식물분류학회지 (Korean Journal of Plant Taxonomy)

  • 제44권2호
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  • Pages.111-118
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  • 2014
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  • 1225-8318(pISSN)
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  • 2466-1546(eISSN)
한국식물분류학회 (The Korean Society of Plant Taxonomists)
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엽록체 DNA psbA-trnH와 atpF-H 염기서열에 기초한 한국산 소나무속의 분자계통학적 연구

Molecular phylogenetic study of Pinus in Korea based on chloroplast DNA psbA-trnH and atpF-H sequences data

  • 투고 : 2014.05.13
  • 심사 : 2014.06.13
  • 발행 : 2014.06.30
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초록

엽록체 DNA atpF-H, psbA-trnH region을 마커로 활용하여 국내에 분포하는 소나무속 식물들 중 17분류군에 대한 분자계통학적 연구를 수행하여 한국산 소나무속의 계통학적 유연관계를 규명하고, 소나무속의 유연관계를 잘 나타낼 수 있는 분자마커를 찾아내고자 연구가 수행되었다. atpF-H, psbA-trnH region의 조합분석결과 한국산 소나무속은 100%의 BP로 지지되는 단계통군으로 확인되었으며, 소나무아속과 잣나무아속으로 명확히 구분되어졌다. 본 연구에서 이용된 두 개의 분자마커 중 psbA-trnH region이 atpF-H region보다 한국산 소나무속의 계통 및 유연관계를 규명하는데 다소 높은 해상력을 나타내었다.

This study aims to define the phylogenetic relationship within Korean Pinus L. and to find the molecular markers which resolve the phylogenetic relationship in genus Pinus. cpDNA atpF-H and psbA-trnH regions were used as molecular markers. We performed the molecular phylogenetic study on 17 taxa of Pinus in Korea. The combined analyses of two gene loci showed that Korean Pinus was a monophyletic group supported by 100% BP. According to the results of separate analyses, psbA-trnH region seems to work better resolving power to clarify the phylogenetic ambiguity in Korean Pinus than those of atpF-H region. Also, we tried to checked the value and resolution of two chloroplast DNA loci on phylogenetic implications.

키워드

참고문헌

  1. CBOL Plant Working Group. 2009. A DNA barcode for land plants. Proceedings of the National Academy of Sciences of the United States of America 106 (31): 12794-12797. https://doi.org/10.1073/pnas.0905845106
  2. Chaw, S. M., A. Zharkikh, H. M. Sung, T. C. Lau and W. H. Li. 1997. Molecular Phylogeny of Extant Gymnosperms and Seed Plant Evolution: Analysis of Nuclear 18S rRNA Sequences. Molecular Biology and Evolution 14: 56-68. https://doi.org/10.1093/oxfordjournals.molbev.a025702
  3. Chaw, S. M., C. L. Parkinson, Y. Cheng, T. M. Vincent and J. D. Palmer. 2000. Seed plant phylogeny inferred from all three plant genomes: Monophyly of extant gymnosperms and origin of Gnetales from conifers. Proc. Proceedings of the National Academy of Sciences of the United States of America 97 (8): 4086-4091. https://doi.org/10.1073/pnas.97.8.4086
  4. Fu, L-K., L. Nan, and R. R. Mill. 1999. Flora of China. Vol. 4. Science Press, Beijing, and Missouri Botanical Garden Press, St. Louis. Pp. 11-25.
  5. Gernandt, D. S., G. Lopez, S. O. Garcia and A. Liston. 2005. Phylogeny and classification of Pinus. Taxon 54: 29-42. https://doi.org/10.2307/25065300
  6. Helander, J. 2000. Phylogeny of Gymnosperms: Fact and Fiction. Gymnosperms, Gnetales, Coniferales, Pinaceae, Pinus, Abies. Poul Mollers Vej 7, Copenhagen.
  7. Kim, H. J. 2008. Systematic Studies on Stomatal Type of Genus Pinus. MS thesis. Sangji University. (in Korean)
  8. Kim, J. H. D.-K. Kim and J.-H. Kim. 2009. Genetic variation and relationships of Artemisia capillaris Thunb. (Compositae) by RAPD analysis. Korean Journal of Plant Resources 22 (3): 242-247. (in Korean)
  9. Kim, J. U. and B. S. Kil. 1983. A Study on the Distribution of Pinus thunbergii in the Korean Peninsula. Journal of Ecology and Environment 6(1): 45-54. (in Korean)
  10. Kim, Y.-H., K.-H. Tae and J.-H. Kim. 2008. Genetic variations and relationships of Persicaria thunbergii (Sieb. & Zucc.) H. Gross ex Nakai (Polygonaceae) by the RAPD analysis. Korean Journal of Plant Resources 21(1): 66-72. (in Korean)
  11. Kim, Y. S., S. C. Ko, and B. H. Choi. 1981. Distribution Atlas of plants of Korea (4) Atlas of Pinaceae in Korea. Korean Journal of Plant Taxonomy 11(1,2): 53-75. (in Korean)
  12. Korea National Arboretum and The Plant Taxonomic Society of Korea. 2007. A Synonymic List of Vascular Plants in Korea. Korea National Arboretum. Pocheon. (in Korean)
  13. Lee, T. B. 2003. Coloured Flora of Korea. Hyangmunsa. (in Korean)
  14. Lee, T. B. 2004. (New) Dendrology. Hyangmunsa. (in Korean)
  15. Lee, T. B. 2008. (New) Plant Taxonomy. Hyangmunsa. (in Korean)
  16. Lee, Y. N. 1986. Coniferopsida in Korea. Ewha Womans University Press. (in Korean)
  17. Min, J. K. 1995. Systematic studies on Pinaceae 6 genera in Korea. MS thesis. Konkuk University. (in Korean)
  18. Nakai, T. 1909. Flora koreana. Pars prima. Journal of the College of Science, Imperial University of Tokyo 26: 1-304.
  19. Nakai, T. 1911. Notulae ad plantas Japoniae et Koreae. Botanical Magazine Tokyo 25: 52-64. https://doi.org/10.15281/jplantres1887.25.289_52
  20. Richardson, D.M. 1998. Ecology and Biogeography of Pinus. Cambridge University Press.
  21. Syring, J., A. Willyard, R. Cronn and A. Liston. 2005. Evolutionary relationships among Pinus (Pinaceae) subsections inferred from multiple low-copy nuclear loci. American Journal of Botany 92: 2086-2100. https://doi.org/10.3732/ajb.92.12.2086
  22. Uyeki, H. 1926. Corean Timber Trees, vol. 1. Ginkgoales and Coniferae. The Forestry Experiment Station, Government General of Chosen. (in Japanese)
  23. Wang, X. R., Y. Tsumura, H. Yoshimaru, K. Nagasaka and A. E. Szmidt. 1999. Phylogenetic relationships of Eurasian pines (Pinus, Pinaceae) based on chloroplast rbcL, matK, rpl20-rps18 spacer, and trnV intron sequences. American Journal of Botany 86: 1742-1753. https://doi.org/10.2307/2656672
  24. Wang, X.-Q., D. C. Tank, and T. Sang. 2000. Phylogeny and divergence times in Pinaceae: evidence from three genomes. Molecular Biology and Evolution 17: 773-781. https://doi.org/10.1093/oxfordjournals.molbev.a026356
  25. White, T. J., T. D. Bruns, S. Lee and J. W. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications. Innis, M. A., D. H. Gelfand, J. J. Sninsky and T. J. White (eds), Academic Press, New York. Pp. 315-322.
  26. Yim, K. B. and K. J. Lee. 1978. The variation of natural population of Pinus densiflora S. et Z. in Korea, (VII) Characteristics of needle and wood of Wangsan, Bonghwa and Yangju Populations. Journal of Korean Forest Society 40: 1-18. (in Korean)
  27. Yim, K. B. and K. J. Lee. 1979. The variation of natural population of Pinus densiflora S. et Z. in Korea, (IX) Needle and wood characteristics of six populations. Journal of Korean Forest Society 43: 20-30. (in Korean)
  28. Yim, K. B. and K. W. Kwon. 1976a. The Variation of Natural Population of Pinus densiflora S. et Z. in Korea (II) -Characteristics of Needle and Wood of Myong-Ju, Ul-Jin, and Suweon Populations-. Journal of Korean Forest Society 31: 8-20. (in Korean)
  29. Yim, K. B. and K. W. Kwon. 1976b. The variation of natural population of Pinus densiflora S. et Z. in Korea, (III) Genetic variation of the progeny originated from Mt. Chu-wang, An-Myon Island and Mt. O-Dae Populations. Journal of Korean Forest Society 32: 36-63. (in Korean)
  30. Yim, K. B. and Z. S. Kim. 1975. The variation of natural population of Pinus densiflora S. et Z. in Korea, (I) Characteristics of needle and wood of Chuwang-san, An-Myeon-do and Odae-san Populations. Journal of Korean Forest Society 28: 1-20. (in Korean)
  31. Yim, K. B., K. W. Kwon and K. J. Lee. 1977. The variation of natural population of Pinus densiflora S. et Z. in Korea, (V) Characteristics of needle and wood of Injye, Jeongsun, Samchuk Populations. Journal of Korean Forest Society 36: 9-25. (in Korean)

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  2. Method of DNA Extraction from Pinus rigida Wood Pretreated with Sandpaper vol.46, pp.4, 2018, https://doi.org/10.5658/wood.2018.46.4.402
  3. Technical note: Polyvinylpyrrolidone (PVP) and proteinase-K improve the efficiency of DNA extraction from Japanese larch wood and PCR success rate vol.328, pp.None, 2014, https://doi.org/10.1016/j.forsciint.2021.111005