KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Properties of Cellulase Immobilized on Chitosan Beads

키토산 비드에 고정화된 셀룰라아제의 특성

  • Lee, Sang Heon (Department of Chemical Engineering, College of Engineering, Chungbuk National University) ;
  • Ha, Yongil (Department of Chemical Engineering, College of Engineering, Chungbuk National University) ;
  • Kim, Bo Young (Department of Chemical Engineering, College of Engineering, Chungbuk National University) ;
  • Kim, Beom Soo (Department of Chemical Engineering, College of Engineering, Chungbuk National University)
  • 이상헌 (충북대학교 공과대학 화학공학과) ;
  • 하용일 (충북대학교 공과대학 화학공학과) ;
  • 김보영 (충북대학교 공과대학 화학공학과) ;
  • 김범수 (충북대학교 공과대학 화학공학과)
  • Received : 2014.05.08
  • Accepted : 2014.06.02
  • Published : 2014.08.31
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Abstract

Recently, there is a growing interest in efficient biomass pretreatment and saccharification processes to produce biofuels and biochemicals from renewable non-food biomass resources. In this study, glucose was produced from cellulose by immobilizing cellulase enzyme on chitosan beads which was reported to have high pH and temperature stability. The immobilized amounts of cellulase on chitosan beads linearly increased with increasing the concentrations of cellulase solution. The glucose production increased to 7.2 g/L from 1% carboxymethyl cellulose (CMC) substrate when immobilized at 20% cellulase solution. The maximum specific activity was 0.37 unit/mg protein when immobilized at 8% cellulase solution. At pH 7 and $37^{\circ}C$, the optimum reaction composition was 0.5 g beads/L from 1% CMC substrate. At this condition, the conversion to glucose completed at ca. 20 min.

Keywords

References

  1. Limayem, A., and S. C. Ricke (2012) Lignocellulosic biomass for bioethanol production: Current perspectives, potential issues and future prospects. Prog. Energ. Combust. 38: 449-467. https://doi.org/10.1016/j.pecs.2012.03.002
  2. Ra, C. H., H. J. Lee, M. K. Shin, and S. K. Kim (2013) Bioethanol production from seaweed Gelidium amansii for separated hydrolysis and fermentation (SHF). Korean Soc. Biotechnol. Bioeng. J. 28: 282-286. https://doi.org/10.7841/ksbbj.2013.28.5.282
  3. Kim, T. U. and E. K. Kim (2009) Bioin Issues & Specials Vol. 10, http://www.bioin.or.kr/board.do?num=195852&cmd=view&bid=feature.
  4. Kim, G. S., M. G. Sin, Y. J. Kim, J. J. Yun, and S. H. Kim (2009) Bioin Special WebZine Vol. 11, http://www.bioin.or.kr/board.do?num=188526&cmd=view&bid=report.
  5. Santos, A. M. P., M. G. Oliveira, and F. Maugeri (2007) Modelling thermal stability and activity of free and immobilized enzymes as a novel tool for enzyme reactor design. Bioresource Technol. 98: 3142-3148. https://doi.org/10.1016/j.biortech.2006.10.035
  6. Park, J. W. (2013) Carbon dioxide sequestration of enzyme covalently immobilized on porous membrane. Korean Soc. Biotechnol. Bioeng. J. 28: 225-229. https://doi.org/10.7841/ksbbj.2013.28.4.225
  7. Ahn, H. K., B. C. Kim, S. H. Jun, M. S. Chang, D. Lopez-Ferrer, R. D. Smith, M. B. Gu, S. W. Lee, B. S. Kim, and J. Kim (2010) Robust trypsin coating on electrospun polymer nanofibers in rigor-ous conditions and its uses for protein digestion. Biotechnol. Bioeng. 107: 921-927.
  8. Chang, R. H. Y., J. Jang, and K. C. W. Wu (2011) Cellulase immobilized mesoporous silica nanocatalysts for efficient cellulose-toglucose conversion. Green Chem. 13: 2844-2850. https://doi.org/10.1039/c1gc15563f
  9. Zang, L., J. Qiu, X. Wu, W. Zhang, E. Sakai, and Y. Wei (2014) Preparation of magnetic chitosan nanoparticles as support for cellulase immobilization. Ind. Eng. Chem. Res. 53: 3448-3454. https://doi.org/10.1021/ie404072s
  10. Khoshnevisan, K., A. K. Bordbar, D. Zare, D. Davoodi, M. Noruzi, M. Barkhi, and M. Tabatabaei (2011) Immobilization of cellulase enzyme on superparamagnetic nanoparticles and determination of its activity and stability. Chem. Eng. J. 171: 669-673. https://doi.org/10.1016/j.cej.2011.04.039
  11. Dincer, A. and A. Telefoncu (2007) Improving the stability of cellulase by immobilization on modified polyvinyl alcohol coated chitosan beads. J. Mol. Catal. B-Enzym. 45: 10-14. https://doi.org/10.1016/j.molcatb.2006.10.005
  12. Sung, I. K., J. Y. Song, and B. S. Kim (2011) Preparation of chitosan/poly-glutamic acid nanoparticles and their application to removal of heavy metals. Korean Chem. Eng. Res. 49: 475-479. https://doi.org/10.9713/kcer.2011.49.4.475
  13. Mao, X., G. Guo, J. Huang, Z. Du, Z. Huang, L. Ma, P. Li, and L. Gu (2006) A novel method to prepare chitosan powder and its application in cellulase immobilization. J. Chem. Technol. Biotechnol. 81: 189-195. https://doi.org/10.1002/jctb.1378
  14. Dave, R. and D. Madamwar (2006) Esterification in organic solvents by lipase immobilized in polymer of PVA-alginate-boric acid. Process Biochem. 41: 951-955. https://doi.org/10.1016/j.procbio.2005.10.019
  15. Mi, F. L., S. S. Shyu, S. T. Lee, and T. B. Wong (1999) Kinetic study of chitosan-tripolyphosphate complex reaction and acidresistive properties of the chitosan-tripolyphosphate gel beads prepared by in-liquid curing method. J. Polym. Sci. B: Polym. Phys. 37: 1551-1564. https://doi.org/10.1002/(SICI)1099-0488(19990715)37:14<1551::AID-POLB1>3.0.CO;2-H
  16. Zeng, X. and E. Ruckenstein (1998) Cross-linked macroporous chitosan anion-exchange membranes for protein separations. J. Membr. Sci. 148: 195-205. https://doi.org/10.1016/S0376-7388(98)00183-5