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해양배양기 내 중탄산염 공급에 따른 Tetraselmis sp. KCTC12432BP 증식에 관한 연구

Investigation of Microalgal Growth, Tetraselmis sp. KCTC12432BP by Supplying Bicarbonate on the Ocean Cultivation

  • Cho, Yonghee (Department of Biological Engineering, Inha University) ;
  • Shin, Dong-Woo (Department of Biological Engineering, Inha University) ;
  • Lee, Sangmin (Department of Biological Engineering, Inha University) ;
  • Jeon, Hyonam (Department of Biological Engineering, Inha University) ;
  • Ryu, Young-Jin (Department of Biological Engineering, Inha University) ;
  • Lee, Jong-Chan (Department of Biological Engineering, Inha University) ;
  • Lim, Sang-Min (Department of Biological Engineering, Inha University) ;
  • Lee, Choul-Gyun (Department of Biological Engineering, Inha University)
  • 투고 : 2014.12.30
  • 심사 : 2015.01.14
  • 발행 : 2014.12.31

초록

The ocean provide great benefits for microalgal mass cultures with maintaining stable temperature due to high specific heat, mixing by wave energy, and providing large area for large-scale microalgae cultures. In this study, we cultivated a marine green microalga, Tetraselmis sp. KCTC12432BP, using marine photobioreactors on the ocean for investigating the effect of $NaHCO_3$ concentration on the biomass productivities and evaluating the potential of ocean microalgae culture. The culture medium consist of three fold concentrated f/2-Si with 4 g/L of $NaHCO_3$, which is dissolved in natural seawater. After 11 days of cultivation, the cultures reached stationary phase at biomass concentration of 1.6 g/L. At that time, $NaHCO_3$ concentration of 0, 2, and 4 g/L were fed to the cultures. The daily productivities of 0.11, 0.19, 0.30 g/L/day were attained with feeding rate of 0, 2, and 4 g/L $NaHCO_3$, respectively. Biomass productivity of Tetraselmis sp. KCTC12432BP was a function of the $NaHCO_3$ feeding rate as expected. This research shows that the microalgae can grow with $NaHCO_3$ as carbon source in marine photobioreactors on the ocean while exploiting various benefits of ocean cultivation.

키워드

참고문헌

  1. Al-Qasmi, M., Raut, N., Talebi, S., Al-Rajhi, S., and Al-Barwani, T. 2012. A review of effect of light on microalgae growth. Paper presented at the Proceedings of the world congress on engineering.
  2. Badger, M.R., D. Hanson, and G.D. Price. 2002. Evolution and diversity of $CO_2$ concentrating mechanisms in cyanobacteria. Functional Plant Biology, 29(2-3), 161-173. https://doi.org/10.1071/PP01213
  3. Badger, M.R. and G.D. Price. 2003. $CO_2$ concentrating mechanisms in cyanobacteria: Molecular components, their diversity and evolution. Journal of Experimental Botany. 54(383), 609-622. https://doi.org/10.1093/jxb/erg076
  4. Camacho, F., Molina, E., Martinez, M. E., Sanchez, S., and Garcia, F. 1990. Continuous culture of the marine microalga Tetraselmis sp. productivity analysis. Aquaculture. 90(1), 75-84. https://doi.org/10.1016/0044-8486(90)90284-T
  5. Chisti, Y. 2008. Biodiesel from microalgae beats bioethanol. Trends in Biotechnology. 26(3), 126-131. https://doi.org/10.1016/j.tibtech.2007.12.002
  6. da Cruz Coelho, A. A., Barros, M. U. G., Bezerra, J. H. C., da Silva, J. W. A., Moreira, R. L., and Farias, W. R. L. 2012. Growth of the microalgae Tetraselmis tetrathele and nitrate depletion in culture medium Guillard f/2 and Conway. Biological Sciences. 35(2), 163-168.
  7. Ghasemi, Y., Rasoul-Amini, S., Naseri, A. T., Montazeri-Najafabady, N., Mobasher, M. A. and Dabbagh, F. 2012. Microalgae biofuel potentials. Applied Biochemistry and Microbiology. 48, 126-144. https://doi.org/10.1134/S0003683812020068
  8. Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewit z, M., Seibert, M. and Darzins, A. 2008. Microalgal triac ylglycerols as feedstocks for biofuel production: perspec tives and advances. The Plant Journal, 54, 621-639 https://doi.org/10.1111/j.1365-313X.2008.03492.x
  9. Koh, L. P., and Ghazoul, J. 2008. Biofuels, biodiversity, and people: understanding the conflicts and finding opp ortunities. Biological conservation, 141(10), 2450-2460. https://doi.org/10.1016/j.biocon.2008.08.005
  10. Lim, D. K., Garg, S., Timmins, M., Zhang, E. S., Thomas-Hall, S. R., Schuhmann, H., and Schenk, P. M. 2012. Isolation and evaluation of oil-producing microalgae from subtropical coastal and brackish waters. PLoS One, 7(7), e40751. https://doi.org/10.1371/journal.pone.0040751
  11. Mata, T. M., Martins, A. A., and Caetano, N. S. 2010. Microalgae for biodiesel production and other applications: A review. Renewable and Sustainable Energy Reviews, 14(1), 217-232. https://doi.org/10.1016/j.rser.2009.07.020
  12. Tran, H. L., Kwon, J. S., Kim, Z. H., Oh, Y., & Lee, C. G. 2010. Statistical optimization of culture media for growth and lipid production of Botryococcus braunii LB572. Biotechnology and Bioprocess Engineering, 15(2), 277-284. https://doi.org/10.1007/s12257-009-0127-7
  13. Voleti, R. S. 2012. EXPERIMENTAL STUDIES OF VERTICAL MIXING IN AN OPEN CHANNEL RACEWAY FOR ALGAE BIOFUEL PRODUCTION. Master of Science, Utah State University, Logan, Utah.
  14. Way, C. 2012. Standard methods for the examination of water and wastewater.
  15. White, D., Pagarette, A., Rooks, P., and Ali, S. 2013. The effect of sodium bicarbonate supplementation on growth and biochemical composition of marine microal gae cultures. Journal of Applied Phycology, 25(1), 153-165. https://doi.org/10.1007/s10811-012-9849-6
  16. Xiaodong, D., Y. L. and Xiaowen F. 2009. Microalgae: A promising feedstock for biodiesel. African Journal of Microbiology Research. 3(13), 1008-1014

피인용 문헌

  1. Theoretical Calculations on the Feasibility of Microalgal Biofuels: Utilization of Marine Resources Could Help Realizing the Potential of Microalgae vol.11, pp.11, 2016, https://doi.org/10.1002/biot.201600041
  2. Phycospheric Native Bacteria Pelagibaca bermudensis and Stappia sp. Ameliorate Biomass Productivity of Tetraselmis striata (KCTC1432BP) in Co-cultivation System through Mutualistic Interaction vol.8, pp.1664-462X, 2017, https://doi.org/10.3389/fpls.2017.00289
  3. Development of a qPCR assay for tracking the ecological niches of genetic sub-populations within Pseudo-nitzschia pungens (Bacillariophyceae) vol.63, pp.None, 2014, https://doi.org/10.1016/j.hal.2016.12.002
  4. 중력 여과장치 이용 미세조류 수확을 위한 여과막의 효율성 비교 vol.9, pp.1, 2014, https://doi.org/10.15433/ksmb.2017.9.1.008