Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Identification and Characterization of Entomopathogenic Fungi Isolated from Bemisia tabaci in Korea

담배가루이(Bemisia tabaci )에서 분리한 곤충병원성진균의 동정 및 병원성 검정

  • Park, Hyun-Rho (Gyeongbuk Agricultural Research & Extension Services) ;
  • Ryu, Young-Hyun (Gyeongbuk Agricultural Research & Extension Services) ;
  • Yeon, Il-Kyen (Gyeongbuk Agricultural Research & Extension Services) ;
  • Nam, Sung-Hee (Applied Entomology Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Dong-Geun (Gyeongbuk Agricultural Research & Extension Services) ;
  • Han, Myung-Sae (Department of Natural Fiber Science, Kyungpook National University Daegu)
  • 박현로 (경상북도농업기술원) ;
  • 류영현 (경상북도농업기술원) ;
  • 연일권 (경상북도농업기술원) ;
  • 남성희 (농촌진흥청 국립농업과학원 농업생물부 곤충산업과) ;
  • 김동근 (경상북도농업기술원) ;
  • 한명세 (경북대학교 농업생명대학 천연섬유학과)
  • Received : 2013.12.04
  • Accepted : 2013.10.07
  • Published : 2014.03.01
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Abstract

Entomopathogenic fungi were isolated from Bemisia tabaci in an Oriental melon field, and their growth characteristics, factors related to a natural outbreak, and infectivity against Bemisia tabaci, Tetranychus urticae, and Frankliniella intonsa were investigated. The isolates had erect conidiophores bearing whorls of 4-6 phialides with a swollen base where cylindrical conidia of $3.0-3.4{\mu}m$ were attached. The isolates were identified as Isaria fumosorosea on the basis of morphological characteristics and an ITS sequence with 99% similarity. I. fumosorosea IFs-08 grew well on Sabouraud dextrose agar+yeast extract medium(3.2 mm/day/$24^{\circ}C$); it grew better at $35^{\circ}C$ than at $15^{\circ}C$. The isolates of I. fumosorosea-IFs were highly infective and killed 93.9-96.7% B. tabaci, 84.9-92.0% T. urticae, and 81.5-84.4% F. intonsa in bioassay, whereas three isolates (Isaria tenuipes, Isaria farinosa, and Isaria fumosorosea) from KACC showed a low infectivity of 10-20%. To the best of our knowledge, this is the first report of I. fumosorosea isolated from B. tabaci in Korea.

자연 감염된 담배가루이로부터 곤충병원성진균을 분리 동정하고, 이 균주에 대하여 배양특성, 자연발병 요인, 그리고 주요 온실해충인 담배가루이, 점박이응애, 대만총채벌레에 대한 살충력을 검정하였다. 분리 균주의 형태적 특성은 직립한 분생자병에 윤생으로 호리병 형태의 4-6개 phialide를 지녔고, 분생포자의 크기는 $3.0-3.4{\mu}m$ 이었다. 분리 균주의 rDNA의 ITS영역 염기서열 분석에서 Isaria fumosorosea (AF461747)와는 99%의 높은 염기서열 유사도를 보였다. Potato dextrose agar, Sabouraud maltose agar+yeast extract 그리고 Sabouraud dextrose agar+yeast extract 배지에서 IFs 균주는 $24^{\circ}C$의 SDAY 배양에서 균사 성장이 가장 양호하였으며, $15^{\circ}C$보다는 $35^{\circ}C$의 고온에서 균사 성장이 좋았다. I. fumosorosea IFs-08, -09 균주는 담배가루이에 대하여 각각 96.7, 93.9%의 높은 살충율을 보였으며, 점박이응애(92.0, 84.9%) 그리고 대만총채벌레(84.4, 81.5%)에서도 80-90%정도의 살충율을 보인 반면 KACC로부터 분양받은 Isaria tenuipes, Isaria farinosa, Isaria fumosorosea 균주들은 10-20% 내외의 낮은 살충율을 나타내었다.

Keywords

References

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