Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of Lactoferrin as a Human Dedifferentiation Factor Through the Studies of Reptile Tissue Regeneration Mechanisms

  • Bae, Kil Soo (Department of Biological Science, Dong-A University) ;
  • Kim, Sun Young (Research Center for Integrative Cellulomics, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Soon Yong (Department of Biological Science, Dong-A University) ;
  • Jeong, Ae Jin (Department of Biological Science, Dong-A University) ;
  • Lee, Hyun Hee (Department of Biological Science, Dong-A University) ;
  • Lee, Jungwoon (Regenerative Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Cho, Yee Sook (Regenerative Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Leem, Sun-Hee (Department of Biological Science, Dong-A University) ;
  • Kang, Tae-Hong (Department of Biological Science, Dong-A University) ;
  • Bae, Kwang-Hee (Research Center for Integrative Cellulomics, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Jae Ho (Medical Research Center for Ischemic Tissue Regeneration, Pusan National University) ;
  • Jung, Yong Woo (Department of Pharmacy, Korea University) ;
  • Jun, Woojin (Department of Food and Nutrition, Chonnam National University) ;
  • Yoon, Suk Ran (Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Sang-Chul (Research Center for Integrative Cellulomics, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chung, Jin Woong (Department of Biological Science, Dong-A University)
  • Received : 2014.02.05
  • Accepted : 2014.03.13
  • Published : 2014.06.28
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Abstract

In this study, we performed two-dimensional electrophoresis with protein extracts from lizard tails, and analyzed the protein expression profiles during the tissue regeneration to identify the dedifferentiation factor. As a result, we identified 18 protein spots among total of 292 spots, of which proteins were specifically expressed during blastema formation. We selected lactoferrin as a candidate because it is the mammalian homolog of leech-derived tryptase inhibitor, which showed the highest frequency among the 18 proteins. Lactoferrin was specifically expressed in various stem cell lines, and enhanced the efficiency of iPSC generation upto approximately 7-fold relative to the control. Furthermore, lactoferrin increased the efficiency by 2-fold without enforced expression of Klf4. These results suggest that lactoferrin may induce dedifferentiation, at least partly by increasing the expression of Klf4.

Keywords

References

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