Journal of Plant Biotechnology

  • 제41권1호
  • /
  • Pages.38-43
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  • 2014
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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멸종위기종 둥근잎꿩의비름 (Hylotelephium ussuriense (Kom.) H. Ohba)의 기 내 증식

In vitro propagation of endangered species, Hylotelephium ussuriense (Kom.) H. Ohba

  • 배기화 (국립생물자원관) ;
  • 유경화 (공주대학교 자연과학대학 생명과학과) ;
  • 김지아 (국립산림과학원 산림생명공학과) ;
  • 윤의수 (공주대학교 자연과학대학 생명과학과)
  • Bae, Kee-Hwa (National Institute of Biological Resources) ;
  • Yoo, Kyoung-Hwa (Department of Biology, College of Nature Sciences, Kongju National University) ;
  • Kim, Ji-Ah (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Yoon, Eui-Soo (Department of Biology, College of Nature Sciences, Kongju National University)
  • 투고 : 2014.02.28
  • 심사 : 2014.03.11
  • 발행 : 2014.03.31
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초록

멸종위기수종인 둥근잎꿩의비름 기내 부정아유도에 미치는 식물생장조절물질의 종류 및 배양부위의 영향과 기내생육 및 엽록소함량에 미치는 배지, sucrose 농도 및 배양용기에 따른 환기효과를 연구하였다. 부정아 유도는 3.0 m g/L의 BA와 0.01 mg/L의 IBA가 첨가된 배지에 액아포함 줄기를 배양하였을 때 가장 효과적이었다. 기내 부정아의 줄기와 뿌리 신장에 MS배지의 농도와 sucrose의 농도는 영향을 주지 않았다. 환기처리가 기내 건전 식물체 생산에 미치는 영향을 조사한 결과, 기내 환기처리구는 줄기신장에 효과적이었고, 뿌리의 신장은 유의적 차이는 없이 모든 실험구에서 10 cm 이상 증식하였다. 엽록소의 함량은 환기처리 시 총엽록소 함량이 3.12 mg/g으로 높게 나타남을 확인하였다. 둥근잎꿩의비름의 부정아 유도에 농도와 배양부위가 중요한 요인으로 작용하고 기내식물체 신장에 미치는 배지 및 sucrose농도의 영향은 크지 않은 것으로 나타났다. 기내 건전식물체의 생산은 환기가 용이한 배양용기하에서 양호한 것으로 나타났다.

To establish the system of in vitro plant regeneration, the different explants (stem with axillary bud and stem without axillary bud) of Hylotelephium ussuriense were cultured on the Murashige and Skoog's medium containing 6-benzylaminopurine (BA) and indolebutyric acid (IBA). The adventitious shoot induction was more effective in the stem with axillary bud explants than the stem without axillary bud explants, and was the best on MS medium containing 3.0 mg/L BA and 0.01 mg/L IBA. Frequency of plantlet growth was not significantly treated on MS and sucrose. Total chlorophyll contents under ventilation treatment were higher than those in control (non-ventilation). This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.

키워드

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피인용 문헌

  1. Flora Distributed in Juwangsan National Park, Korea vol.19, pp.3, 2016, https://doi.org/10.13087/kosert.2016.19.3.71