Journal of Biosystems Engineering

  • 제39권2호
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  • Pages.122-133
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  • 2014
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  • 1738-1266(pISSN)
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  • 2234-1862(eISSN)
한국농업기계학회 (Korean Society for Agricultural Machinery)
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Development and Characterization of Horse Bone-derived Natural Calcium Phosphate Powders

  • Jang, Kyoung-Je (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Cho, Woo Jae (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Seonwoo, Hoon (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Kim, Jangho (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Lim, Ki Taek (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Chung, Pill-Hoon (Department of Oral and Maxillofacial Surgery and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Chung, Jong Hoon (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University)
  • 투고 : 2014.05.16
  • 심사 : 2014.05.27
  • 발행 : 2014.06.01
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초록

Purpose: This study was to develop an effective process for fabricating biocompatible calcium phosphate powders (CPPs) using horse bones, and to investigate the characteristics of them. Methods: The characteristics of horse bone powders (HBPs) were investigated according to the different osseous tissue types (compact bone and cancellous bone), bone types (spine and tibia), pretreatment methods (cold water, $H_2O_2$, and hot water), sintering time (4, 8 and 12h), and sintering temperature (600, 900, 1100 and $1300^{\circ}C$). In addition, the grinding methods were compared based on the wet grinding (ball mill) and dry grinding (blade grinder) method to make it as powders. Finally, their cytotoxicity and cell viability were checked. Results: Regardless of the types of osseous tissues and bones, HBPs were well fabricated as biocompatible CPPs. It was also found that the pretreatment methods did not influence on the resultants, showing well-fabricated HBPs. Considering the processing time, the hot water method was the most suitable compared to other pretreatment methods. Further, 12h-sintering time was sufficient to remove residual organic compounds. The sintering temperatures greatly affected the properties of bone powders fabricated. The x-ray diffraction (XRD) peak of horse bone sintered at $600^{\circ}C$ was most closed to that of hydroxyapatite (HA). Our bioactivity study demonstrated that the HBPs fabricated by sintering horse bones at $1300^{\circ}C$ showed the best performance in terms of cell viability whereas the HBPs $1100^{\circ}C$ showed the cytotoxicity. Conclusions: Using various types of horse bone tissues, biocompatible CPPs were successfully developed. We conclude that the HBPs may have a great potential as biomaterials for various biological applications including bone tissue engineering.

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피인용 문헌

  1. Development and characterization of fast-hardening composite cements composed of natural ceramics originated from horse bones and chitosan solution vol.11, pp.5, 2014, https://doi.org/10.1007/s13770-014-0036-5