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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Assessment of the Changes in the Microbial Community in Alkaline Soils using Biolog Ecoplate and DGGE

Biolog Ecoplate와 DGGE 방법을 이용한 알칼리화 토양의 미생물군집 변화 평가

  • Lee, Eun Young (Department of Environmental and Energy Engineering, The University of Suwon) ;
  • Hong, Sun Hwa (Department of Environmental and Energy Engineering, The University of Suwon)
  • 이은영 (수원대학교 환경에너지공학과) ;
  • 홍선화 (수원대학교 환경에너지공학과)
  • Received : 2013.09.09
  • Accepted : 2013.10.23
  • Published : 2013.10.30
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Abstract

Soil microbial community analysis of farmland soil sprayed with lye in order to use fertilizer in Nigeria was performed. As a control, two kinds of soils not sprayed with lye, located in Eungo and Lagos with general practice in agriculture was selected. Soil sprayed with lye was pH 8.25 through alkalization reaction, while the other soil samples were pH 6.22 and 5.94. Substrate utilization and species diversity index of soil sprayed with lye were low than that of the other soils with the analysis of Biolog ecoplate. As a result of principal component analysis, the relationship between three samples was low. Microbial community analysis was performed by DGGE and most of them were soil uncultured bacterium. Especially, Uncultured Acidobacteria and Uncultured Methylocystis sp., which had been isolated from the rhizosphere of soybean grown in that site were discovered in the soil sprayed with lye.

Keywords

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