Biomaterials and Biomechanics in Bioengineering

  • 제1권1호
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  • Pages.27-39
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  • 2014
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  • 2465-9835(pISSN)
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  • 2465-9959(eISSN)
테크노프레스 (Techno-Press)
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DOI QR코드

DOI QR Code

Islet function within a multilayer microcapsule and efficacy of angiogenic protein delivery in an omentum pouch graft

  • McQuilling, J.P. (Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine) ;
  • Pareta, R. (Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine) ;
  • Sivanandane, S. (Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine) ;
  • Khanna, O. (Pritzker Institute of Biomedical Engineering, Illinois Institute of Technology) ;
  • Jiang, B. (Pritzker Institute of Biomedical Engineering, Illinois Institute of Technology) ;
  • Brey, E.M. (Pritzker Institute of Biomedical Engineering, Illinois Institute of Technology) ;
  • Orlando, G. (Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine) ;
  • Farney, A.C. (Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine) ;
  • Opara, E.C. (Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine)
  • 투고 : 2013.10.23
  • 심사 : 2014.02.19
  • 발행 : 2014.03.25
⟨ 이전 논문 다음 논문 ⟩

초록

We have previously described a new multilayer alginate microcapsule system, and the goals of the present study were to assess the in vitro function of islets encapsulated in its inner layer, and the angiogenic ability of FGF-1 delivered from the external layer in an omentum pouch. Following isolation and culture, islets were encapsulated in the inner core of microspheres ($500-600{\mu}m$ in diameter) with a semi-permeable poly-L-ornithine (PLO) membrane separating two alginate layers, and both unencapsulated and encapsulated islet function was assessed by a dynamic glucose perifusion. For angiogenesis experiments, one group of microcapsules without FGF-1 (control) and another (test) containing FGF-1 with heparin encapsulated in the external layer were made. One hundred microcapsules of each group were transplanted in Lewis rats (n = 5/group) and were retrieved after 14 days for assessment of angiogenesis. Glucose perifusion of unencapsulated and encapsulated islets resulted in similar stimulation indices. The release of FGF-1 resulted in increased vascular density compared to controls. In conclusion, islets encapsulated in the core of multilayer alginate microcapsules maintain functionality and the microcapsule's external layer is effective in delivery of FGF-1 to enhance graft neovascularization in a retrievable omentum pouch.

키워드

참고문헌

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