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Physicochemical Characteristics of Cheonnyuncho Fruit (Opuntia humifusa) Fermented by Leuconostoc mesenteroides SM

Leuconostoc mesenteroides SM을 이용한 천년초(Opuntia humifusa) 열매 발효물의 물리·화학적 특성

  • Park, Min-Ju (Department of Food Science and Technology, Keimyung University) ;
  • Lee, Sam-Pin (Department of Food Science and Technology, Keimyung University)
  • Received : 2013.03.10
  • Accepted : 2013.06.21
  • Published : 2013.08.31

Abstract

Functional properties of Cheonnyuncho fruit paste (CFP) were enhanced by fermentation, using Leuconostoc mesenteroides SM. The stability of CFP pigment was maintained and microorganisms in crude CFP were sterilized by heating at $80^{\circ}C$ for 30 min. The production of dextran in CFP was increased by increasing the fermentation time and sugar content, resulting in an increase of consistency. The CFP fermented with 20% sucrose contained 4.8% mucilage after fermentation for 2 days. In particular, the production of mucilage was greatly enhanced by fortification with 3% sodium citrate in CFP, resulting in mucilage production of 7%. In addition, the viscous modulus of fermented CFP showed a greater increase than that of the elastic modulus. Thus, it was concluded that CFP fermented by L. mesenteroides SM can be fortified with functional ingredients, such as mucilage and probiotics, with enhanced rheological properties. Therefore fermented CFP can be utilized as the functional ingredients for functional foods.

천년초 열매의 기능성 및 물성을 개선하기 위해서 Leuconostoc mesenteroides SM을 이용하여 천년초 열매 추출액의 젖산 발효를 수행하였다. 천년초 열매의 수분함량은 71.7%, 가식부 65%로 나타났으며, 점질물 함량은 1.8%로 측정되었다. 천년초 열매 추출액은 열처리 시간이 증가함에 따라 점조도와 점탄성이 감소하였고, 퍼짐성은 증가하는 경향을 나타내었다. 또한 잔존하는 미생물은 $80^{\circ}C$에서 30분 열처리에 의해 사멸 되었으며, 천년초 열매색소는 비교적 안정한 값을 유지하였다. 고분자 dextran 생성을 위해 sucrose 함량을 20%까지 첨가하여 발효한 결과, sucrose 첨가량과 발효 시간이 증가할수록 점질물 함량 및 점조도는 증가하였으며 퍼짐성은 감소하였다. Sucrose을 20% 첨가하여 3일 동안 발효한 발효물의 점질물 함량이 4.8%로 가장 높게 나타났으며, sucrose 전환율은 90%를 보였다. 특히 sodium citrate 3% 첨가 시 발효 3일째 발효물의 점질물은 7%까지 증가하였고, 점조도 역시 가장 높은 값을 나타내었다. 발효된 천년초 열매 추출액은 생성된 고분자 dextran에 의한 높은 점성 성질을 나타내면서, 가공적성 증진과 probiotics가 강화된 기능성 발효물로 전환될 수 있었다. 또한 기능성이 강화된 천년초 발효 소재는 기능성식품 및 가공식품의 소재로서의 활용이 기대된다.

Keywords

References

  1. Kim TJ. Korean Resources Plants. Seoul National University Press, Seoul, Korea. pp. 140-141 (1996)
  2. Cha MN, Jun HI, Lee WJ, Kim MJ, Kim MK, Kim YS. Chemical composition and antioxidant activity of Korean cactus (Opuntia humifusa) fruit. Food Sci. Biotechnol. 22(2): 523-529 (2013) https://doi.org/10.1007/s10068-013-0110-0
  3. Askar A, El-Smamhy SK. Chemical composition of prickly pear fruit. Dtsch. Lebensm-Rundsch. 77: 279-281 (1981)
  4. Lee YC, Pyo YH, Ahn CK, Kim SH. Food functionality of Opunria ficus-indica var. cultivated in Jeju island. J. Food Sci. Nutr. 10: 103-110 (2005) https://doi.org/10.3746/jfn.2005.10.1.103
  5. Lee KS, Oh CS, Lee KY. Antioxidative effect of the fractions extracted from a cactus Chonnyuncho (Opuntia humifusa). Korean J. Food Sci. Technol. 37: 474-478 (2005)
  6. Kim MJ. Physicochemical characteristics of Jeungpyun by different addition ratios of prickly pear powder during storage. MS thesis, Sungshin Women's University, Seoul, Korea (2008)
  7. Park MK, Lee YJ, Kang ES. Hepatoprotective effect of Cheonnyuncho (Opuntia humifusa) extract in rats treated carbon tetrachloride. Korean J. Food Sci. Technol. 37: 822-826 (2005)
  8. Lee KS, Kim MG, Lee KY. Antimicrobial effect of the extracts of cactus Chounnyuncho (Opuntia humifusa) against food borne pathogens. J. Korean Soc. Food Sci. Nutr. 33: 1268-1272 (2004) https://doi.org/10.3746/jkfn.2004.33.8.1268
  9. Kwon DK, Song YJ. Effect of Opuntia humifusa supplementation on endurance exercise performance in rats fed a high-fat diet. Korean J. Exer. Nutr. 9: 183-188 (2005)
  10. Kim SY. Studies on the separation of antioxidative and antimicrobial compounds of Korean perennial cactus Opuntia ficus-indica var. (Cheonnyuncho). MS thesis, Hoseo University, Asan, Korea (2003)
  11. Kim KT, Choi AR, Lee KS, Joung YM, Lee KY. Quality characteristics of bread made from domestic Korean wheat flour containing cactus Cheonnyuncho (Opuntia humifusa) powder. Korean J. Food Cookery Sci. 23: 461-468 (2007)
  12. Jang SY. A study of the development of traditional rice cake with Cheonnyuncho fruit powder. MS thesis, Kyunggi University, Suwon, Korea (2010)
  13. Park SM, Ahn IS, Hong SM, Kim DS, Kwon DY, Yang HJ. The effects of the supplementation of Opuntia humifusa water extracts and methyl sulfonyl methane on the laying productivity, egg quality and sensory characteristics. J. Korean Soc. Food Sci. Nutr. 39: 294-300 (2010) https://doi.org/10.3746/jkfn.2010.39.2.294
  14. Lee SP, Whang K, Ha YD. Functional properties of mucilage and pigment extracted from Opuntia ficus-indica. J. Korean Soc. Food Sci. Nutr. 27: 821-826 (1998)
  15. Hasler CM. Functional Foods: the western perspective. Nutr. Rev. 54: S6-10 (1996)
  16. Kim JW. Quality improvement of traditional doenjang prepared from meju fermented with pure microbes. MS thesis, Chonbuk National University, Jeonju, Korea (2007)
  17. Lee WK. Functional soy yogurt production by Kimchi lactic acid bacteria. MS thesis, Mokpo National University, Mokpo, Korea (2009)
  18. Dziezak JD. Phosphates improve many food. J. Food Tech. 44: 80-86 (1990)
  19. Jeanes A. Dextran in encyclopedia of polymer science and technology. Wiley press, New York, NY, USA. pp. 805-824 (1966)
  20. Debelder AN. Dextran. pp. 513-542. In: Industrial Gums, Polysaccharides and Their Derivatives. Whistler RL, Bemiller JN (eds). Academic Press, Inc., New York, NY, USA (1973)
  21. Kwon KY. Studies on the mixed fermentation of Leuconostoc mesenteroides and Lactobacillus bulgaricus. MS thesis, Dankook University, Yongin, Korea (1993)
  22. Hepner G, Fried R, St. Jeor S, Fusetti L, Morin R. Hypocholesterolemic effect of yogurt and milk. Am. J. Clin. Nutr. 32: 19-24 (1979)
  23. KFDA. Korea Food & Drug Administration. Available from: http://fse.foodnara.go.kr/origin. Accessed May 21, 2009.
  24. Park SJ, Lee JH, Rhim JH, Kwon KS, Jang HG, Yu MY. The change of anthocyanin and spreadmeter value of strawberry jam by heating and preservation. Korean J. Food Sci. Technol. 26: 365-369 (1994)
  25. Sawaya WN. Khatchadourian HA, Safi WM, Al-Muhammad HM. Chemical characterization of prickly pear pulp, Opuntia ficusindica and the manufacturing of prickly pear jam. Int. J. Food Sci. Tech. 18: 183-193 (1983)
  26. Son MJ. Optimization of lactic acid fermentation of prickly pear (Opuntia ficus-indica var. saboten) extract and its application for jelly. MS thesis, Keimyung University, Deagu, Korea (2004)
  27. Shamala TR, Prasad MS. Preliminary studies on the production of high and low viscosity dextran by Leuconostoc spp. Process Biochem. 30: 237-241 (1995) https://doi.org/10.1016/0032-9592(95)85004-X
  28. Santos M, Teixeira J, Rodrigues A. Production of dextransucrase, dextran and fructose from sucrose using Leuconostoc mesenteroides NRRL B412(f). Biochem. Eng. J. 4: 177-188 (2000) https://doi.org/10.1016/S1369-703X(99)00047-9
  29. Kim DS, Thomas S, Fogler HS. Effects of pH and trace minerals on long-term starvation of Leuconostoc mesenteroides. Appl. Environ. Microb. 66: 976-981 (2000) https://doi.org/10.1128/AEM.66.3.976-981.2000
  30. Sarwat F, Qader SAU, Aman A, Ahmed N. Production & Characterization of a unique dextran from an indigenous Leuconostoc mesenteroides CMG713. Int. J. Biol. Sci. 4: 379-386 (2008)
  31. Jang KH, Choi JH, Lee JM, Lee JH, Jang SY, Jeong YJ. Fermentation characteristic of kefir beverage added fruit juice. Food Sci. Nutr. 7(3): 35-38 (2002)
  32. Lee YC, Hwang KH, Han DH, Kim SD. Compositions of Opuntia ficus-indica. Korean J. Food Sci. Technol. 29: 847-853 (1997)
  33. Hwang SG. Isolation of bacteria producing dextran from fermented kimchi and optimization of dextran production. MS thesis, Joongbu University, Geumsan, Korea (2007)
  34. Gilliland SE, Anna ED, Speck ML. Concentrated cultures of Leuconostoc citrovorum. Appl. Microbiol. 19: 890-893 (1970)
  35. Kim WJ, Kang KO, Kyung KH, Shin JI. Addition of salts and their mixtures for improvement of storage stability of kimchi. Korean J. Food Sci. Technol. 23: 188-191 (1991)
  36. Li L, Wei XY, Zhang YB, Du XH, Ren JP, Bilige M. Effect of medium composition on dextran produced by Leuconostoc mesenteroides subsp mesenteroides. China Dairy Industry 37(6): 16-19 (2009)

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