Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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The House dust Mite Allergen, Dermatophagoides pteronyssinus Regulates the Constitutive Apoptosis and Cytokine Secretion of Human Eosinophils

  • Kang, Bo Kyeong (Department of Biomedical Laboratory Science, School of Medicine, Eulji University) ;
  • Kim, A Min (Department of Biomedical Laboratory Science, School of Medicine, Eulji University) ;
  • Park, Sun Hwa (Department of Biomedical Laboratory Science, School of Medicine, Eulji University) ;
  • Lee, Eun Ji (Department of Biomedical Laboratory Science, School of Medicine, Eulji University) ;
  • Kim, Jung Seok (Department of Biomedical Laboratory Science, School of Medicine, Eulji University) ;
  • Kim, Eun Jeong (Department of Senior Healthcare, BK21 plus program, Graduated School, Eulji University) ;
  • Baek, Seung Yeop (Department of Senior Healthcare, BK21 plus program, Graduated School, Eulji University) ;
  • Kim, In Sik (Department of Biomedical Laboratory Science, School of Medicine, Eulji University)
  • Received : 2014.03.18
  • Accepted : 2014.03.25
  • Published : 2014.03.31
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Abstract

Asthma is an allergic inflammation and house dust mite (HDM) is a major allergen to induce asthma pathogenesis. Regulation of eosinophil apoptosis is an essential immune process and its dysregulation is implicated in asthma. In the present study, we examined the effects of HDM on spontaneous apoptosis of asthmatic eosinophils and on cytokine secretion in eosinophils of normal subjects including non-atopic and atopic normal. Extract of Dermatophagoides pteronissinus (DP) inhibited eosinophil apoptosis in a time-dependent manner. DP increased the secretion of G-CSF, GM-SCF, and IL-4, which is involved in suppression of eosinophil apoptosis, but IL-5 expression was not altered after DP stimulation. DP also elevated the release of IL-6, IL-8, tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and CCL2, which are anti-apoptotic or survival factors. The secretion of G-CSF, GM-CSF, IL-6, IL-8, and TNF-${\alpha}$ due to DP is higher in atopic normal than that in non-atopic normal. In conclusion, DP increases the survival of eosinophils and its mechanism may be associated with cytokine release. These findings may enable elucidation of asthma pathogenesis induced by HDM.

Keywords

References

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