal dyeing conditions and dyeability depending on the changes of the kind of mordants, methods and temperature. The functional properties such as antibacterial activities, deodorization and UV protection were also reviewed. The ideal output, as a result, was shown at 100% of dye concentration, $90^{\circ}C$ of dyeing temperature and 40 minutes of dyeing time while in terms of dye uptake depending on the kind of mordants and methods, it was found that among four mordants of $SnCl_2$, $AlK(SO_4)_2$, $CuSO_4$, and $FeSO_4$ post-mordanting with $FeSO_4$ showed the best result. Color fastness to washing, dry cleaning and rubbing was found strong at grade 4-5 and the grade to light was as good as 3 while to perspiration was good at 4-5 as well. In aspects of functional properties, it showed excellent results of 99% deodorization rate, 98% UV protection rate and 99.9% bacterial reduction against Staphylococcus aureus.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.112-112
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• 2022

This study aims to confirm the possibility of using the invasive alien plants in Jeju as a functional biomaterial. To achieve this purpose, 70% ethanol extract and solvent fractions were prepared for five invasive alien plants (Hypochaeris radicata, Rumex acetosella, Humulus japonicus Siebold & Zucc., Solanum viarum, Lactuca scariolar) and their antioxidant, antibacterial anti-inflammatory and anti-obesity effects were investigated. The DPPH radical scavenging activity of ethanol extract from invasive alien plants was shown in the order of Rumex acetosella > Hypochaeris radicata > Humulus japonicus. Antimicrobial activity of ethanol extract against food poisoning bacteria (4 species) and oral cavity-induced microorganisms (6 species) was measured. As a result, the extract of Humulus japonicus showed high antibacterial effects against food poisoning bacteria (E. coli, V. parahaemolyticus) and oral microbes (L. casei, S. epidermidis, E. faecalis). In LPS-induced RAW 264.7 cells, the anti-inflammatory effect of ethanol extract from invasive alien plants was investigated. As a result, the NO production inhibition activity was highest in the Rumex acetosella and the Humulus japonicus Siebold & Zucc. ethanol extract, and the NO production inhibition activity was concentration-dependent. In addition, the Rumex acetosella and the Humulus japonicus Siebold & Zucc. ethanol extract showed a concentration-dependent inhibitory effect on cytokine (IL-6) production. These extracts also showed inhibitory activity of COX-2, an inflammatory protein. This suggests that NO production inhibition activity by the extract of invasive alien plants is the result of inhibition of iNOS and COX-2 expression. Currently, organic solvent fractions of crude extract are manufactured and the investigation of active ingredients is continuing along with evaluation of biological activity such as anti-inflammatory. These results are expected to be a major data for the study on the separation and utilization of active ingredients with antioxidant, antibacterial and anti-inflammatory effects using foreign plant crude extract and solvent fractions, and are highly likely to be applied to the development of functional food and cosmetics materials.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.1
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• pp.106-110
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• 2000

We investigated the active oxygen scavenging activity and active compound from Humulus japonicus. The ethyl acetate and butanol fraction exhibited strong scavenging effects on hydroxyl radical. Furthermore active compound was isolated and purified using Amberlite XAD-2 column chromatography and preparative HPLC from ethyl acetate fracton, and major compound was identified as a luteolin-7-O-$\beta$-D-glucoside by the MS, UV, 1H-NMR and 13C-NMR analyses. Luteolin-7-O-$\beta$-D-glucoside exhibited strong scavenging effect on active oxygens such as superoxide radical, hydroxyl radical and hydrogen peroxide.

• Journal of Ecology and Environment
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• v.29 no.5
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• pp.461-467
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• 2006

The growth pattern and the seasonal changes in nutrient contents of Humulus japonicus were investigated. Stem length of H. japonicus reached the maximum from 240 to 260 Julian date and the median value was found at 255 Julian date. The maximum leaf area was observed from 235 to 248 Julian date and the median value was at 240 Julian date. The maximum growth rate of the stem length ranged from 205 to 227 Julian date. The leaf area showed the maximum growth rate from 196 to 214 Julian date. The median date in the growth rate of the stem length and leaf area was 212 and 205 Julian date, respectively. The growth rate of H. japonicus was related to rainy season and precipitation. Phosphorus and sodium contents of H. japonicus were correlated with maximum potential rate of relative growth. Although stem biomass of H. japonicus was $ 1.5{\sim}3.5$ times larger than that of leaf, N content of the leaf ($4.48{\sim}5.27%$) was about 2 times higher than that of the stem ($2.00{\sim}3.62%$). High content of N might be responsible for the high growth rate of H. japonicus in summer. This result provides valuable information for appropriate timing for the removal of H. japonicus.

• Korean Journal of Food Science and Technology
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• v.26 no.4
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• pp.464-470
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• 1994

The biological activities of Humulus japonicus were extracted by water and methanol. Methanol was better solvent than water in the extraction for antimicrobial activities against six different species of bacteria and two yeasts. The methanol extract was systematically fractionated with various organic solvents which have different polarities. From the result of antimicrobial activities against six species of bacteria and two species of yeasts, methanol extract was superior to water extract. The methanol extract of Humulus japonicus showed antimicrobial activity against the all species of microorganisms tested except Escherichia coli . The butanol fraction of methanol extract showed antimicrobial effect on the all species tested. The minimal inhibition concentration(MIC) of the butanol fraction on the growth of microorganisms was ranged between $0.1{\sim}0.4%$. The water extract of Humulus japonicus did not show inhibition of the activity of trypsin but methanol extract showed inhibitory activity. The chloroform fraction of methanol extract showed comparatively higher trypsin inhibitory activity than other fractions. The concentration of 50% inhibition$(IC_{50})$ by chloroform fraction was 1.0 mg/ml. Enzyme-inhibitor complex formation was above 90% of the while for 20 min. It was revealed that methanol extract of Humulus japonicus inhibited peroxide production of lard and soybean oil as substrate by antioxidative test. The chloroform fraction of methanol extract had the highest activity. When 0.2% of chloroform fraction was added, induction period of soybean oil and lard were extended 15, 9 days, respectively.