Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
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Bone Healing Properties of Autoclaved Autogenous Bone Grafts Incorporating Recombinant Human Bone Morphogenetic Protein-2 and Comparison of Two Delivery Systems in a Segmental Rabbit Radius Defect

  • Choi, Eun Joo (Department of Oral and Maxillofacial Surgery, Yonsei University College of Dentistry) ;
  • Kang, Sang-Hoon (Department of Oral and Maxillofacial Surgery, Yonsei University College of Dentistry) ;
  • Kwon, Hyun-Jin (Department of Oral Biology, Yonsei University College of Dentistry) ;
  • Cho, Sung-Won (Department of Oral Biology, Yonsei University College of Dentistry) ;
  • Kim, Hyung Jun (Department of Oral and Maxillofacial Surgery, Yonsei University College of Dentistry)
  • Received : 2014.04.02
  • Accepted : 2014.05.14
  • Published : 2014.05.31
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Abstract

Purpose: This study aims to validate the effect of autoclaved autogenous bone (AAB), incorporating Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2), on critical-sized, segmental radius defects in rabbits. Delivery systems using absorbable collagen sponge (ACS) and fibrin glue (FG) were also evaluated. Methods: Radius defects were made in 12 New Zealand white rabbits. After autoclaving, the resected bone was reinserted and fixed. The animals were classified into three groups: only AAB reinserted (group 1, control), and AAB and ErhBMP-2 inserted using an ACS (group 2) or FG (group 3) as a carrier. Animals were sacrificed six or 12 weeks after surgery. Specimens were evaluated using radiology and histology. Results: Micro-computed tomography images showed the best bony union in group 2 at six and 12 weeks after operation. Quantitative analysis showed all indices except trabecular thickness were the highest in group 2 and the lowest in group 1 at twelve weeks. Histologic results showed the greatest bony union between AAB and radial bone at twelve weeks, indicating the highest degree of engraftment. Conclusion: ErhBMP-2 increases bony healing when applied on AAB graft sites. In addition, the ACS was reconfirmed as a useful delivery system for ErhBMP-2.

Keywords

References

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