Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Morphological and Genetic Variation of Two Populations of Platichthys stellatus (Pleuronectidae, PISCES) from the East Sea

동해 강도다리(Platichthys stellatus) 2개체군의 형태 및 분자변이

  • Received : 2013.12.11
  • Accepted : 2014.02.10
  • Published : 2014.02.28
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Abstract

Morphological and genetic variation of two populations of Platichthys stellatus were investigated based on 30 individuals each, collected from Uljin (seedling release area) and Pohang (control) in Korea. Morphological analyses demonstrated that the two populations of P. stellatus were well distinguishable in body color of the blind side and fin shape. Mitochondrial DNA control region analysis indicated no significant differences between the two populations ($F_{ST}=-0.00849$, P>0.05). We also analyzed microsatellite DNA loci of the two populations using six markers. Observed heterozygosity ($H_O$) and expected heterozygosity ($H_E$) were 0.550 and 0.592, respectively, in P. stellatus from Uljin, but 0.700 and 0.737 in P. stellatus from Pohang. An index of differentiation in genetic structure revealed significant differences between the two populations ($F_{ST}=0.0208$, P<0.05). Our results suggest that the Uljin population may be comprised of released P. stellatus, whereas the Pohang population may be wild P. stellatus, highlighting the necessity of continuous monitoring of the two populations.

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References

  1. Ball AO, Beal MG, Chapman RW and Sedberry GR. 2007. Population structure of red porgy, Pagrus pagrus, in the Atlantic Ocean. Mar Biol 150, 1321-1332. https://doi.org/10.1007/s00227-006-0425-y
  2. Bell J, Leber K, Blankenship HL, Loneragan N and Masuda R. 2008. A new era for restocking, stock enhancement and sea ranching of coastal fisheries resource. Rev Fish Sci 16, 1-9.
  3. Byun SG, Jeong MH, Lee JH, Lee BI, Ku HD, Park SU, Kim YC, Chang YJ. 2008. Diel rhythm of oxygen consumption of the starry flounder Platichthys stellatus by water temperature. J Kor Fish Soc 41, 113-118. https://doi.org/10.5657/kfas.2008.41.2.113
  4. Byun SG, Lee BI, Lee JH, Ku HD, Park SU, Yun SM, Hwang SY, Kim YC and Han HG. 2007. Egg development and morphological change of larvae and juveniles of the Starry Flounder, Platichthys stellatus. Kor J Ichthyol 19, 350-359.
  5. Clement M, Posaka D and Crandall KA. 2000. TCS: a computer program to estimate gene genealogies. Mol Ecol 9, 1657-1659. https://doi.org/10.1046/j.1365-294x.2000.01020.x
  6. Excoffier L, Laval G and Schneider S. 2005. Arlequin ver. 3.0:an integrated software package for population genetics data analysis. Evol Bioinform 1, 47-50.
  7. Fausto T, Andrea C, Maria V, Corrado P, Anna MS. 1999. Comparative analysis of a mitochondrial DNA control region fragment amplified from three adriatic flatfish species and molecular phylogenesis of Pleuronectiformes. Mar Biotechnol 1, 20-24. https://doi.org/10.1007/PL00011747
  8. Gonzalez BE, Umino T, Kazuya N. 2008. Stock enhancement program for black sea bream (Acanthopagrus schlegelii) in Hiroshima Bay: Monitoring the genetic effects. Aquaculture 276, 36-43. https://doi.org/10.1016/j.aquaculture.2008.02.004
  9. Goudet J. 1995. FSTAT (version 1.2): a computer program to calculate F-statistics. J Heredity 86, 485-486. https://doi.org/10.1093/oxfordjournals.jhered.a111627
  10. Hamasaki K, Kitada S, Toriya S, Shishidou H, Sugaya T. 2010. Genetic effects of hatchery fish on wild population in red sea bream Pagrus major (Perciformes, Sparidae) inferred from a partial sequence of mitochondrial DNA. J Fish Biol 77, 2123-2136. https://doi.org/10.1111/j.1095-8649.2010.02826.x
  11. Hubbs CL. and Lagler KF. 2004. Fishes of the Great Lakes Region. Revised ed. Michigan University Press, Ann Arbor, 332.
  12. Jeong DS, Jeon CY. 2008. Genetic variability and population structure of olive flounder Paralichthys olivaceus from stocked areas using microsatellite DNA markers. Kor J Ichthyol 20, 156-162.
  13. Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111-120. https://doi.org/10.1007/BF01731581
  14. Kitada S and Kishino H. 2006. Lessons learned from Japanese marine finfish stock enhancement programmes. Fish Res 80, 101-112. https://doi.org/10.1016/j.fishres.2006.03.019
  15. Kitada S, Shishidou H, Sugaya T, Kitakado T, Hamasaki K. and Kishino H. 2009. Genetic effects of long-term stock enhancement programs. Acuaculture 290, 69-79. https://doi.org/10.1016/j.aquaculture.2009.02.011
  16. Li Q, Park C, Endo T and Kijima A. 2004. Loss of genetic variation at microsatellite loci in hatchery strains of the Pacific abalone (Haliotis discus hannai). Aquaculture 235, 207-222. https://doi.org/10.1016/j.aquaculture.2003.12.018
  17. Lim HK and Kim SY. 2007. Effect of exogenous hormones on spermiation in the starry flounder Platichthys stellatus. J Kor Fish Soc 40, 374-379. https://doi.org/10.5657/kfas.2007.40.6.374
  18. Lindsey CC. 1988. Factors controlling variation in Fish Physiology, Ed. by Hoar WS and Randall DJ. Academic Press, New York, U.S.A., 197-274.
  19. Liu Y, Chen S, Li B. 2005. Assessing the genetic structure of three Japanese flounder (Paralichthys olivaceus) stocks by microsatellite makers. Aquaculture 243, 103-111 . https://doi.org/10.1016/j.aquaculture.2004.10.024
  20. Matsumiya Y, Kanamaru H, Oka M and Tateishi M. 1987. Morphometric comparison and differntiation between artificially-released and wild red sea bream (Pagrus major). J Appl Ichthyol 3, 49-55. https://doi.org/10.1111/j.1439-0426.1987.tb00452.x
  21. Miao G, Shao C, Ma H, Liao X and Chen S. 2009. Isolation and characterization of polymorphic microsatellite loci from a dinucleotide-enriched genomic library of starry flounder (Platichthys stellatus) and cross-species amplification. Conserv Genet 10, 631-633. https://doi.org/10.1007/s10592-008-9594-1
  22. Norris AT, Bradley DG and Cunnungham EP. 2000. Parentage and relatedness determination in farmed Atlantic salmon (Salmo salar) using microsatellite markers, Aquaculture 182, 73-83. https://doi.org/10.1016/S0044-8486(99)00247-1
  23. Ortega-Villaizan Romo MM, Aritaki M, Taniguchi N. 2006. Pediree analysis of recaptureed fish in the stock enhancement program of spotted halibut Verasper variegatus. Fish Sci 72, 48-52. https://doi.org/10.1111/j.1444-2906.2006.01115.x
  24. Park JY, Kim KK, Kim MJ. 2010. Genetic differences between wild and cultured populations in olive flounder in Korea based on mitochondrial DNA analysis. J Life Sci 20, 614-617. https://doi.org/10.5352/JLS.2010.20.4.614
  25. Parrish BB and Saville A. 1965. The biology of the north-east atlantic herring population. Ocean Mar Biol Ann Rev 3, 323-372.
  26. Raymond M, Rousset F. 1995. GENEPOP (version 1.2): populationgenetics software for exact tests and ecumenicism. J Heredity 86, 248-249.
  27. Saito T, Washio S, Dairiki K, Shimojo M, Ito S and Sugita H. 2008. High gene flow in Girella punctata (Perciformes, Kyphosidae) among the Japanese Islands inferred from partial sequence of the control region in mitochondrial DNA. J Fish Biol 73, 1937-1945. https://doi.org/10.1111/j.1095-8649.2008.01997.x
  28. Sekino M, Hara M and Taniguchi N. 2002. Loss of microsatellite and mitochondrial DNA variation in hatchery strains of Japanese flounder Paralichthys olivaceus. Aquaculture 213, 101-122. https://doi.org/10.1016/S0044-8486(01)00885-7
  29. Slatkin M and Hudson RR. 1991. Pairwise conparisons of mitochondrial DNA sequences in stable and exponentially growing populations montgomery. Genet 129, 555-562.
  30. Sobajima N, Munekiyo M and Funata H. 1986. Possibility of differentiation between the artificially released and the wild red sea bream by means of lack of the inter-nostril epidermis. Bulletin of Kyoto Institute of Oceanic and Fishery Science 10, 35-40.
  31. Thompson JD, Higgins DG and Gibson TJ. 1994 CLUSTAL W:improving the sensitivity of progressive multiple sequence aligment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl Acid Res 22, 4673-4680. https://doi.org/10.1093/nar/22.22.4673
  32. Wang M, Zhang X, Yang T, Han Z, Yanagimoto T and Gao T. 2008. Genetic diversity in the mtDNA control region and population structure in the Sardinella zunasi Bleeker. African J Biotech 24, 4384-4392.
  33. Weir BS and Cockrham CC. 1984. Estimating F-statstics for the analysis of population structure. Evol 38, 1358-1370. https://doi.org/10.2307/2408641