Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cr(VI) Resistance and Removal by Indigenous Bacteria Isolated from Chromium-Contaminated Soil

  • Long, Dongyan (Institute of Environmental Science and Technology, Zhejiang University) ;
  • Tang, Xianjin (Institute of Environmental Science and Technology, Zhejiang University) ;
  • Cai, Kuan (Global Environmental Technology Co., Ltd.) ;
  • Chen, Guangcun (Institute of Environmental Science and Technology, Zhejiang University) ;
  • Shen, Chaofeng (Institute of Environmental Science and Technology, Zhejiang University) ;
  • Shi, Jiyan (Institute of Environmental Science and Technology, Zhejiang University) ;
  • Chen, Linggui (Institute of Environmental Science and Technology, Zhejiang University) ;
  • Chen, Yingxu (Institute of Environmental Science and Technology, Zhejiang University)
  • Received : 2013.01.03
  • Accepted : 2013.04.03
  • Published : 2013.08.28
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Abstract

The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing ability, with 100 mg/l Cr(VI) being completely removed within 144 h. It could effectively remove Cr(VI) over a wide pH range from 5.5 to 9.5, with the optimal pH of 8.5. The amount of Cr(VI) removed increased with initial Cr(VI) concentration. Data from the time-course analysis of Cr(VI) removal by strain LY6 followed first-order kinetics. Based on the 16S rRNA gene sequence, strain LY6 was identified as Pseudochrobactrum asaccharolyticum, a species that had never been reported for Cr(VI) removal before. Transmission electron microscopy and energy dispersive X-ray spectroscopy analysis further confirmed that strain LY6 could accumulate chromium within the cell while conducting Cr(VI) removal. The results suggested that the indigenous bacterial strain LY6 would be a new candidate for potential application in Cr(VI) pollution bioremediation.

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