Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Inhibitory Effects of Syzygium aromaticum Ethanol Extracts on IgE Mediated RBL-2H3 cell Activation

IgE 매개 RBL-2H3 세포 활성화에 대한 정향 에탄올 추출물의 억제 효과

  • Chung, Joon-Hee (Department of Meridian & Acupoint, College of Oriental Medicine, Semyung University) ;
  • Kim, Yong-Min (Clinical Trial Center for Bio-Industry at Semyung University) ;
  • Park, Jong-Phil (Clinical Trial Center for Bio-Industry at Semyung University) ;
  • Kim, Tae-Yeon (Clinical Trial Center for Bio-Industry at Semyung University) ;
  • Kim, Ee-Hwa (Department of Meridian & Acupoint, College of Oriental Medicine, Semyung University)
  • 정준희 (세명대학교 한의과대학 경락경혈학교실) ;
  • 김용민 (세명대학교 한방바이오산업 임상지원센터) ;
  • 박종필 (세명대학교 한방바이오산업 임상지원센터) ;
  • 김태연 (세명대학교 한방바이오산업 임상지원센터) ;
  • 김이화 (세명대학교 한의과대학 경락경혈학교실)
  • Received : 2014.02.14
  • Accepted : 2014.03.13
  • Published : 2014.03.27
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Abstract

Objectives : In this report, we investigated the effect of ethanol extract of Syzygium aromaticum(L.) Merr. & Perry.(SAE) on the RBL-2H3 cell-mediated allergic response and studied its possible mechanisms of action. Methods : Cytotoxicity on RBL-2H3 cell was evaluated by MTT assay. Anti-allergic activity of SAE was assessed by ${\beta}$-Hexosaminidase and Histamine secretion, ${\beta}$- Hexosaminidase and Histamine secretion were measured by ELISA assay. Evaluate the mechanisms of effect of SAE on the secretion of degranulate mediators, we examined the effect of SAE on the activation of mitogen-activated protein kinases using western blot analysis. Results : SAE had no cytotoxicity on rat basophilic leukemia cell(RBL-2H3). Moreover SAE dose-dependently inhibited RBL-2H3 cell degranulation and histamine release. SAE specifically blocked the IgE-induced p38 mitogen-activated protein kinase activation. Conclusions : Our findings provide evidence that Syzygium aromaticum ethanol extract inhibits mast cell derived allergic reaction, and also demonstrate the involvement of p38 MAPK phosphorylation.

목적 : 본 연구에서는 정향 에탄올 추출물이 RBL-2H3 세포 매개 알레르기 반응에 대해 미치는 영향과 그 작용기전에 대해 연구했다. 방법 : 정향 에탄올 추출물의 RBL-2H3 세포에 대한 독성 여부는 MTT 분석을 통해 평가했다. 정향 에탄올 추출물의 항알러지 작용은 효소결합면역 분석방법(ELISA)을 이용해 ${\beta}$-Hexosaminidase과 Histamine의 분비량을 측정하여 평가하였다. 정향 에탄올 추출물의 작용기전에 대해서는 유사 분열물질-활성화단백질인산화효소(mitogen-activated protein kinase, MAPK)를 western blot 법을 이용하여 측정함으로써 평가하였다. 결과 : 정향의 에탄올 추출물은 RBL-2H3 세포에 대해 독성을 나타내지 않는 농도에서 RBL-2H3 세포의 탈과립과 히스타민 분비를 유의하게 억제하였으며, p38 MAPK의 활성을 차단하였다. 결론 : 본 연구의 결과 정향의 에탄올 추출물은 비만세포에서 유래된 알러지 반응을 억제하는 효과가 있으며, 또한 그 작용기전은 p38 MAPK 인산화와 연계되어 있을 것으로 사료된다.

Keywords

References

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