Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Isolation of Polysaccharides Modulating Intestinal Immune System and Single Oral Dose Toxicity Test in Astragalus membranaceus Abovegroud Parts

황기 지상부로부터 장관면역 활성 다당체의 분리 및 단회 경구 투여 독성시험

  • Choi, Ri Na (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Park, Yeong Chul (GLP Center, Catholic University of Daegu) ;
  • Lee, Ji Sun (GLP Center, Catholic University of Daegu) ;
  • Kim, Jung Woo (GLP Center, Catholic University of Daegu) ;
  • Kim, Jong Bong (GLP Center, Catholic University of Daegu) ;
  • Cheoi, Yu Soon (Kangwon Jeongseon Agricultural Extension Center) ;
  • Kim, Kwang Ki (Kangwon Jeongseon Agricultural Extension Center) ;
  • Lee, Jae Geun (Department of Applied Plant Sciences, Kangwon National University) ;
  • Yu, Chang Yeon (Department of Applied Plant Sciences, Kangwon National University) ;
  • Kim, Seung Hyn (Department of Applied Bioscience, Konkuk University) ;
  • Chung, Ill Min (Department of Applied Bioscience, Konkuk University) ;
  • Kim, Jae Kwang (Division of Life Sciences, Incheon National University) ;
  • Lim, Jung Dae (Department of Herbal Medicine Resource, Kangwon National University)
  • 최리나 (강원대학교 생약자원개발학과) ;
  • 박영철 (대구 가톨릭대학교 GLP 센터 및 의생명학과) ;
  • 이지선 (대구 가톨릭대학교 GLP 센터 및 의생명학과) ;
  • 김정우 (대구 가톨릭대학교 GLP 센터 및 의생명학과) ;
  • 김종봉 (대구 가톨릭대학교 GLP 센터 및 의생명학과) ;
  • 최유순 (강원정선군농업기술센터) ;
  • 김광기 (강원정선군농업기술센터) ;
  • 이재근 (강원대학교 식물자원응용공학과) ;
  • 유창연 (강원대학교 식물자원응용공학과) ;
  • 김승현 (건국대학교 응용생물과학과) ;
  • 정일민 (건국대학교 응용생물과학과) ;
  • 김재광 (인천대학교 생명과학부) ;
  • 임정대 (강원대학교 생약자원개발학과)
  • Received : 2014.05.10
  • Accepted : 2014.06.26
  • Published : 2014.08.30
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Abstract

The six polysaccharide fractions were prepared by chromatographic procedure from the hot water extracts of the aboveground parts of Astragalus membranaceus. These six polysaccharides from aboveground parts of Astragalus membranaceus Bunge were tested for gut-mucosal immune activity and acute toxicity. In a view of molecular weight, the six fractions were estimated to be 75000, 88000, 129000 and 345000 Da, respectively. Component sugar analysis indicated that these fractions were mainly consisted of galactose (46.3 ~ 11.8%) and arabinose (35.4 ~ 9.9%) in addition to glucose, rhamnose, fucose, arabinose, xylose, mannose, glucuronic acid and galacturonic acid. Among the six major purified polysaccharides, AMA-1-b-PS2 showed highest bone merrow cell proliferation and lymphocyte of Peyer's patch stimulating activity. It may be concluded that intestinal immune system modulating activity of aboveground parts from Astragalus membranaceus Bunge is caused by polysaccharides having a polygalacturonan moiety with neutral sugars such as arabinose and galactose. In single oral dose toxicity study, no differences were observed between control and treated groups in clinical signs. The results indicated that lethal dose 50 ($LD_{50}$) of water extracts from Astragalus membranaceus-aboveground parts was found to be higher than 5000 mg/kg/day in this experiment. From the above results, we may suggest that Astragalus membranaceus-aboveground parts might have useful as a safe material for functional food and pharmaceutics.

Keywords

References

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