Comparison of Volatile Compounds Identified in Different Parts of Peucedanum japonicum Thunberg by Harvest Time

방풍나물의 수확시기에 따른 부위별 향기성분 비교 분석

Headspace volatile compounds of leaf, stem, and root of Peucedanum japonicum Thunberg (PJT) harvested in March and July were isolated, separated, and identified by using a combined system of solid phase microextraction and gas chromatography/mass spectrometry. Identified numbers of volatile compounds were 72, 75, and 63 in March PJT as well as 78, 73, and 69 in July, respectively. Total compounds identified from PJT consisted of alcohols (1~7), aldehydes (7~15), esters (1~4), hydrocarbons (5~9), ketones (1~2), monoterpenes (13~16), monoterpene alcohols (1~5), monoterpene aldehydes (2~4), monoterpene esters (1~4), monoterpene ketones (1~2), sesquiterpenes (16~24), and miscellaneous compounds (2~3). Major volatile compounds of PJT were monoterpenes at all harvest times and in all parts of PJT. The amounts of ${\beta}$-pinene in leaf and stem harvested in March were highest in monoterpenes, followed by ${\alpha}$-pinene and ${\beta}$-myrcene. However, amounts of volatile compounds of monoterpenes in leaf and stem from July PJT were in the decreasing order of ${\beta}$-pinene, ${\beta}$-phellandrene, and ${\beta}$-myrcene. Of the 39 sesquiterpenes identified in PJT, (E)-caryophyllene in March leaf was the most abundant volatile compound, followed by acoradiene in March leaf, ${\beta}$-elemene, and ${\alpha}$-copaene in July stem.

전남 여수시 금오도에서 재배된 방풍나물을 3월과 7월에 수집하여 잎, 줄기, 뿌리로 나누어 휘발성 향기성분을 SPME 법으로 추출하여 GC/MS로 분석하였다. 3월에 수확된 방풍의 잎은 72종, 줄기는 75종, 뿌리는 63종이 동정되었고, 7월에 수확된 방풍의 잎은 78종, 줄기는 73종, 뿌리는 69종이 동정되었다. 이를 종류별로 분류하면 alcohols(1~7종), aldehydes(7~15종), esters(1~4종), hydrocarbons(5~9종), ketones(1~2종), monoterpenes(13~16종), monoterpene alcohols(1~5종), monoterpene aldejydes(2~4종), monoterpene esters(1~4종), monoterpene ketones(1~2종), sesquiterpenes(16~24종), 기타 화합물(2~3종)로 구성되었다. 방풍의 주요 휘발성 향기성분은 다른 화합물에 비해 monoterpene 류가 상대적으로 비율이 높았고 3월의 잎과 줄기는 ${\beta}$-pinene, ${\alpha}$-pinene, ${\beta}$-myrcene 순으로 높게 나타났으며, 뿌리는 ${\beta}$-pinene, ${\beta}$-myrcene, ${\beta}$-phellandrene 순으로 높게 나타났다. 7월의 잎과 줄기는 ${\beta}$-pinene, ${\beta}$-phellandrene, ${\beta}$-myrcene 순으로 높게 나타났고, 7월의 뿌리는 camphene, ${\beta}$-pinene, p-cymene 순으로 나타났다.