The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Characterization of Monilinia fructicola Associated with Brown Rot of Cherry Fruit in Korea

체리 잿빛무늬병에 관여하는 Monilinia fructicola의 특성

  • 최효원 (농촌진흥청 국립농업과학원) ;
  • 홍성기 (농촌진흥청 국립농업과학원) ;
  • 이영기 (농촌진흥청 국립농업과학원) ;
  • 남영주 (농촌진흥청 국립농업과학원) ;
  • 이재금 (농촌진흥청 국립농업과학원) ;
  • 심홍식 (농촌진흥청 국립농업과학원)
  • Received : 2014.10.14
  • Accepted : 2014.12.17
  • Published : 2014.12.30
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Abstract

In August 2013, brown rot was observed on cherry fruits (Prunus avium L.) in Hwaseong city, Korea. Fruit rot first appears as small, circular brown spots that increase rapidly in size causing the entire fruit to rot. Grayish spores appear in tufts on rotted areas. Based on these morphological characteristics, the two isolates were identified as Monilinia fructicola. Molecular analysis of 5.8S subunit and flanking internal transcribed spacers (ITS1 and ITS2) was performed to confirm the identification. The ITS sequences had 100% identity with those of other reference M. fructicola isolates of NCBI GenBank. Pathogenicity was tested using spore suspension inoculation on wounded or unwounded cherry fruits. The lesions were observed on wounded and unwounded fruits after inoculation for 7 days. This is the first report on M. fructicola causing brown rot on cherry fruits in Korea.

2013년 8월, 경기도 화성시에 위치한 과수원에서 체리 과실에 회색의 포자 덩어리가 발생하는 잿빛무늬병 증상이 관찰되었다. 과실에 갈색의 수침상 원형 병반이 나타나고, 진전되면 과실 전체가 부패하였다. 부패된 과실에 회갈색 내지 연회색의 포자덩어리가 관찰되었다. 병든 체리 과실에서 2개의 Monilinia균을 분리하여 균학적 특성을 조사한 결과 M. fructicola로 동정되었다. 이러한 결과를 확인하기 위하여 ITS 영역의 염기서열을 분석하였다. 그 결과, 분리균은 모두 M. fructicola로 확인되었다. 체리 과실을 상처구와 무상처구로 구분하고, 포자현탁액을 접종하여 병원성을 확인한 결과, 접종 7일 후 상처의 유무에 관계없이 분리균을 접종한 부위에서 수침상의 둥근 병반이 관찰되었다. 따라서 본 병을 Monilinia fructicola에 의한 체리잿빛무늬병으로 명명하며, 이 균에 의한 잿빛무늬병의 발생을 국내 최초로 보고한다.

Keywords

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