Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지
DOI QR코드

DOI QR Code

Synergistic Effect of Brazilein in Combination with Hygromycin-b against Staphylococcus aureus

메티실린-내성 포도상구균에 대하여 Brazilein 혼합에 따른 항생제 Hygromycin-b의 상승효과

  • Lee, Young Seob (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Dae Young (Department of Herbal Crop Research, NIHHS, RDA) ;
  • An, Tae Jin (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Jeong Hoon (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Ahn, Young Sup (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Cha, Seon Woo (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Mun, Su Hyun (BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kang, Ok Hwa (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Institute of Biotechnology, Wonkwang University) ;
  • Kwon, Dong Yeul (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Institute of Biotechnology, Wonkwang University) ;
  • Han, Sin Hee (Department of Herbal Crop Research, NIHHS, RDA)
  • 이영섭 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이대영 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 안태진 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이정훈 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 안영섭 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 차선우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 문수현 (원광대학교 한의학전문대학원 BK21사업팀) ;
  • 강옥화 (원광대학교 약학대학 한약학과) ;
  • 권동렬 (원광대학교 약학대학 한약학과) ;
  • 한신희 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • Received : 2014.07.07
  • Accepted : 2014.10.16
  • Published : 2014.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a substantial contributor to morbidity and mortality. In search of a natural products capable of inhibiting this multidrug resistant bacteria, we have investigated the antimicrobial activity of brazilein (BRZ) isolated from Caesalpinia sappan L. (Leguminosae) against 8 different strains of Staphylococcus aureus (S. aureus). New antimicrobial activity was found using the minimum inhibitory concentrations (MICs), broth dilution as well as checkerboard method. Against the 8 strains, the minimum inhibitory concentrations of BRZ were in the range of $62.5-500{\mu}g/mL$. From those results we performed the checkerboard test to determine the synergism of BRZ in combination with Hygromycin-b (HgB) against 4 strains. The combined activity of BRZ and HgB against 4 strains resulted in a fractional inhibitory concentrations index (FICI) ranging from 0.18-0.5. The effect of BRZ with HgB was found to be synergistic. We found that BRZ reduced the MICs of HgB. BRZ and HgB could lead to the development of new combination antibiotics against MRSA infection.

Keywords

References

  1. Aqil F, Khan MS, Owais M and Ahmad I. (2005). Effect of certain bioactive plant extracts on clinical isolates of beta-lactamase producing methicillin resistant Staphylococcus aureus. Journal of Basic Microbiology. 45:106-114. https://doi.org/10.1002/jobm.200410355
  2. Choi JG, Kang OH, Lee YS, Oh YC, Chae HS, Jang HJ, Kim JH, Sohn DH, Shin DW, Park H and Kwon DY. (2008). In vitro activity of methyl gallate isolated from Galla Rhois alone and in combination with ciprofloxacin against clinical isolates of salmonella. Journal of Microbiology and Biotechnology. 18:1848-1852.
  3. Clinical and Laboratory Standards Institute(CLSI). (2012). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved standard(9th Ed.). Clinical and Laboratory Standards Institute. Wayne. PA, USA. CLSI document. M7-A5.
  4. Eum WS, Lee KJ, Kim DW, Lim SS, Kang IJ, Park J and Choi SY. (2013). Anti-Inflammatory effects of extracts from Caesalpinia sappan L. on skin inflammation. Journal of the Korean Society of Food Science and Nutrition. 42:384-388. https://doi.org/10.3746/jkfn.2013.42.3.384
  5. Fu Y, Zu Y, Chen L, Shi X, Wang Z, Sun S and Efferth T. (2007). Antimicrobial activity of clove and rosemary essential oils alone and in combination. Phytotherapy Research. 21:989-994. https://doi.org/10.1002/ptr.2179
  6. Lee JH, Lee SH, Park CG, Park CB, Kim OT, Choi AJ, Kim YJ and Cha SW. (2013). Cultivation characteristics and variation of artemisinin contents by harvest time of Artemisia annua L. distributed in Korea. Korean Journal of Medicinal Crop Science. 21:433-438. https://doi.org/10.7783/KJMCS.2013.21.6.433
  7. Lee JH, Song KY, Hyeon JY, Hwang IG, Kwak HS, Han JA, Chung YH and Seo KH. (2010). Comparison of standard culture method and real-time PCR assay for detection of Staphylococcus aureus in processed and unprocessed foods. Korean Journal for Food Science of Animal Resources. 30:410-418. https://doi.org/10.5851/kosfa.2010.30.3.410
  8. Lee JY, Wang HJ, Shin DB and Cho YS. (2013). Antibiotic resistance and bacterial biofilm formation by Staphylococcus aureus strains isolated from various foods. Korean Journal of Microbiology and Biotechnology. 41:96-104. https://doi.org/10.4014/kjmb.1208.08012
  9. Jang S, Kim TH, Lee AR, Lee AY, Choi G and Kim HK. (2012). Monitoring of heavy metal contents in commercial herbal medicines. Korean Journal of Medicinal Crop Science. 20:434-439. https://doi.org/10.7783/KJMCS.2012.20.6.434
  10. Kalivoda EJ, Horzempa J, Stella NA, Sadaf A, Kowalski RP, Nau GJ and Shanks RMQ. (2011). New vector tools with a hygromycin resistance marker for use with opportunistic pathogens. Molecular Biotechnology. 48:7-14. https://doi.org/10.1007/s12033-010-9342-x
  11. Kang TY, Kang ST, Ihn YH, Lee YH, Cho DY, Lee SJ, Son WG, Heo MS and Jeong DK. (2010). Prevention of salmonella infection in layer hen fed with microbial fermented citrus shell. Journal of Life Science. 2:190-196. https://doi.org/10.5352/JLS.2010.20.2.190
  12. Kettle A and Schacht J. (2002). Antioxidant therapy : A promising approach to the prevention of noise- and drug-induced hearing loss. Korean Journal of Otorhinolaryngology-Head and Neck Surgery. 45:931-935.
  13. Kim DS, Baek NI, Oh SR, Jung KY, Lee IS and Lee HK. (1997). NMR assignment of brazilein. Phytochemistry. 46:177-178. https://doi.org/10.1016/S0031-9422(96)00874-6
  14. Kim E, Choi CH, Kim IH, Lee HI and Song YS. (2013).Antibacterial effect of huanggeumjakyak-tang against methicillinresistant Staphylococcus aureus. Journal of Korean MedicineRehabilitation. 23:15-26.
  15. Kim EA. (2013). Methicillin-resistant Staphylococcus aureus (MRSA) infection in neonates. Neonatal Medicine. 20:354-360. https://doi.org/10.5385/nm.2013.20.3.354
  16. Kim KM, Yoo JH, Choi JH, Park ES, Kim KS, Kim KS, Kim SR, Kim SM, Kim HJ, Jung JS, Yoo KH, Oh HS, Yoon SW, Suh MR, Yoon YK, Lee JY, Jang YS, Jin HY, Kim SW, Kim YR, Kim YS, Kim YS, Kim JU, Kim JM, Peck KR, Lee H, Oh MD, Oh SH, Lee WK, Lee SH, Chung MH, Jung SI, Cheong HJ and Shin WS. (2006). The nationwide surveillance results of nosocomial infections along with antimicrobial resistance in intensive care units of sixteen university hospitals in Korea, 2004. Korean Journal of Nosocomial Infection Control. 11:79-86.
  17. Kim NY, Jang MK, Jeon MJ, Lee DG, Jang HJ, Lee SW, Kim M, Kim SG and Lee SH. (2010). Verification of antimicrobial activities of various pine needle extracts against antibiotic resistant strains of Staphylococcus aureus. Journal of Life Science. 20:589-596. https://doi.org/10.5352/JLS.2010.20.4.589
  18. Kim SJ. (2011). Comparison between antimicrobial susceptibility test and mecA PCR method for reading of methicillin-resistant Staphylococcus aureus. The Korean Journal of Microbiology. 47:381-385.
  19. Kim SK, Lee YS, Lee TJ, Lee TY and Kim HS. (1993). Identification and antimicrobial susceptibility aspects of pathogenic Staphylococcus aureus. The Journal of the Korean Society for Microbiology. 28:251-259.
  20. Kwon HJ, Jung UH, Park HR, Shin DH and Jo SK. (2007). Effects of gamma irradiation on color changes and antioxidative activities of Caesalpinia sappan L. Journal of the Korean Society of Food Science and Nutrition. 36:1055-1061. https://doi.org/10.3746/jkfn.2007.36.8.1055
  21. Leclercq R, Derlot E, Duval J and Courvalin P. (1998). Plasmid mediated resistance to vancomyhcin and teicoplanin. New England Journal of Medicine. 319:157-161.
  22. Pfister P, Risch M, Brodersen DE and Bottger EC. (2003). Role of 16S rRNA helix 44 in ribosomal resistance to hygromycin B. Antimicrobial Agents and Chemotherapy. 47:1496-1502. https://doi.org/10.1128/AAC.47.5.1496-1502.2003
  23. Shin SH and Seong IW. (2006). Antimicrobial activity or the extracts from Paeonia japonica against methicillin-resistant Staphylococcus aureus. The Korean Journal of Microbiology. 42:54-58.

Cited by

  1. Monitoring of Biological Hazards in Herbal Crops from Korean Market vol.24, pp.2, 2016, https://doi.org/10.7783/KJMCS.2016.24.2.143