Asian Pacific Journal of Cancer Prevention

  • 제15권22호
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  • Pages.9945-9948
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  • 2014
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Tim-3 Expression by Peripheral Natural Killer Cells and Natural Killer T Cells Increases in Patients with Lung Cancer - Reduction after Surgical Resection

  • Xu, Li-Yun (Cell and Molecular Biology Laboratory, Zhoushan Hospital) ;
  • Chen, Dong-Dong (Cell and Molecular Biology Laboratory, Zhoushan Hospital) ;
  • He, Jian-Ying (Cell and Molecular Biology Laboratory, Zhoushan Hospital) ;
  • Lu, Chang-Chang (Lung Cancer Research Center of Zhoushan, Zhoushan Hospital) ;
  • Liu, Xiao-Guang (Cell and Molecular Biology Laboratory, Zhoushan Hospital) ;
  • Le, Han-Bo (Department of Cardio-Thoracic Surgery, Zhoushan Hospital) ;
  • Wang, Chao-Ye (Department of Cardio-Thoracic Surgery, Zhoushan Hospital) ;
  • Zhang, Yong-Kui (Lung Cancer Research Center of Zhoushan, Zhoushan Hospital)
  • 발행 : 2014.12.18
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초록

Background: The purpose of this study was to investigate Tim-3 expression on peripheral CD3-CD56+ natural killer (NK) cells and CD3+CD56+ natural killer T (NKT) cells in lung cancer patients. Materials and Methods: We analyzed Tim-3+CD3-CD56+ cells, Tim-3+CD3-$CD56^{dim}$ cells, Tim-3+CD3-$CD56^{bright}$ cells, and Tim-3+CD3+CD56+ cells in fresh peripheral blood from 79 lung cancer cases preoperatively and 53 healthy controls by flow cytometry. Postoperative blood samples were also analyzed from 21 members of the lung cancer patient cohort. Results: It was showed that expression of Tim-3 was significantly increased on CD3-CD56+ cells, CD3-$CD56^{dim}$ cells and CD3+CD56+ cells in lung cancer patients as compared to healthy controls (p=0.03, p=0.03 and p=0.04, respectively). When analyzing Tim-3 expression with cancer progression, results revealed more elevated Tim-3 expression in CD3-CD56+ cells, CD3-$CD56^{dim}$ cells and CD3+CD56+ cells in cases with advanced stages (III/IV) than those with stage I and II (p=0.02, p=0.04 and p=0.01, respectively). In addition, Tim-3 expression was significantly reduced on after surgical resection of the primary tumor (p<0.01). Conclusions: Tim-3 expression in natural killer cells from fresh peripheral blood may provide a useful indicator of disease progression of lung cancer. Furthermore, it was indicated that Tim-3 might be as a therapeutic target.

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참고문헌

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