Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Anti-inflammatory Activity of Solvent Fractions from Ginseng Berry Extract in LPS-Induced RAW264.7 Cells

인삼열매추출물의 용매분획물이 LPS로 유도된 RAW264.7 세포에 대한 항염활성

  • Lee, Ka Soon (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES) ;
  • Kim, Gwan Hou (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES) ;
  • Seong, Bong Jae (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES) ;
  • Kim, Sun Ick (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES) ;
  • Han, Seung Ho (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES) ;
  • Lee, Sox Su (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES) ;
  • Yang, Hui (Department of Microbiology, College of Medicine, Konyang University) ;
  • Yoo, Yung Choon (Department of Microbiology, College of Medicine, Konyang University)
  • 이가순 (충남농업기술원 금산인삼약초시험장) ;
  • 김관후 (충남농업기술원 금산인삼약초시험장) ;
  • 성봉재 (충남농업기술원 금산인삼약초시험장) ;
  • 김선익 (충남농업기술원 금산인삼약초시험장) ;
  • 한승호 (충남농업기술원 금산인삼약초시험장) ;
  • 이석수 (충남농업기술원 금산인삼약초시험장) ;
  • 양혜 (건양대학교 의과대학) ;
  • 유영춘 (건양대학교 의과대학)
  • Received : 2014.08.27
  • Accepted : 2014.10.13
  • Published : 2014.12.30
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Abstract

Anti-inflammatory activity of the extracts of ginseng berry (GBE) was investigated through the evaluation of its inhibitory effect on the production of inflammatory meditator, nitric oxide(NO), tumor necrocis factor-alpha (TNF-${\alpha}$), interleukin-6 (IL-6) in LPS-induced RAW264.7 macrophage cells. GBE was fractionated using n-hexane, chloroform, ethylacetate, buthanol and $H_2O$, sequentially. RAW264.7 cells were induced $100ng/m{\ell}$ of lipopolysaccharide (LPS) and treated with 0, 1.6, 8, 40 and $200{\mu}g/m{\ell}$ of GBE fractions. LPS-induced NO production on all of GBE fractions was inhibited with increasing added concentration of GBE fractions. Chloroform fraction of GBE was the most effective in inhibiting LPS-induced TNF-${\alpha}$ production. Hexane, chloroform and $H_2O$ fractions of GBE exhibit strong inhibition LPS-induced IL-6 production. Especially, $H_2O$ fractions of GBE was the most effective in inhibiting LPD-induced IL-6 production without significant cytotoxicity in RAW264.7 cells, and reduced the activation of mitogen-activated protein kinases (MAPK) and IkB phosphorylation. These results indicate that $H_2O$ fractions of GBE exhibits strong anti-inflammatory effects by inhibition of NF-kB by inhibition of p-38 on MAPK and IkB phosphorylation.

Keywords

References

  1. Aelberhard EE, Henderson SA, Arabolos NS, Griscavage JM, Castro FE, Barrett CT and Ignarro LJ. (1995). Nonsteroidal anti-inflammatory drugs inhibit expression of the inducible nitric oxide synthase gene. Biochemical and Biophysical Research Communications. 208:1053-1059. https://doi.org/10.1006/bbrc.1995.1441
  2. Attele AS, Zhou YP, Xie JT, Wu JA, Zhang L, Dey L, Pugh W, Rue PA, Polonsky KS and Yuan CS. (2002). Antidiabetic effects of Panax ginseng berry extract and the identification of an effective component. Diabetes. 51:1851-1858. https://doi.org/10.2337/diabetes.51.6.1851
  3. Bredt DS and Snyder SH. (1994). Nitric oxide: A Physiologic messenger molecule. Annual Review of Biochemistry. 63:175-195. https://doi.org/10.1146/annurev.bi.63.070194.001135
  4. Byun MY. (2012). Anti-inflammatory activity of austroinulin from Stevia rebaudiana in LPS-induced RAW264.7 cells. Journal of the Korean Society of Food Science and Nutrition. 41:456-461. https://doi.org/10.3746/jkfn.2012.41.4.456
  5. Cha JY, Kim HJ, Chung CH and Cho YS. (1999). Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. Journal of the Korean Society of Food Science and Nutrition. 28:1310-1315.
  6. Choi JE, Li X, Han YH, Lee KT. (2009). Changes of saponin contents of leaves, stems and flower-buds of Panax ginseng C. A. Meyer by harvesting days. Korean Journal of Medicinal Crop Science. 17:251-256.
  7. Choi SJ, Cho EA, Cho EH, Jeong YJ, Ku CS, Ha BJ and Chae HJ. (2011). Screening of functional materials from solvent fractions of apple flower leaf extract. Journal of Korean Society for Biotechnology and Bioengineering. 26:165-171. https://doi.org/10.7841/ksbbj.2011.26.2.165
  8. Guzik TJ, Korbut R and Adamek-Guzik T. (2003). Nitric oxide and superoxide in inflammation and immune regulation. Journal of Physiological Phamacology. 54:469-487.
  9. Hawiger J. (2001) Innate immunity and inflammation: A transcriptional paradigim. Immunological Research. 23:99-109. https://doi.org/10.1385/IR:23:2-3:099
  10. Higuchi M, Hisgahi N, Taki H and Osawa T. (1990). Cytolytic mechanisms of activated macrophages. tumor necrosis factor and L-arginine-dependent mechanisms act synergistically as the major cytolytic mechanisms of activated macrophages. Journal of Immunology. 144:1425-1431.
  11. Jeon JM, Choi SK, Kim YJ, Jang SJ, Cheon JW and Lee HS. (2011). Antioxidant and antiaging effect of ginseng berry extract fermented by lactic acid bacteria. Journal of the Society of Cosmetic Scientists of Korea. 37:75-81.
  12. Jo NH, Park CY and Lee SJ. (2014). Health food for improving atopic dermatitis and xerosis containing ginseng fruit extracts. Korea. Patent. 10-1382115.
  13. Jung HK, Kang BM, Jang JH, Ahn BK, Yeo JH, Jung WS, Cho JH, Kuk YI, Hyun KH and Cho HW. (2014). Inhibitory effect of Alopecurus aequalis Sobol ethanol extracts on LPSinduced inflammatory response in RAW264.7 cells. Korean Journal of Medicinal Crop Science. 22:98-104. https://doi.org/10.7783/KJMCS.2014.22.2.98
  14. Kim HJ, Yoo KM, Lee S, Kim KT and Hwang IK. (2013a). Physicochemical characteristics of various ginseng seeds. Korean Journal of Food Science Technology. 45:274-278. https://doi.org/10.9721/KJFST.2013.45.3.274
  15. Kim JE, Lee S, Yoo KM, Lee KH, Kim KT, Lee MH and Hwang IK. (2014a). Quality characteristics of ginseng seed oil obtained by different extraction methods. Journal of the Korean Society of Food Science and Nutrition. 43:439-445. https://doi.org/10.3746/jkfn.2014.43.3.439
  16. Kim JK, Kim BS, Park CW, Seo DB, Yoo HR and Lee SJ. (2010). Effect of ginseng-berry extract on the improvement of blood microcirculation and skin brightness. Korean Journal of Oriental Physiology and Pathology. 24:85-90.
  17. Kim JY, Kim SY, Kwon HM, Kim CH, Lee SJ, Park SC and Kim KH. (2014b). Comparision of antioxidant and antiinflammatory activity on chestnut, chestnut shell and leaves of Castanea crenata extracts. Korean Journal of Medicinal Crop Science. 22:8-16. https://doi.org/10.7783/KJMCS.2014.22.1.8
  18. Kim YO, Lee SW, Sohn SH, Kim SY, Oh MS and Kim SK. (2012). Anti-inflammatory effects of water extract of Eucommia ulmoides oliver on the LPS-induced RAW264.7 cells. Korean Journal of Medicinal Crop Science. 20:381-386. https://doi.org/10.7783/KJMCS.2012.20.5.381
  19. Kim ST, Kim HJ, Jang SK, Lee DI and Joo SS. (2013b). Establishment of optimal fermentation conditions for steamdried ginseng berry via friendly bacteria and its antioxidant activity. Korean Journal of Food Science Technology. 45:77-83. https://doi.org/10.9721/KJFST.2013.45.1.77
  20. Kwon HS, Shin HK, Kwon SO, Yeo KM, Kim SM, Kim BN and Kim JK. (2009). Antiinflammatory effects of aqueous extract from red pepper on lipopolysaccharide induced inflammatory responses in murine macrophages. Journal of the Korean Society of Food Science and Nutrition. 38:1289-1294. https://doi.org/10.3746/jkfn.2009.38.10.1289
  21. Lee HN, Kim JK, Kwon GT, Shim JH, Kim JD and Yoon JH. (2012). Anti-inflammatory effects of ethanol extract from bark of Acer barbinerve Maxim. Journal of the Korean Society of Food Science and Nutrition. 41:1242-1247. https://doi.org/10.3746/jkfn.2012.41.9.1242
  22. Lu Y and Foo LY. (2000). Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chemistry. 68:81-85. https://doi.org/10.1016/S0308-8146(99)00167-3
  23. Nam NH. (2006). Naturally occurring NF-$\kappa{B}$ inhibitors. Mini Reviews in Medicinal Chemistry. 6:945-951. https://doi.org/10.2174/138955706777934937
  24. Nam KY. (1996). The new Korean ginseng(constituent and its pharmacological efficacy). Korea Ginseng & Tabacco Research Institute. Daejeon, Korea. p.1-10.
  25. Park CW and Yeom MH. (2014). Cosmetic composition for antiinflammation containing ginseng fruit extracts. Korea. Patent. 10-1427574.
  26. Park EY and Yang KS. (2008). In hibition of nitric oxide production by the extracts of Hibiscus manihot. Yakhak Hoeji. 52:259-263.
  27. Pyo MK, Lee KH, Seol SH, Yoo JH, Hong SC, Kim BR, Lee HJ and Park JD. (2013) Preparation for the extract of ginseng berry containing increased ginsenoside Re. Korea. Patent. 10-1330935.
  28. Seong IH. (2012). Beverage contained de-alcohol activity substance comprising extract of ginseng fruit, ginseng flower stalks, raisin tree fruit and manufacturing method thereof. Korea. Patent. 10-1125130.
  29. Tsao R. (2010). Chemistry and biochemistry of dietary polyphenols. Nutrients. 2:1231-1246. https://doi.org/10.3390/nu2121231
  30. Tizard IR. (1986) Immunology: an introduction inflammation(2nd ed). Saunder College Publishing. New York. NY, USA. p.423-441.
  31. Willeaume V, Kruys V, Mijatovic T and Huez G. (1996). Tumor necrosis factor-alpha production induced by viruses and by lipopolysaccharide in macrophage: Similarities and differences. Journal of Inflammatory. 46:1-12.
  32. Xie JT, Mehendale S and Yuan CS. (2005). Ginseng and diabetes. The Americn Journal of Chiense Medicine. 33:397-404. https://doi.org/10.1142/S0192415X05003004
  33. Yang SA, Pyo BS, Kim SM and Lee KI. (2012). Antibacterial activity and nitric oxide production inhibitory activity of the extract and its fractions from the leaves of Prunus sargentii. Korean Journal of Medicinal Crop Science. 20:308-314. https://doi.org/10.7783/KJMCS.2012.20.4.308
  34. Yeom MH, Lee JY, Kim JS, Park CW, Kim DH and Kim HK. (2010). The anti-aging effects of korean ginseng berry in the skin. Korean Journal of Pharmacognosy. 41:26-30.