Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Relationship between the Methane Production and the CNCPS Carbohydrate Fractions of Rations with Various Concentrate/roughage Ratios Evaluated Using In vitro Incubation Technique

  • Dong, Ruilan (College of Animal Science and Technology, China Agricultural University, State Key Laboratory of Animal Nutrition) ;
  • Zhao, Guangyong (College of Animal Science and Technology, China Agricultural University, State Key Laboratory of Animal Nutrition)
  • Received : 2013.05.05
  • Accepted : 2013.07.02
  • Published : 2013.12.01
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Abstract

The objective of the trial was to study the relationship between the methane ($CH_4$) production and the Cornell Net Carbohydrate and Protein System (CNCPS) carbohydrate fractions of feeds for cattle and the suitability of CNCPS carbohydrate fractions as the dietary variables in modeling the $CH_4$ production in rumen fermentation. Forty-five rations for cattle with the concentrate/roughage ratios of 10:90, 20:80, 30:70, 40:60, and 50:50 were formulated as feed samples. The Menke and Steingass's gas test was used for the measurement of $CH_4$ production. The feed samples were incubated for 48 h and the $CH_4$ production was analyzed using gas chromatography. Statistical analysis indicated that the $CH_4$ production (mL) was closely correlated with the CNCPS carbohydrate fractions (g), i.e. CA (sugars); $CB_1$ (starch and pectin); $CB_2$ (available cell wall) in a multiple linear pattern: $CH_4=(89.16{\pm}14.93)$ $CA+(124.10{\pm}13.90)$ $CB_1+(30.58{\pm}11.72)$ $CB_2+(3.28{\pm}7.19)$, $R^2=0.81$, p<0.0001, n = 45. Validation of the model using 10 rations indicated that the $CH_4$ production of the rations for cattle could accurately be predicted based on the CNCPS carbohydrate fractions. The trial indicated that the CNCPS carbohydrate fractions CA, $CB_1$ and $CB_2$ were suitable dietary variables for predicting the $CH_4$ production in rumen fermentation in vitro.

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References

  1. AOAC. 1990. Official methods of analysis. 15th ed. Arlington (VA): Assoc. Off. Anal. Chem.
  2. Axelsson, J. 1949. The amount of produced methane energy in the European metabolic experiments with adult cattle. Ann. R. Agric. Coll. Sweden 16:404-419.
  3. Blaxter, K. L. and J. L. Clapperton. 1965. Predition of the amount of methane produced by ruminants. Br. J. Nutr. 19:511-522. https://doi.org/10.1079/BJN19650046
  4. Bratzler, J. W. and E. B. Forbes. 1940. The estimation of methane production by cattle. J. Nutr. 19:611-613.
  5. Ellis, J. L., E. Kebreab, N. E. Odongo, B. W. McBride, E. K. Okine, and J. France. 2007. Prediction of methane production from dairy and beef cattle. J. Dairy Sci. 90:3456-3466. https://doi.org/10.3168/jds.2006-675
  6. Goering, H. K. and P. J. Van Soest. 1970. Forage fibre analysis. Agric. Handbook No. 379. ARS-USDA, Washington, DC.
  7. Jentsch, W., M. Schweigel, F. Weissbach, H. Scholze, W. Pitroff, and M. Derno. 2007. Methane production in cattle calculated by the nutrient composition of the diet. Arch. Anim. Nutr. 61: 10-19. https://doi.org/10.1080/17450390601106580
  8. Kriss, M. 1930. Quantitative relations of the dry matter of the food consumed, the heat production, the gaseous outgo, and the insensible loss in body weight of cattle. J. Agric. Res. 40: 283-295.
  9. Liu, J. X., A. Susenbeth, and K.-H. Sudekum. 2002. In vitro gas production measurements to evaluate interactions between untreated and chemically treated rice straws, grass hay, and mulberry leaves. J. Anim. Sci. 80:517-524.
  10. Mills, J. A. N., E. Kebreab, C. M. Yates, L. A. Crompton, S. B. Cammell, M. S. Dhanoa, R. E. Agnew, and J. France. 2003. Alternative approaches to predicting methane emissions from dairy cows. J. Anim. Sci. 81:3141-3150.
  11. Menke, K. H. and H. Steingass. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev. 28:7-55.
  12. Moe, P. W. and H. F. Tyrrell. 1979. Methane production in dairy cows. J. Dairy Sci. 62:1583-1586. https://doi.org/10.3168/jds.S0022-0302(79)83465-7
  13. Offner, A. and D. Sauvant. 2004. Comparative evaluation of the Molly, CNCPS, and LES rumen models. Anim. Feed Sci. Technol. 112:107-130. https://doi.org/10.1016/j.anifeedsci.2003.10.008
  14. Prasad, C. S., C. D. Wood, and K. T. Sampath. 1994. Use of in vitro gas production to evaluate rumen fermentation of untreated and urea-treated finger millet straw (Eleusine coracana) supplemented with different levels of concentrates. J. Sci. Food Agric. 65:457-464. https://doi.org/10.1002/jsfa.2740650414
  15. Shannak, S., K.-H. Sudekum, and A. Susenbeth. 2000. Estimating ruminal crude protein degradation with in situ and chemical fractionation procedures. Anim. Feed Sci. Technol. 85:195-214. https://doi.org/10.1016/S0377-8401(00)00146-2
  16. Sniffen, C. J., J. D. O'Connor, P. J. Van Soest, D. G. Fox, and J. B. Russell. 1992. A net carbohydrate and protein system for evaluating cattle diets: II. carbohydrate and protein availability. J. Anim. Sci. 70:3562-3577.
  17. Seo, S., Sang C. Lee, S. Y. Lee, J. G. Seo, and Jong K. Ha. 2009. Degradation kinetics of carbohydrate fractions of ruminant feeds using automated gas production technique. Asian-Aust. J. Anim. Sci. 22:356-364. https://doi.org/10.5713/ajas.2009.80613
  18. Van Soest, P. J., J. B. Robertson, and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2

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