Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Development and Evaluation of Non-Hydrous Skin Analogue Liquid Crystal using Thermo-Sensitivity Smart Sensor

  • Yoo, Kwang-Ho (Skin research institute, Korea Kolmar Corporation) ;
  • Hong, Jae-Hwa (Skin research institute, Korea Kolmar Corporation) ;
  • Eun, So-Hee (Skin research institute, Korea Kolmar Corporation) ;
  • Jeong, Tae-Hwa (Skin research institute, Korea Kolmar Corporation) ;
  • Jeong, Kwan-Young (Skin research institute, Korea Kolmar Corporation)
  • Received : 2014.06.30
  • Accepted : 2014.09.25
  • Published : 2014.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, skin permeation enhancement was confirmed by designing it to have a structure and composition similarity to the intercellular lipids that improve miscibility with skin by cross-linked lipids poloxamer. The cross-linked lipids poloxamer was synthesized and analyzed by 1H NMR that structure dose had conjugated pluronic with ceramide3. Active component is released by modification of liquid crystal structure because PPO part, large-scale molecule block of pluronic, has hydrophobic nature at skin temperature of $35^{\circ}C$. Conjugated pluronic with ceramide3 was synthesized using Pluronic F127 and p-NPC (4-nitrophenyl chloroformate) at room temperature yielded 89%. Pluronic(Ceramide 3-conjugated Pluronic) was synthesized by reaction of p-NP-Pluronic with Ceramide3 and DMAP. The yield was 51%. This cross-linked lipids poloxamer was blended and dissolved at isotropic state with skin surface lipids, phospholipid, ceramide, cholesterol and anhydrous additive solvent. Next step was preceded by ${\alpha}$-Transition at low temperature for making the structure of Meso-Phase Lamella, and non-hydrous skin analogue liquid crystal using thermo-sensitivity smart sensor, lamellar liquid crystal structure through aging time. For confirmation of conjugation thermo-sensitivity smart sensor and non-hydrous skin analogue liquid crystal, structural observation and stability test were performed using XRD(Xray Diffraction), DSC(Differential Scanning Calorimetry), PM (Polarized Microscope) And C-SEM (Cryo-Scanning Electron Microscope). Thermo-sensitivity observation by Franz cell revealed that synthesized smart sensor shown skin permeation effect over 75% than normal liquid crystal. Furthermore, normal non-hydrous skin analogue liquid crystal that not applied smart sensor shown similar results below $35^{\circ}C$ of skin temperature, but its effects has increased more than 30% above $35^{\circ}C$.

Keywords

References

  1. B. D. Park. M. J. Lee, J. K. Lee, S. H. Lee, The Preparation and Application of Lamella Liquid Crystal to Skin Care Product, J. Soc. Cosmet. Scientists Korea., 26(1), 93(2000).
  2. M. Chorilli, P. S. Prestes, R. B. Rigon, G.R. Leonardi, L. A. Chiavacci, V. H.Sarmento, A. G. Oliveira, M. V. Scarpa, Structural Characterization and In vivo Evaluation of Retinyl Palmitate in Non-ionic Lamellar Liquid Crystalline System, Colloids and Surfaces B:Biointerfaces., 85, 182(2011). https://doi.org/10.1016/j.colsurfb.2011.02.027
  3. E. C. Cho, H. J. Lim, J. W. Shim, J. O.Kim, I. S. Chang, Improved Stability of Liposome in Oil/water Emulsion by Association of Amphiphilic Polymer with Liposome and Its Effect on Bioactive Skin Permeation, Colloids and Surfaces A:Physicochem. Eng. Aspects., 299, 160 (2007). https://doi.org/10.1016/j.colsurfa.2006.11.032
  4. M. Kuentz., Oral Self-emulsifying Drug Delivery Systems, from Biopharmaceutical to Technical Formulation Aspects. J. Drug Del. Sci. Tech., 21(1), 17(2011). https://doi.org/10.1016/S1773-2247(11)50002-4
  5. Y. W. Choi, B. S. Jang, N. H. Jeong, Preparation and Properties of Collagen-Liposome using Hydrogenated Phosphatidylcholine, J Korean Oil Chem Soc., 29(2), 295(2012).
  6. A. J. Bevacqua, K. M. Lahanas, I. D.Cohen, G. Cioca, Liquid Crystals in Multiple Emulsions, Cosmetics &Toiletries., 106, 53(1991).
  7. R. Y. Lochhead, Emulsions, Cosmetic &Toiletries., 109, 94(1994).
  8. T. Gao, J. M. Tien, Y. H. Choi, Sunscreen Formulas with Multilayer Lamellar Structure, Cosmetics & Toiletries magazine., 118(10), 41(2003).
  9. S. Bekiranov, R. Bruinsma, P. Pinus, Phys. Rev. E., 55, 577(1997).
  10. E.E. Dormidontova, Macromolecules, 35, 987(2002). https://doi.org/10.1021/ma010804e
  11. D.C. Kannan, J.L. Duda, R.P. Danner, Fluid Phase Equilib., 237, 86(2005). https://doi.org/10.1016/j.fluid.2005.08.012
  12. S. Furyk, Y. Zhang, D. Ortiz-Acosta, P.S. Cremer, D.E. Bergbreiter, J. Polym. Sci. A., 44, 1492(2006). https://doi.org/10.1002/pola.21256
  13. Y. Nagasaki, F. Matsukura, M. Kato, H. Aoki, T. Tokuda, Macromolecules., 29, 5859(1996). https://doi.org/10.1021/ma960372l