• Journal of Plant Biotechnology
• /
• v.41 no.1
• /
• pp.38-43
• /
• 2014

To establish the system of in vitro plant regeneration, the different explants (stem with axillary bud and stem without axillary bud) of Hylotelephium ussuriense were cultured on the Murashige and Skoog's medium containing 6-benzylaminopurine (BA) and indolebutyric acid (IBA). The adventitious shoot induction was more effective in the stem with axillary bud explants than the stem without axillary bud explants, and was the best on MS medium containing 3.0 mg/L BA and 0.01 mg/L IBA. Frequency of plantlet growth was not significantly treated on MS and sucrose. Total chlorophyll contents under ventilation treatment were higher than those in control (non-ventilation). This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.

• Journal of Environmental Science International
• /
• v.22 no.7
• /
• pp.873-884
• /
• 2013

This study was carried out to establish the conservation plan of specific plants by surveying and analyse the individuals and the environment of habitats on specific plants designated by Juwangsan national park office. The specific plants were 14 species including Berchemia berchemiaefolia, Hylotelephium ussuriense, Aristolochia manshuriensis, Lilium cernuum, Thalictrum coreanum, Exochorda serratifolia, Lilium distichum, Aristolochia contorta, Jeffersonia dubia, Eleutherococcus senticosus, Salix chaenomeloids, Eranthis stellata, Scopolia japonica, and Paeonia japonica. The habitats of specific plants were 40 sites including Berchemia berchemiaefolia of 11 sites, Hylotelephium ussuriense of 8 sites, Lilium cernuum and Thalictrum coreanum of each 3 sites, Exochorda serratifolia of 2 sites, and Lilium distichum, Aristolochia contorta, Jeffersonia dubia, Eleutherococcus senticosus, Salix chaenomeloids, Eranthis stellata, Scopolia japonica and Paeonia japonica of each 1 site. The altitude was the most in 300~400 m. In direction, NW was the most, and it showed SW in that order. The slope angle was the most in 0~10%, and the cliff showed in that order. In the results of species composition by stratum layer, Berchemia berchemiaefolia had the most, 15 species, in tree layer. In shrub and herb layer, this had the most as 20 species and 29 species, too. The species that occupied the largest size was Salix chaenomeloids in 2,500 $m^2$, and Jeffersonia dubia showed 1,500 $m^2$ in that order. The conservation of specific plants is to accomplish the ex situ and in situ conservation at the same time.

• Korean Journal of Plant Taxonomy
• /
• v.36 no.4
• /
• pp.293-308
• /
• 2006

Genetic and morphological studies using morphological and isozymic data from 20 populations of 6 Korea Hylotelephium were conducted to resolve the species relationships and the origin of polyploid species. The results of morphological studies suggest that triploid H. viviparum is closely related to H. verticillatum, and the high frequency alleles of two species were very similar each other in isozyme analyses. Unique fixation of $PGI-1^b$ in two species supports that H. verticillatum should be the diploid ancestor of H. viviparum. However, a unique $ALD-1^c$ allele is present in H. viviparum, and the lack of the common alleles of H. erythrostictum and H. spectabile in this species eliminated them from the candidates of another tetraploid ancestor of H. viviparum. Based on the high morphological and genetic similarities, our results supported that the Korea endemic H. viridescens might be originated from the H. erythrostictum or share a common ancestor with it. Besides, H. ussuriense is closely related with H. erythrostictum and H. viridescens.

• Korean Journal of Plant Taxonomy
• /
• v.46 no.4
• /
• pp.361-370
• /
• 2016

An area comprising Juwangsan National Park and its adjacent mountains (southeastern Korean Peninsula) is a good model system for testing the effects of physical barriers to gene flows in plant populations. We predicted that plant species consisting of isolated populations are genetically more differentiated than those that are rather continuously distributed. Most populations of Sedum polytrichoides occur in four isolated valleys, and we assessed the genetic variability and structures using twelve allozyme loci in ten populations. We also compared the present results to earlier findings pertaining to the two co-occurring herbs Hylotelephium ussuriense (${\equiv}$ Sedum ussuriense) (growing only in the four isolated valleys) and S. kamtschaticum (rather continuously distributed). We found moderate levels of within-population genetic variation in S. polytrichoides ($H_{e}=0.112$). Estimates of among-population divergence in S. polytrichoides were also moderate ($F_{ST}=0.250$) and, as expected, very similar to that of H. ussuriense (0.261) but considerably higher than the variation in S. kamtschaticum (0.165). An analysis of molecular variance (AMOVA) revealed that S. polytrichoides and H. ussuriense had higher percentages of among-valley variation (19% each) than S. kamtschaticum (4%). Most of this variation, as also indicated by the STRUCTURE program, was due to differences in genetic profiles between the two central valleys. We concluded that the genetic differences observed between species (S. kamtschaticum vs. S. polytrichoides and H. ussuriense) are mainly due to differences in their distribution within the study area.

• Journal of Environmental Science International
• /
• v.30 no.11
• /
• pp.891-905
• /
• 2021

The aim of this study was to investigate the plants in Yongjibong Natural Recreation Forest before construction, to provide basic data that can be used for various purposes. The survey was conducted on the planned recreational forest sites from June to September 2020. The numbers of flora are summarized as follows: 348 taxa, 95 families, 234 genera, 308 species, three subspecies, 30 varieties, and seven forma. The Korean endemic plants were of nine taxa, including Fagus engleriana, Celtis choseniana, and Broussonetia x kazinoki. The rare plants were of six taxa, including Juniperus chinensis var. sargentii, Hylotelephium ussuriense, Thuja orientalis, and F. engleriana; however, Juniperus chinensis var. sargentii, H. ussuriense, T. orientalis, and Iris ensata var. spontanea were planted species. The floristic regional indicator plants (unique plants) found in this survey area were of 33 taxa, comprising three taxa of grade V, four taxa of grade IV, nine taxa of grade III, four taxa of grade II, and 13 taxa of grade I. The naturalized plants were of 26 taxa, including Rumex crispus, Phytolacca americana, and P. esculenta. Among the naturalized plants, it is necessary to prevent the spread of Ambrosia trifida, Bidens frondosa, and Tagetes minuta through continuous monitoring.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.19 no.3
• /
• pp.71-91
• /
• 2016

The purpose of this study is to offer the raw data for conservation of natural resources by surveying the flora distributed in Juwangsan National Park. The numbers of flora were summarized as 575 taxa; 99 families, 333 genera, 507 species, 4 subspecies, 56 varieties and 8 forms. The threatened species was Eleutherococcus senticosus, and the rare plants were 17 taxa; Anaphalis sinica, Jeffersonia dubia, Hylotelephium ussuriense, Berchemia berchemiaefolia and so forth. The Korean endemic plants were 13 taxa; Salix pseudolasiogyne, Carpinus laxiflora, Pseudostellaria coreana, Thalictrum actaefolium var. brevistylum, Corydalis albipetala and so forth. The specific plants by floristic region were 69 taxa; 31 taxa of grade I, 16 taxa of grade II, 8 taxa of grade III, 7 taxa of grade IV and 7 taxa of grade V. The naturalized plants were 27 taxa; Fallopia dumetorum, Rumex crispus, Chenopoidum album, Amaranthus patulus, Lepidium apetalum and so forth, and the invasive alien plants were Ambrosia artemisiifolia and Aster pilosus. The target plants adaptable to climate change were 18 taxa; Anemone reflexa, Eranthis stellata, Hylomecon vernalis, Lathyrus vaniotii and so forth. The plants with approval for delivering oversea were 31 taxa; Dianthus longicalyx, Thalictrum ichangense, Spiraea blumei, Glycine soja and so forth.

• Korean Journal of Environment and Ecology
• /
• v.25 no.4
• /
• pp.498-515
• /
• 2011

The flora of Juwangsan National Park was surveyed. The field works was done for seven times from March to October 2008. Endangered species designated by The Ministry of Environment in Juwangsan National Park were found to be Hylotelephium ussuriense (KOM.) H. OHBA, Berchemia berchemiaefolia (MAK.) KOIDZ, Jeffersonia dubia BENTH., Iris odaesanensis Y.N.LEE and Acanthopanax senticosus (RUPR. et MAX.) HARMS, Lilium cernum KOM., Thalictrum coreanum LEV.(7 taxa). The floristic special plants were recorded to a total of 88 taxa; that is, degree V species (8 family 9 taxa) were containing as Jeffersonia dubia BENTH., Berchemia berchemiaefolia (MAK.) KOIDZ, Lilium cernum KOM., etc, degree IV species (4 family 5 taxa) were Cimicifuga heracleifolia KOM., Anemone reflexa STEPH. et WILLD., Patrinia rupestris JUSS., etc, degree III species (16 family 18 taxa) were containing; Pyrrosia petiolosa (CHRIST.et BARONI) CHING, Exochorda serratifolia S. MOORE., Aristolochia manshuriensis KOM., etc, degree II species (14 family 18 taxa) were Paeonia japonica MIYABE et TAKEDA, Isopyrum raddeanum (REGEL) MAX., Cypripedium macramthum SW., etc and degree I species (30 family 38 taxa) were Botrychium virginianum (L.) SW., Chloranthus japonicus SIEB., Dictamnus dasycarpus TURCZ., etc. 15 taxa that included Salix hulteni FLODERUS, Celtis choseniana NAKAI, Clematis trichotoma NAKAI, Vicia venosissima NAKAI, etc. were found to be endemic to korea and 21 taxa that included ; Bilderdykia dumetora (L.) DUM., Bilderdykia convolvulus DUM., Lepidium apetalum WILLD., Amorpha fruticosa L. and Symphytum offcinale L., Hibiscus trionum L. etc. were identified as naturalized plants.

• Korean Journal of Plant Taxonomy
• /
• v.46 no.4
• /
• pp.371-377
• /
• 2016

The succulent herbaceous perennial Sedum sarmentosum commonly grows in the southern part of the Korean Peninsula. It is a species native to China, most likely introduced into Korea due to its edible and medicinal uses. If plants were introduced from a single source, we would expect no or low levels of genetic variation in Korean populations. Alternatively, if plants were introduced from multiple sources, we would expect, in contrast, high levels of genetic diversity. To test which is more likely, we surveyed the degree of allozyme variation in ten populations of this species from southern Korea. We found that S. sarmentosum was monomorphic at all fifteen allozyme loci. However, two congeners (S. polytrichoides and S. kamtschaticum) and two related species (Hylotelephium ussuriense and H. verticillatum) maintain moderate to high levels of genetic diversity ($H_e=0.144$, 0.203, 0.201, and 0.204, respectively). We suggest that southern Korean populations of S. sarmentosum likely descended from a single introduction of a few plants and then became naturalized exclusively via vegetative spreading (as plants in Korea, but also as occurs in other parts of its native and naturalized range, are sterile).