Advances in nano research

Techno-Press (테크노프레스)
권호 표지
DOI QR코드

DOI QR Code

A novel method for the synthesis of nano-magnetite particles

  • Received : 2013.06.22
  • Accepted : 2014.03.28
  • Published : 2014.06.25
⟨ Previous Next ⟩

Abstract

A novel and simple method for the synthesis of nano-magnetite particles is disclosed. In the novel procedure, $Fe^{2+}$ is the only source of metal cation. Carboxymethylcellulose (CMC) is used as the structure directing agent. The phase analysis of the nano-particles was performed using XRD and electron diffraction techniques. Size and morphology analysis was performed using light scattering and TEM techniques. The effect of $NH_4OH$ solution (32 wt. %) at different CMC concentrations on the size distribution of the final magnetite powders is studied. An optimal base concentration exists for each CMC concentration leading to minimal agglomeration. There exists a minimum CMC concentration (0.0016 wt. %), lower than that no magnetite forms. It is shown that using the new method, it is possible to immobilize a lipase enzyme (Candida Rugosa) with immobilization efficiency larger than 98 % with a loading more than 3 times the reported value in the literature. The latter phenomenon is explained based on the agglomerate state of the nano-particles in the liquid phase.

Keywords

References

  1. Tartaj, P., del Puerto Morales, M., Veintemilas-verdanguer Gonzales-Carreno, T. and Serna, C. (2003), "The preparation of magnetic nano-particles for applications in biomedicine", J. Phys. D, Appl., Phys., 36, R182-R197. https://doi.org/10.1088/0022-3727/36/13/202
  2. Liu, X., Guan, Y., Shen, R. and Liu, H. (2005), "Immobilization of lipase onto micron-size magnetic beads", J. Chrom. B, 822, 91-97. https://doi.org/10.1016/j.jchromb.2005.06.001
  3. Huang, S.H., Liao, M.H. and Chen, D.H. (2003), "Direct binding and characterization of lipase onto magnetic nanoparticles", J. Biotechnol. Prog., 19, 1095-1100. https://doi.org/10.1021/bp025587v
  4. Liu, X., Guan, Y., Shen, R. and Liu, H. (2005), "Immobilization of lipase onto micron-size magnetic beads", J. Chromatography B, 822, 91-97. https://doi.org/10.1016/j.jchromb.2005.06.001
  5. Cheng, F.Y., Su, C.H. and Yag, Y.S. (2005), "Characterization of aqoues dispersions of $Fe_{3}O_{4}$ nanoparticles and their biomedical applications", J. Biomaterials, 26, 279-238.
  6. Rabelo, D., Lima, E.C.D., Reis, A.C., Nures, W.C., Novak, M.A., Garg, V.K., Oliveira, A.C. and Morais, P.C. (2001), "Preparation of magnetite nano-particles in meso-porous copolymer template", Nanoletters, 1, 105-108. https://doi.org/10.1021/nl005533k
  7. Kumar, R.V., Koltypin, Y., Xu, X.N., Yeshurun, Y., Gedankan, A. and Felner, I. (2001), "Fabrication of magnetite nano-rods by ultrasound irradiation", J. Appl. Phys., 89, 6324-6328. https://doi.org/10.1063/1.1369408
  8. Sipos, P. (2003), "Manufacturing of size controlled magnetite nanoparticles potentially suitable for the preparation of aqueous magnetic fluids", Rom. Report. Phys., 58(3), 269-272.
  9. Wibowo, N., Yang, P.F. and Lee, C.K. (2013), "Epsilon-polysine fermentation and its recovery using commercial cellulose (CMC)-conjugated magnetite", Sep. Sci. Technol., 48, 1086-1092. https://doi.org/10.1080/01496395.2012.723099

Cited by

  1. Elastic wave dispersion modelling within rotating functionally graded nanobeams in thermal environment vol.6, pp.3, 2014, https://doi.org/10.12989/anr.2018.6.3.201